%0 Generic
%A MUHAMMAD IRFAN ADI SHULHAN, 1518011004
%C UNIVERSITAS LAMPUNG
%D 2019
%F eprints:55978
%I FAKULTAS ILMU KEDOKTERAN
%T IDENTIFIKASI SINGLE NUCLEOTIDE POLYMORPHISM (SNP) GEN  THROMBOSPONDIN-RELATED ANONYMOUS PROTEIN (TRAP)  PADA PENDERITA MALARIA falciparum DI WILAYAH KERJA  PUSKESMAS HANURA, KABUPATEN PESAWARAN,    PROVINSI LAMPUNG
%U http://digilib.unila.ac.id/55978/
%X Background: Malaria is a disease caused by the Plasmodium parasite in  erythrocytes as evidenced by positive microscopic examination, there are antigens,  and the discovery of Deoxyribo Nucleid Acid / Ribonucleid Acid (DNA / RNA)  Plasmodium parasite on Polymerase Chain Reaction (PCR). Plasmodium  falciparum is one of the most deadly species of malaria, and there are many genes  that support this parasite to infect, so there are so many genetic variations produced  by one gene.  Method: This type of research uses a survey research design with descriptive  method. The study sample was obtained from 18 Stored Archived Biological  Materials (ABM). This research was conducted using the PCR method which was  analyzed by gel electrophoresis and continued to the sequencing method to detect  genetic variations of the Plasmodium falciparum Thrombospondin-related  anonymous protein (PfTRAP) gene.  Result: There were 18 samples that had been carried out nested PCR, then obtained  variations in the length of the band in each sample  Conclusion: There are genetic variation in the Thrombospondin-related  anonymous protein (TRAP) gene.  Keyword: Malaria, Plasmodium Falciparum Thrombospondin-related anonymous  protein (PfTRAP), Polymerase Chain Reaction (PCR), Sequencing.  Latar Belakang: Malaria merupakan penyakit yang disebabkan oleh parasit  Plasmodium di dalam eritrosit dibuktikan dengan pemeriksaan mikroskopis yang  positif, terdapat antigen, serta ditemukannya Deoxyribo Nucleid Acid/ Ribonucleid  Acid (DNA/RNA) parasit Plasmodium pada pemeriksaan Polymerase Chain  Reaction (PCR). Plasmodium falciparum adalah salah satu spesies yang paling  banyak menyebabkan kasus malaria dan paling mematikan, serta terdapat banyak gen  yang mendukung parasit ini untuk menginfeksi, sehingga banyak sekali variasi  genetik yang dihasilkan oleh satu gen.  Metode: Jenis penelitian ini menggunakan rancangan penelitian survey dan  bersifat deskriptif. Sampel penelitian diperoleh dari Bahan Biologi Tersimpan  (BBT) sebanyak 18 sampel. Penelitian ini dilakukan dengan menggunakan metode  PCR yang dianalisis dengan gel elektroforesis serta dilanjutkan ke metode  sekuensing untuk mendeteksi variasi genetik gen Plasmodium falciparum  Thrombospondin-related anonymous protein (PfTRAP).  Hasil: Terdapat 18 sampel yang telah dilakukan nested PCR, kemudian diperoleh  variasi panjang pita pada setiap sampel.  Kesimpulan: Terdapat variasi genetik gen Thrombospondin-related anonymous  protein (TRAP).  Kata Kunci: Malaria, Plasmodium Falciparum Thrombospondin-related  anonymous protein (PfTRAP), Polymerase Chain Reaction (PCR), Sekuensing.