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<http://digilib.unila.ac.id/id/eprint/56151> <http://purl.org/dc/terms/title> "IDENTIFIKASI SINGLE NUCLEOTIDE POLYMORPHISM (SNP) PADA\r\nGEN CIRCUMSPOROZOITE PROTEIN Plasmodium falciparum DARI\r\nPENDERITA MALARIA DI WILAYAH KERJA PUSKESMAS HANURA,\r\n\r\nPESAWARAN, LAMPUNG"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/eprint/56151> <http://purl.org/ontology/bibo/abstract> "Background: Malaria is an infectius disease caused by Plasmodium sp.\r\nPlasmodium falciparum is the most deadly cause. The treatment for malaria with\r\nArtemisinin-based Combination Therapy (ACT) can cause resistance, so a new\r\neffort is needed to reduce the morbidity of malaria. A better prevention method\r\nfor preventing malaria is to use vaccine. The RTS,S/AS01 vaccine is the first\r\ngeneration of malaria vaccine that has been applied in Ghana, Kenya and Malawi\r\nsince early 2018. The vaccine consists of circumsporozoite protein antigen and\r\nhepatitis B antigen. This vaccine has been shown to provide partial protection to\r\nchildren and has reach efficacy by 80%.\r\nMethod: This type of research is a descriptive research with survey design. The\r\nsample of research was obtained from Biological Saved Materials (BSM) as many\r\nas 53 samples. The examination was performed using by PCR method and\r\nfollowed by a sequencing process to detect SNP.\r\nResult: The results showed that only 16 samples had high DNA concentration\r\nand were successfully performed by PCR with the results of electrophoresis\r\nshowing that the samples had the same base pair length according to the target\r\nlength of the circumsporozoite protein gene and the sequencing results showed\r\nthere is no difference in nucleotide base sequences compared to references on\r\nPf3D7 isolates from Gene Bank.\r\nConclusion: There is no SNP on the circumsporozite protein gene in Plasmodium\r\nfalciparum from malaria patients in the working area of Primary Health Care\r\nHanura, Pesawaran, Lampung.\r\nKeyword: Circumsporozoite protein, Plasmodium falciparum.\r\nLatar Belakang: Malaria merupakan penyakit infeksi yang disebabkan oleh\r\nparasit Plasmodium sp. Plasmodium falciparum adalah penyebab yang paling\r\nmematikan. Pengobatan penyakit malaria menggunakan Artemisinin-based\r\nCombination Therapy (ACT) dapat menyebabkan resistensi sehingga diperlukan\r\nupaya baru untuk menurunkan angka kesakitan malaria. Sebuah metode\r\npencegahan yang lebih baik untuk mencegah kejadian malaria adalah\r\nmenggunakan vaksin. Vaksin RTS,S/AS01 merupakan vaksin malaria generasi\r\npertama yang telah diterapkan di Ghana, Kenya dan Malawi sejak awal tahun\r\n2018. Vaksin tersebut terdiri dari komponen antigen circumsporozoite protein dan\r\nhepatitis B. Vaksin ini telah terbukti memberikan perlindungan parsial kepada\r\nanak-anak dan memiliki efikasi sebesar 80%.\r\nMetode: Jenis penelitian ini menggunakan rancangan penelitian survey dan\r\nbersifat deskriptif. Sampel penelitian diperoleh dari Bahan Biologi Tersimpan\r\n(BBT) sebanyak 53 sampel. Pemeriksaan dilakukan dengan menggunakan metode\r\nPCR yang dilanjutkan dengan proses sekuensing untuk mendeteksi adanya SNP.\r\nHasil: Hasil penelitian menunjukkan bahwa hanya 16 sampel yang memiliki\r\nkonsentrasi DNA yang tinggi, selanjutnya sampel tersebut dilakukan PCR dengan\r\nhasil elektroforesis menunjukkan bahwa seluruh sampel memiliki panjang basa\r\nyang sama sesuai target panjang gen circumsporozoite protein dan hasil\r\nsekuensing tidak menunjukkan adanya perbedaan urutan basa nukleotida\r\ndibandingkan dengan referensi pada isolat Pf3D7 dari Gene Bank.\r\nKesimpulan: Tidak terdapat SNP pada gen circumsporozoite protein Plasmodium\r\nfalciparum dari penderita malaria di wilayah kerja Puskesmas Hanura, Pesawaran,\r\nLampung.\r\nKata Kunci: Circumsporozoite protein, Plasmodium falciparum."^^<http://www.w3.org/2001/XMLSchema#string> .
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