?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=ISOLASI%2C+KARAKTERISASI%2C+DAN+UJI+AKTIVITAS+ANTIOKSIDAN%0D%0ASENYAWA+ASAM+KLOROGENAT+DARI+BIJI+KOPI+HIJAU+ROBUSTA+%0D%0A(Coffea+canephora+Pierre+ex+A.+Froehner)%0D%0A&rft.creator=Valentino+Budi+Pratama%2C+1517011052&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.description=Telah+diisolasi+senyawa+asam+klorogenat+dan+kafein+hidrat+dari+biji+kopi+hijau+%0D%0Arobusta+(Coffea+canephora+Pierre+ex+A.+Froehner)+yang+diambil+dari+Kecamatan+%0D%0APadang+Cermin%2C+Kabupaten+Pesawaran%2C+Provinsi+Lampung.+Tujuan+penelitian+ini+%0D%0Auntuk+mendapatkan+senyawa+asam+klorogenat+yang+memiliki+aktivitas+sebagai+%0D%0Aantioksidan.+Isolasi+asam+klorogenat+dilakukan+dengan+maserasi+menggunakan+%0D%0Ametanol+selama+3x24+jam%2C+kemudian+ekstrak+dipartisi+menggunakan+n-heksana+dan+%0D%0Aetilasetat%2C+dilanjutkan+dengan+pemurnian+menggunakan+kromatografi+kolom+dan+%0D%0Apenentuan+konsentrasi+menggunakan+HPLC+(High+performance+liquid+%0D%0Achromatography).+Karakterisasi+senyawa+dilakukan+secara+spektroskopi+UV-Vis+%0D%0Adan+inframerah%2C+serta+uji+aktivitas+antioksidan+dilakukan+dengan+menggunakan+%0D%0Ametode+DPPH+(1%2C1-difenil-2-pikrilhidrazil).+Hasil+isolasi+diperoleh+fraksi+yang+%0D%0Abelum+murni+dengan+konsentrasi+asam+klorogenat+sebesar+210%2C36+mg%2FL+dan+serbuk+%0D%0Aputih+hidrat+sebanyak+125+mg+dengan+titik+leleh+sebesar+222-224oC%2C+memiliki+%0D%0Aspektrum+UV-Vis%2C+inframerah+dan+nilai+Rf+kromatogram+lapis+tipis+yang+masing%EF%BF%BDmasing+sesuai+dengan+standar+asam+klorogenat+dan+kafein+hidrat.+Pada+uji+aktivitas+%0D%0Aantioksidan%2C+fraksi+asam+klorogenat+memiliki+aktivitas+antioksidan+yang+kuat+%0D%0Adengan+nilai+IC50+sebesar+6%2C6601+mg%2FL+dan+serbuk+kafein+hidrat+yang+kurang+aktif+%0D%0Asebagai+antioksidan+dengan+nilai+IC50+sebesar+667%2C8132+mg%2FL.%0D%0AKata+kunci%3A+aktivitas+antioksidan%2C+asam+klorogenat%2C+biji+kopi+hijau+robusta%2C+kafein+%0D%0Ahidrat.+%0D%0A%0D%0AChlorogenic+acid+and+caffeine+hydrate+compounds+have+been+isolated+from+robusta+%0D%0Agreen+beans+coffee+(Coffea+canephora+Pierre+ex+A.+Froehner)+taken+from+Padang+%0D%0ACermin+District%2C+Pesawaran+Regency%2C+Lampung+Province.+The+purpose+of+this+study+%0D%0Awas+to+obtain+chlorogenic+acid+compounds+which+have+antioxidant+activity.+%0D%0AChlorogenic+acid+isolation+was+carried+out+by+maceration+using+methanol+for+3x24+%0D%0Ahours%2C+then+the+extract+was+partitioned+using+n-hexane+and+ethylacetate%2C+followed+%0D%0Aby+purification+using+column+chromatography+and+determination+of+concentration+%0D%0Ausing+HPLC+(High+performance+liquid+chromatography).+Characterization+of+%0D%0Acompounds+was+carried+out+by+UV-Vis+and+infrared+spectroscopy%2C+and+the+%0D%0Aantioxidant+activity+test+was+carried+out+using+the+DPPH+(1%2C1-diphenyl-2-%0D%0Apikrilhidrazil)+method.+The+isolated+results+obtained+by+the+pure+fraction+with+%0D%0Achlorogenic+acid+concentration+of+210.36+mg+%2F+L+and+white+powder+hydrate+as+much+%0D%0Aas+125+mg+with+a+melting+point+of+222-224oC%2C+have+a+UV-Vis+spectrum%2C+infrared+%0D%0Aand+thin+layer+chromatogram+Rf+values+each+of+which+corresponds+to+standard+%0D%0Achlorogenic+acid+and+caffeine+hydrate.+In+the+antioxidant+activity+test%2C+the+%0D%0Achlorogenic+acid+fraction+had+a+strong+antioxidant+activity+with+an+IC50+value+of+%0D%0A6.6601+mg%2FL+and+caffeine+hydrate+powder+which+was+less+active+as+an+antioxidant+%0D%0Awith+an+IC50+value+of+667.8132+mg%2FL.%0D%0AKeywords%3A+antioxidant+activity%2C+chlorogenic+acid%2C+robusta+green+beans+coffee%2C+%0D%0Acaffeine+hydrate.%0D%0A%0D%0A&rft.publisher=Fakultas+Matematika+dan+Ilmu+Pengetahuan+Alam&rft.date=2019&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F57176%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F57176%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F57176%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++Valentino+Budi+Pratama%2C+1517011052++(2019)+ISOLASI%2C+KARAKTERISASI%2C+DAN+UJI+AKTIVITAS+ANTIOKSIDAN+SENYAWA+ASAM+KLOROGENAT+DARI+BIJI+KOPI+HIJAU+ROBUSTA+(Coffea+canephora+Pierre+ex+A.+Froehner).++Fakultas+Matematika+dan+Ilmu+Pengetahuan+Alam%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F57176%2F