?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=IDENTIFIKASI+SINGLE+NUCLEOTIDE+POLYMORPHISM+(SNP)+GEN%0D%0ATHROMBOSPONDIN-RELATED+ANONYMOUS+PROTEIN+(TRAP)%0D%0APADA+PENDERITA+MALARIA+falciparum+DI+WILAYAH+KERJA%0D%0APUSKESMAS+HANURA%2C+KABUPATEN+PESAWARAN%2C%0D%0A%0D%0APROVINSI+LAMPUNG&rft.creator=MUHAMMAD+IRFAN+ADI+SHULHAN%2C+1518011004&rft.subject=+&rft.description=Background%3A+Malaria+is+a+disease+caused+by+the+Plasmodium+parasite+in%0D%0Aerythrocytes+as+evidenced+by+positive+microscopic+examination%2C+there+are+antigens%2C%0D%0Aand+the+discovery+of+Deoxyribo+Nucleid+Acid+%2F+Ribonucleid+Acid+(DNA+%2F+RNA)%0D%0APlasmodium+parasite+on+Polymerase+Chain+Reaction+(PCR).+Plasmodium%0D%0Afalciparum+is+one+of+the+most+deadly+species+of+malaria%2C+and+there+are+many+genes%0D%0Athat+support+this+parasite+to+infect%2C+so+there+are+so+many+genetic+variations+produced%0D%0Aby+one+gene.%0D%0AMethod%3A+This+type+of+research+uses+a+survey+research+design+with+descriptive%0D%0Amethod.+The+study+sample+was+obtained+from+18+Stored+Archived+Biological%0D%0AMaterials+(ABM).+This+research+was+conducted+using+the+PCR+method+which+was%0D%0Aanalyzed+by+gel+electrophoresis+and+continued+to+the+sequencing+method+to+detect%0D%0Agenetic+variations+of+the+Plasmodium+falciparum+Thrombospondin-related%0D%0Aanonymous+protein+(PfTRAP)+gene.%0D%0AResult%3A+There+were+18+samples+that+had+been+carried+out+nested+PCR%2C+then+obtained%0D%0Avariations+in+the+length+of+the+band+in+each+sample%0D%0AConclusion%3A+There+are+genetic+variation+in+the+Thrombospondin-related%0D%0Aanonymous+protein+(TRAP)+gene.%0D%0AKeyword%3A+Malaria%2C+Plasmodium+Falciparum+Thrombospondin-related+anonymous%0D%0Aprotein+(PfTRAP)%2C+Polymerase+Chain+Reaction+(PCR)%2C+Sequencing.%0D%0ALatar+Belakang%3A+Malaria+merupakan+penyakit+yang+disebabkan+oleh+parasit%0D%0APlasmodium+di+dalam+eritrosit+dibuktikan+dengan+pemeriksaan+mikroskopis+yang%0D%0Apositif%2C+terdapat+antigen%2C+serta+ditemukannya+Deoxyribo+Nucleid+Acid%2F+Ribonucleid%0D%0AAcid+(DNA%2FRNA)+parasit+Plasmodium+pada+pemeriksaan+Polymerase+Chain%0D%0AReaction+(PCR).+Plasmodium+falciparum+adalah+salah+satu+spesies+yang+paling%0D%0Abanyak+menyebabkan+kasus+malaria+dan+paling+mematikan%2C+serta+terdapat+banyak+gen%0D%0Ayang+mendukung+parasit+ini+untuk+menginfeksi%2C+sehingga+banyak+sekali+variasi%0D%0Agenetik+yang+dihasilkan+oleh+satu+gen.%0D%0AMetode%3A+Jenis+penelitian+ini+menggunakan+rancangan+penelitian+survey+dan%0D%0Abersifat+deskriptif.+Sampel+penelitian+diperoleh+dari+Bahan+Biologi+Tersimpan%0D%0A(BBT)+sebanyak+18+sampel.+Penelitian+ini+dilakukan+dengan+menggunakan+metode%0D%0APCR+yang+dianalisis+dengan+gel+elektroforesis+serta+dilanjutkan+ke+metode%0D%0Asekuensing+untuk+mendeteksi+variasi+genetik+gen+Plasmodium+falciparum%0D%0AThrombospondin-related+anonymous+protein+(PfTRAP).%0D%0AHasil%3A+Terdapat+18+sampel+yang+telah+dilakukan+nested+PCR%2C+kemudian+diperoleh%0D%0Avariasi+panjang+pita+pada+setiap+sampel.%0D%0AKesimpulan%3A+Terdapat+variasi+genetik+gen+Thrombospondin-related+anonymous%0D%0Aprotein+(TRAP).%0D%0AKata+Kunci%3A+Malaria%2C+Plasmodium+Falciparum+Thrombospondin-related%0D%0Aanonymous+protein+(PfTRAP)%2C+Polymerase+Chain+Reaction+(PCR)%2C+Sekuensing.&rft.publisher=FAKULTAS+ILMU+KEDOKTERAN&rft.date=2019&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F55978%2F1%2F1.%2520ABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F55978%2F2%2F2.%2520SKRIPSI%2520FULL%2520TEKS.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F55978%2F3%2F3.%2520SKRIPSI%2520FULL%2520TEKS%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++MUHAMMAD+IRFAN+ADI+SHULHAN%2C+1518011004++(2019)+IDENTIFIKASI+SINGLE+NUCLEOTIDE+POLYMORPHISM+(SNP)+GEN+THROMBOSPONDIN-RELATED+ANONYMOUS+PROTEIN+(TRAP)+PADA+PENDERITA+MALARIA+falciparum+DI+WILAYAH+KERJA+PUSKESMAS+HANURA%2C+KABUPATEN+PESAWARAN%2C+PROVINSI+LAMPUNG.++FAKULTAS+ILMU+KEDOKTERAN%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F55978%2F