?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=SOLASI+DAN+KARAKTERISASI+BAKTERI+ASAM+LAKTAT%0D%0APENGHASIL+EKSOPOLISAKARIDA+DARI+FERMENTASI+KEFIR%0D%0AKOLOSTRUM&rft.creator=Intan+Tsamrotul+Fu%E2%80%99adah%2C+1517011102&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.description=Eksopolisakarida+(EPS)+merupakan+polisakarida+yang+disekresikan+oleh+mikroba%0D%0Akeluar+dinding+sel%2C+salah+satunya+oleh+bakteri+asam+laktat+(BAL).+Kefir+kolostrum%0D%0Amerupakan+minuman+fermentasi+yang+berpotensi+sebagai+sumber+BAL.+Penelitian%0D%0Aini+bertujuan+untuk+mendapatkan+isolat+BAL+dari+kefir+kolostrum+dan%0D%0Amengkarakterisasi+BAL+penghasil+EPS.+Untuk+mencapai+tujuan+tersebut%2C+metode%0D%0Ayang+digunakan+yaitu+dengan+teknik+pour+plate+dan+streak+plate.+Isolat-isolat%0D%0Ayang+diperoleh+diuji+kemampuannya+dalam+menghasilkan+EPS+melalui%0D%0Apengamatan+morfologi+pada+media+MRSA.+Produksi+EPS+dilakukan+dengan%0D%0Amenumbuhkan+kultur+pada+media+MRSB+dan+dipisahkan+dengan+metode%0D%0Asentrifugasi.+Analisis+kandungan+kadar+total+gula+EPS+diuji+dengan+metode+fenol-+sulfat+dan+karakter+isolat+ditentukan+melalui+identifikasi+makroskopik+dan%0D%0Amikroskopik.+Hasil+penelitian+menunjukkan+ada+13+isolat+BAL+penghasil+EPS%0D%0Ayaitu+BEK+1-13+dengan+9+isolat+berbentuk+mucoid+dan+4+isolat+ropy.+Produksi%0D%0AEPS+tertinggi+diperoleh+dari+isolat+BEK+13+yaitu+4840+mg%2FL+dengan+kadar+gula%0D%0AEPS+tertinggi+diperoleh+dari+isolat+BEK+1+sebesar+579.56+mg%2FL.+Karakterisasi%0D%0Abiokimia+menunjukkan+13+isolat+berbeda+satu+sama+lain+yakni+12+isolat+gram%0D%0Anegatif+dan+1+isolat+gram+positif%2C+11+isolat+negatif+endospora+dan+2+isolat+positif%0D%0Aendospora+serta+semua+isolat+menunjukkan+uji+katalase+positif.+Kata+Kunci%3A+Bakteri+Asam+Laktat%2C+Eksopolisakarida%2C+Kefir+Kolostrum%0D%0AExopolysaccharide+(EPS)+is+a+polysaccharide+secreted+by+microbes+out+of+the+cell%0D%0Awall%2C+for+instance+is+lactic+acid+bacteria+(LAB).+Colostrum+kefir+is+a+fermented%0D%0Abeverage+that+has+the+potential+as+a+source+of+LAB.+This+research+aims+to+obtain%0D%0ALAB+isolates+from+colostrum+kefir+and+characterize+EPS-producing+LAB.+The%0D%0Amethod+used+are+the+pour+plate+and+streak+plate+techniques.+The+isolates+obtained%0D%0Awere+tested+for+their+ability+to+produce+EPS+through+morphological+observations%0D%0Aon+MRSA+media.+EPS+production+is+done+by+growing+cultures+on+MRSB+media%0D%0Aand+separated+by+centrifugation+methods.+Total+EPS+sugar+content+was+tested+by%0D%0Athe+phenol-sulfate+method+and+the+isolate+character+was+determined+through%0D%0Amacroscopic+and+microscopic+identification.+The+result+show+that+13+isolates+of%0D%0ALAB+that+produce+EPS%2C+namely+BEK+1-13+which+is+9+isolates+mucoid+and+4%0D%0Aisolates+ropy.+The+highest+EPS+produced+by+BEK+13+isolate+with+4840+mg%2FL+and%0D%0Athe+highest+sugar+total+of+EPS+produced+by+BEK+1+isolates+which+was+579.56%0D%0Amg%2FL.+Biochemical+characteristic+showed+that+13+isolates+differed+from+each%0D%0Aother%2C+namely+12+gram+negative+isolates+and+1+gram+positive+isolate+where+as+11%0D%0Anegative+endospores+isolates+and+2+endospores+positive+isolates+and+also+all%0D%0Aisolates+showed+positive+catalase+tests.%0D%0AKeywords%3A+Lactic+Acid+Bacteria%2C+Exopolysaccharides%2C+Colostrum+Kefir%0D%0A%0D%0A%0D%0A&rft.publisher=Fakultas+Matematika+dan+Ilmu+Pengetahuan+Alam&rft.date=2019&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F56746%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F56746%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F56746%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++Intan+Tsamrotul+Fu%E2%80%99adah%2C+1517011102++(2019)+SOLASI+DAN+KARAKTERISASI+BAKTERI+ASAM+LAKTAT+PENGHASIL+EKSOPOLISAKARIDA+DARI+FERMENTASI+KEFIR+KOLOSTRUM.++Fakultas+Matematika+dan+Ilmu+Pengetahuan+Alam%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F56746%2F