?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=PENINGKATAN+STABILITAS+ENZIM+SELULASE+DARI+Aspergillus+%0D%0Afumigatus+DENGAN+MODIFIKASI+KIMIA+MENGGUNAKAN+%0D%0ASITRAKONAT+ANHIDRIDA&rft.creator=ANNISA+MAWADDAH%2C+1517011132&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.description=Cellulase+is+a+complex+enzyme+that+gradually+hydrolyze+the+cellulose+chain+into+%0D%0Aglucose+and+widely+used+in+industrial+processes.+The+use+of+the+enzyme+in+industry+%0D%0Arequires+enzymes+that+are+stabile+to+extreme+temperatures+and+pH.+The+objective+%0D%0Aof+this+research+is+to+increase+the+stability+of+the+cellulase+produced+by+Aspergillus+%0D%0Afumigatus+using+chemical+modification+with+citraconic+anhydride.+The+production%2C+%0D%0Aisolation%2C+purification%2C+modification+and+characterization+have+been+conducted+to+%0D%0Aachieve+that+objectives.+Characterization+of+purified+and+modified+cellulase+as+a+%0D%0Aresult+consist+of+determination+of+optimum+temperature+and+pH%2C+KM+and+Vmax%0D%0Avalues+and+enzymatic+thermal+stability.+The+results+showed+that+the+specific+%0D%0Aactivity+of+cellulase+was+354.6+U%2Fmg%2C+increase+5.1+times+compared+to+crude+%0D%0Aextracts+of+enzymes+which+had+specific+activities+of+68.9+U%2Fmg.+Purified+cellulase+%0D%0Ahas+an+optimum+pH+5.5%3B+optimum+temperature+at+55%C2%BAC%3B+KM+%3D+19.75+mg%2FmL+%0D%0Asubstrate%3B+Vmax+%3D+2.223+%C2%B5mol%2FmL.minutes.+The+thermal+stability+test+of+purified+%0D%0Acellulase+at+temperature+55%C2%BAC+for+100+minute+has+ki+value+%3D+0.0148+minutes-1%0D%0A%3B+half%EF%BF%BDlife+(t1%2F2)+%3D+46.82+minutes+and+%CE%94Gi+%3D+103.3+kJ%2Fmol.+Modified+enzymes+with+%0D%0Acitraconic+anhydride+30%3B+40+and+50+%C2%B5L+have+an+optimum+pH+of+5.5%3B+optimum+%0D%0Atemperature+of+55%C2%BAC%3B+respectively+had+KM+in+a+row+of+7.089%3B+11.32%3B+and+11.14+%0D%0Amg%2FmL+substrate%3B+Vmax%3A+1.272%3B+1.965%3B+2.136+%C2%B5mol%2FmL.minutes.+The+thermal+%0D%0Astability+test+of+modified+cellulase+at+temperature+55%C2%BAC+for+100+minute+has+ki%0D%0Avalue%3A+0.0069%3B+0.0061%3B+and+0.0028+minutes-1%0D%0Aand+half-life+(t1%2F2)%3A+100.4%3B+115.5%3B+and+%0D%0A247.5+minutes+respectively.+Since+the+stabilized+enzymes+have+%CE%94Gi%3A+105.3%3B+105.7%3B+%0D%0Aand+107.8+kJ%2Fmol+respectively.+Based+on+the+decrease+the+value+of+ki%0D%0A%2C+an+increase+%0D%0Ain+half-life+(t1%2F2)+and+%CE%94Gi+value%2C+it+is+known+that+modification+using+citraconic+%0D%0Aanhydride+can+increase+the+stability+of+the+cellulase+from+Aspergillus+fumigatus.