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primary topic: http://digilib.unila.ac.id/id/eprint/5678

http://digilib.unila.ac.id/id/eprint/5678
type: Article
title: REVALIDASI PENGARUH VARIASI KOMPOSISI MEDIUM KULTUR TERHADAP PRODUKSI ENZIM AMILASE DARI Bacillus sp. STRAIN LTE-6 REVALIDASI EFFECT OF MEDIUM COMPOSITION VARIATIONS OF CULTURE OF PRODUCTION enzyme amylase Bacillus sp. STRAIN LTE-6
abstract: ABSTRAK Penelitian ini bertujuan untuk uji ulang penelitian yang telah dilakukan pada penelitian sebelumnya pada pengaruh variasi komposisi medium kultur terhadap produksi enzim amilase. Penelitian ini menggunakan bakteri amilolitik isolat lokal LTE-6 yang telah diisolasi dari penelitian sebelumnya yang merupakan bakteri penghasil enzim amilase. Variasi komposisi yang digunakan antara lain : sumber C (glukosa, arabinosa, fruktosa dan gula), variasi sumber N (pepton, NaNO3, NH4Cl dan ekstrak ragi), ion logam (Fe, Mn, Mg, Zn) dan variasi pH (5, 6, 7 dan 8). Pada penelitian sebelumnya, dari perlakuan pada variasi sumber N, C, ion logam dan pH diperoleh bahwa kondisi yang optimum bagi pertumbuhan sel dan produksi enzim dari isolat LTE-6 sumber N yaitu pepton 0,5% (w/v), sumber C yaitu gula 0,5% (w/v), sumber ion logam yaitu Fe 0,5% (w/v) pada pH 6.0, dengan aktivitas unit secara berurut yaitu 9,8 U/ml, 10,83 U/ml, 9,5 U/ml, 8,7 U/ml pada usia kultur 24, 48, 24, dan 24 jam, dengan OD sel 1.91, 3.75, 3.32, 1.45. Pada penelitian uji ulang ini, kondisi optimum bagi pertumbuhan sel dan produksi enzim amilase dari isolat LTE-6 secara berurutan yakni: sumber C adalah gula 0,5% (w/v), sumber N adalah pepton 0,5% (w/v) ion logam adalah Mg 0,01% (w/v), dan pada pH 7.0. Pada masing-masing kondisi tersebut, diperoleh perubahan nilai aktivitas unit enzim amilase isolat ini secara berurut yaitu 3,7 U/ml; 9,59 U/ml; 9,88 U/ml; 9,9 U/ml, pada usia kultur masing-masing 48 jam, dengan OD sel 2,45; 4,08; 5,12; 3,58. Dengan kata lain, penelitian uji ulang ini lebih baik karena memperoleh nilai aktivitas unit lebih tinggi dari penelitian sebelumnya. ABSTRAK This study aims to retest studies conducted in previous studies on the effect of variations in the composition of the culture medium on the production of the enzyme amylase. This study uses local isolate amylolytic bacteria LTE-6 that have been isolated from previous studies that the enzyme is an amylase-producing bacteria. Variations in…
date: 2013-04-01
status: published
author list: authors
type: EPrint
type: OtherEPrint
is part of: repository
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http://digilib.unila.ac.id/id/document/442132
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FAKULTAS MIPA
type: Organization

0517011047 Okta Riyani
type: Person
given name: 0517011047
family name: Okta Riyani

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