?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=KARAKTERISASI+MOLEKULER+BAKTERI+ISOLAT+LOKAL+%0D%0ATERPILIH+ASAL+LIMBAH+SINGKONG+DENGAN+METODE+16S+rRNA&rft.creator=WIDYA+KUSUMA%2C+1517011119&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.description=Lampung+province+has+a+number+of+cassava+production+at+7%2C384%2C084+tons.+The+%0D%0Aincreasing+of+cassava+production+related+to+the+increase+in+cassava+waste%0D%0Aautomatically.+Indirectly+one+of+the+main+waste+of+cassava+industry+is+cassava+peel.%0D%0ACassava+peel+consists+of+components+such+as+cellulose+and+amylum%2C+so+that%2C+this+%0D%0Amaterial+could+be+as+a+medium+for+bacterial+growth+such+as+cellulolytic+and+%0D%0Aamylolytic+bacteria.+This+research+aims+to+characterize+the+molecular+of+local+%0D%0Aisolate+bacteria+that+selected+in+cassava+waste+indigenously%2C+conducted+by+16S%0D%0ArRNA+method+and+identify+the+type+or+species+through+phylogenetic+analysis.+The+%0D%0Aresults+of+DNA+isolation+obtained+DNA+from+cell+culture+of+SCWB1+and+SCWB17+%0D%0Awere+33.9+ng%2F%C2%B5l+and+218+ng%2F%C2%B5l+respectively.+The+purity+of+isolated+DNA+was+%0D%0Aexamined+by+comparing+absorbance+of+%CE%BB260%2F280+and+%CE%BB260%2F230.+Our+result+show+%0D%0Athat+the+value+of+comparison+for+SCWB1+were+2%2C061+and+2%2C519%2C+since+for+SCWB17%0D%0Awere+1%2C904+and+1%2C585+respectively.+Amplicons+appear+for+both+of+isolates+had+DNA+%0D%0Afragment+at+%C2%B1+1500+bp.+Based+on+BLAST+analysis+on+NCBI+and+phylogenetic+tree+%0D%0Aconstruction+it+was+noted+that+SCWB1+isolates+have+a+percent+identity+of+97.73%25+%0D%0Aconnected+to+Bacillus+cereus+strains+IAM+12605+and+SCWB17+isolates+has+a+%0D%0Apercent+identity+of+97.90%25+connected+to+Klebsiella+variicola+strains+F2R9.%0D%0AKeywords%3A+Cassava+waste%2C+16S+rRNA%2C+Bacillus+cereus%2C+Klebsiella+variicola%0D%0A%0D%0AProvinsi+Lampung+memiliki+angka+produksi+singkong+sebesar+7.384.084+ton.+%0D%0AMeningkatnya+produksi+singkong+menyebabkan+peningkatan+pada+jumlah+limbah+%0D%0Asingkong.+Limbah+utama+dari+industri+singkong+adalah+kulit+singkong.+Kulit+%0D%0Asingkong+mengandung+komponen+seperti+selulose+dan+amilum%2C+sehingga+dapat+%0D%0Adijadikan+suatu+media+bagi+pertumbuhan+bakteri+seperti+bakteri+selulolitik+dan+%0D%0Aamilolitik.+Penelitian+ini+bertujuan+untuk+mengkarakterisasi+molekuler+isolat+lokal+%0D%0Aterpilih+asal+limbah+singkong+indigenous+yang+dilakukan+dengan+metode+16S+%0D%0ArRNA+dan+ditentukan+jenis+atau+spesiesnya+melalui+analisis+filogenetik.+Hasil+%0D%0Aisolasi+DNA+menunjukkan+bahwa+isolat+SCWB1+memiliki+konsentrasi+sebesar+%0D%0A33%2C9+ng%2F%C2%B5l+dan+isolat+SCWB17+memiliki+konsentrasi+sebesar+218+ng%2F%C2%B5l.+Hasil+%0D%0Akemurnian+DNA+dari+isolat+SCWB1+adalah+2%2C061+pada+%CE%BB260%2F280+dan+2%2C519+pada+%0D%0A%CE%BB260%2F230.+Sementara+pada+isolat+SCWB17+diperoleh+hasil+yaitu+1%2C904+pada+%0D%0A%CE%BB260%2F280+dan+1%2C585+pada+%CE%BB260%2F230.+Amplikon+menunjukkan+bahwa+kedua+isolat+%0D%0Amemiliki+fragmen+DNA+yang+berukuran+%C2%B1+1500+bp.+Berdasarkan+analisis+BLAST%0D%0Apada+situs+NCBI+dan+konstruksi+pohon+filogenetik+dapat+disimpulkan+bahwa+isolat+%0D%0ASCWB1+memiliki+nilai+percent+identity+97%2C73%25+terhadap+Bacillus+cereus+strain+%0D%0AIAM+12605+dan+isolat+SCWB17+memiliki+nilai+percent+identity+97%2C90%25+terhadap+%0D%0AKlebsiella+variicola+strain+F2R9.%0D%0AKata+kunci+%3A+Limbah+singkong%2C+16S+rRNA%2C+Bacillus+cereus%2C+Klebsiella+variicola%0D%0A&rft.publisher=Fakultas+Matematika+dan+Ilmu+Pengetahuan+Alam&rft.date=2019&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F56565%2F1%2F1.%2520ABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F56565%2F3%2F2.%2520SKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F56565%2F2%2F3.%2520SKRIPSI%2520FULL%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++WIDYA+KUSUMA%2C+1517011119++(2019)+KARAKTERISASI+MOLEKULER+BAKTERI+ISOLAT+LOKAL+TERPILIH+ASAL+LIMBAH+SINGKONG+DENGAN+METODE+16S+rRNA.++Fakultas+Matematika+dan+Ilmu+Pengetahuan+Alam%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F56565%2F