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<http://digilib.unila.ac.id/id/eprint/57307> <http://purl.org/dc/terms/title> "PENGARUH PEMBERIAN MIKROKAPSUL PROBIOTIK DENGAN \r\nDOSIS BERBEDA TERHADAP TINGKAT IMUNITAS IKAN KERAPU \r\nMACAN Epinephelus fuscoguttatus (Forsskal, 1775)\r\n"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/eprint/57307> <http://purl.org/ontology/bibo/abstract> "Vibriosis infected tiger grouper fish while the immunity system relatively low. \r\nThe fish immune system can be improved by additioning probiotics into the fish \r\nbody. However, probiotics that given to fish are not fully digested into the \r\ndigestive tract as a whole due to environmental influences that can damage the \r\nstructure of probiotic cells. In overcoming this, we need a technique called \r\nmicrocapsules. This study aims to determine the effect of probiotic microcapsules, \r\nthis study use Bacillus sp. D2.2, to the immunity level of tiger grouper that\r\ninfected with V. alginolyticus. This research was conducted in a completely \r\nrandomized design (CRD) with 5 treatments and 3 replications. The parameters \r\nobserved included bacterial viability test, total erythrocytes, total leukocytes, \r\ndifferential leukocytes, antibody titer, clinical symptoms, survival rate, and \r\nrelative percent survival (RPS). The results showed that the immunity response of \r\ntiger grouper fish increased significantly after probiotic microcapsules were given.\r\nKeywords: Total Leukocytes, Bacillus sp. D2.2, Vibrio alginolyticus, Viability \r\nVibriosis dapat menyerang ikan disebabkan oleh sistem imunitas ikan kerapu \r\nmacan yang rendah. Sistem imunitas ikan dapat ditingkatkan dengan cara \r\npemberian probiotik ke dalam tubuh. Namun, probiotik yang diberikan kepada \r\nikan umumnya tidak tercerna sepenuhnya ke dalam tubuh secara utuh dikarenakan \r\nadanya pengaruh lingkungan yang mampu merusak struktur sel probiotik. Dalam \r\nmenanggulangi hal tersebut, dibutuhkan suatu teknik yang bernama mikrokapsul. \r\nPenelitian ini bertujuan untuk mempelajari pengaruh pemberian mikrokapsul \r\nprobiotik, probiotik yang digunakan adalah Bacillus sp. D2.2 dan menentukan \r\ndosis yang tepat terhadap tingkat imunitas ikan kerapu macan yang di infeksi \r\nbakteri V. alginolyticus. Penelitian ini dilakukan secara Rancangan Acak Lengkap \r\n(RAL) dengan 5 perlakuan dan 3 ulangan. Parameter yang diamati meliputi uji \r\nviabilitas bakteri, total eritrosit, total leukosit, differensial leukosit, titer antibodi, \r\ngejala klinis, sintasan, dan relative percent survival (RPS). Hasil penelitian \r\nmenunjukkan bahwa parameter respon imun ikan kerapu macan meningkat secara \r\nsignifikan pada perlakuan pemberian mikrokapsul\r\nKata kunci: Total Leukosit, Bacillus sp. D2.2, Vibrio alginolyticus, Viabilitas \r\n"^^<http://www.w3.org/2001/XMLSchema#string> .
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