@misc{eprints55978,
           title = {IDENTIFIKASI SINGLE NUCLEOTIDE POLYMORPHISM (SNP) GEN
THROMBOSPONDIN-RELATED ANONYMOUS PROTEIN (TRAP)
PADA PENDERITA MALARIA falciparum DI WILAYAH KERJA
PUSKESMAS HANURA, KABUPATEN PESAWARAN,

PROVINSI LAMPUNG},
          author = {1518011004 MUHAMMAD IRFAN ADI SHULHAN},
         address = {UNIVERSITAS LAMPUNG},
       publisher = {FAKULTAS ILMU KEDOKTERAN},
            year = {2019},
             url = {http://digilib.unila.ac.id/55978/},
        abstract = {Background: Malaria is a disease caused by the Plasmodium parasite in
erythrocytes as evidenced by positive microscopic examination, there are antigens,
and the discovery of Deoxyribo Nucleid Acid / Ribonucleid Acid (DNA / RNA)
Plasmodium parasite on Polymerase Chain Reaction (PCR). Plasmodium
falciparum is one of the most deadly species of malaria, and there are many genes
that support this parasite to infect, so there are so many genetic variations produced
by one gene.
Method: This type of research uses a survey research design with descriptive
method. The study sample was obtained from 18 Stored Archived Biological
Materials (ABM). This research was conducted using the PCR method which was
analyzed by gel electrophoresis and continued to the sequencing method to detect
genetic variations of the Plasmodium falciparum Thrombospondin-related
anonymous protein (PfTRAP) gene.
Result: There were 18 samples that had been carried out nested PCR, then obtained
variations in the length of the band in each sample
Conclusion: There are genetic variation in the Thrombospondin-related
anonymous protein (TRAP) gene.
Keyword: Malaria, Plasmodium Falciparum Thrombospondin-related anonymous
protein (PfTRAP), Polymerase Chain Reaction (PCR), Sequencing.
Latar Belakang: Malaria merupakan penyakit yang disebabkan oleh parasit
Plasmodium di dalam eritrosit dibuktikan dengan pemeriksaan mikroskopis yang
positif, terdapat antigen, serta ditemukannya Deoxyribo Nucleid Acid/ Ribonucleid
Acid (DNA/RNA) parasit Plasmodium pada pemeriksaan Polymerase Chain
Reaction (PCR). Plasmodium falciparum adalah salah satu spesies yang paling
banyak menyebabkan kasus malaria dan paling mematikan, serta terdapat banyak gen
yang mendukung parasit ini untuk menginfeksi, sehingga banyak sekali variasi
genetik yang dihasilkan oleh satu gen.
Metode: Jenis penelitian ini menggunakan rancangan penelitian survey dan
bersifat deskriptif. Sampel penelitian diperoleh dari Bahan Biologi Tersimpan
(BBT) sebanyak 18 sampel. Penelitian ini dilakukan dengan menggunakan metode
PCR yang dianalisis dengan gel elektroforesis serta dilanjutkan ke metode
sekuensing untuk mendeteksi variasi genetik gen Plasmodium falciparum
Thrombospondin-related anonymous protein (PfTRAP).
Hasil: Terdapat 18 sampel yang telah dilakukan nested PCR, kemudian diperoleh
variasi panjang pita pada setiap sampel.
Kesimpulan: Terdapat variasi genetik gen Thrombospondin-related anonymous
protein (TRAP).
Kata Kunci: Malaria, Plasmodium Falciparum Thrombospondin-related
anonymous protein (PfTRAP), Polymerase Chain Reaction (PCR), Sekuensing.}
}