%0D%0AKeyword+%3A+Cellulase%2C+Aspergillus+fumigatus%2C+Chemical+Modification%2C+Citraconic+%0D%0AAnhydride%0D%0A%0D%0ASelulase+adalah+enzim+kompleks+yang+memotong+secara+bertahap+rantai+selulosa+%0D%0Amenjadi+glukosa+dan+banyak+digunakan+pada+proses+industri.+Penggunaan+enzim+%0D%0Adalam+industri+memerlukan+enzim+yang+tahan+pada+suhu+dan+pH+yang+ekstrim.+%0D%0AOleh+karena+itu%2C+perlu+dilakukan+peningkatan+kestabilan+enzim+selulase.+Penelitian+%0D%0Aini+bertujuan+untuk+meningkatkan+stabilitas+enzim+selulase+dari+Aspergillus+%0D%0Afumigatus+dengan+modifikasi+kimia+menggunakan+sitrakonat+anhidrida.+Produksi%2C+%0D%0Aisolasi%2C+pemurnian%2C+modifikasi+dan+karakterisasi+dilakukan+untuk+mencapai+tujuan+%0D%0Atersebut.+Karakterisasi+enzim+selulase+hasil+pemurnian+dan+hasil+modifikasi+%0D%0Ameliputi+penentuan+suhu+dan+pH+optimum%2C+nilai+KM+dan+Vmaks+serta+uji+stabilitas+%0D%0Atermal+enzim.+Hasil+penelitian+menunjukkan+bahwa+aktivitas+spesifik+enzim+%0D%0Aselulase+354%2C6+U%2Fmg%2C+meningkat+5%2C1+kali+dibandingkan+dengan+ekstrak+kasar+%0D%0Aenzim+yang+mempunyai+aktivitas+spesifik+68%2C9+U%2Fmg.+Enzim+selulase+hasil+%0D%0Apemurnian+mempunyai+pH+optimum+5%2C5%3B+suhu+optimum+55%C2%BAC%3B+KM+%3D+19%2C75+mg%2FmL+%0D%0Asubstrat%3B+Vmaks+%3D+2%2C223+%C2%B5mol%2FmL.menit.+Uji+stabilitas+termal+enzim+selulase+hasil+%0D%0Apemurnian+pada+suhu+55%C2%BAC+selama+100+menit+memiliki+nilai+ki+%3D+0%2C0148+menit-1%0D%0A%3B+%0D%0Awaktu+paruh+(t1%2F2)+%3D+46%2C82+menit+dan+%CE%94Gi+%3D+103%2C3+kJ%2Fmol.+Enzim+hasil+modifikasi%0D%0Adengan+sitrakonat+anhidrida+30%3B+40+dan+50+%C2%B5L+mempunyai+pH+optimum+5%2C5%3B+suhu+%0D%0Aoptimum+55%C2%BAC%3B+KM+berturut-turut+7%2C089%3B+11%2C32%3B+dan+11%2C14+mg%2FmL+substrat%3B+Vmaks%0D%0Aberturut-turut+sebesar+1%2C272%3B+1%2C965%3B+2%2C136+%C2%B5mol%2FmL.menit.+Uji+stabilitas+termal+%0D%0Aenzim+selulase+hasil+modifikasi+pada+suhu+55%C2%BAC+selama+100+menit+memiliki+nilai%0D%0Aki+berturut-turut+sebagai+berikut%3A+0%2C0069%3B+0%2C0061%3B+dan+0%2C0028+menit-1%0D%0A%3B+waktu+paruh+%0D%0A(t1%2F2)+berturut-turut+sebagai+berikut%3A+100%2C4%3B+115%2C5%3B+dan+247%2C5+menit%3B+%CE%94Gi+berturut%EF%BF%BDturut+sebagai+berikut%3A+105%2C3%3B+105%2C7%3B+dan+107%2C8+kJ%2Fmol.+Berdasarkan+penurunan+%0D%0Anilai+ki%0D%0A%2C+peningkatan+waktu+paruh+(t1%2F2)+dan+nilai+%CE%94Gi%0D%0A%2C+diketahui+bahwa+modifikasi+%0D%0Amenggunakan+sitrakonat+anhidrida+dapat+meningkatkan+stabilitas+enzim+selulase+%0D%0Adari+Aspergillus+fumigatus.+%0D%0AKata+kunci+%3A+S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