@misc{eprints59510,
           month = {Januari},
           title = {IDENTIFIKASI SINGLE NUCLEOTIDE POLYMORPHISM (SNP)
KODON 1034 GEN PfMDR1 PADA PENDERITA MALARIA
FALCIPARUM DI WILAYAH KERJA PUSKESMAS HANURA,
KABUPATEN PESAWARAN, PROVINSI LAMPUNG},
          author = {1518011011 Puji Indah Permatasari},
         address = {UNIVERSITAS LAMPUNG},
       publisher = {Fakultas Kedokteran },
            year = {2019},
             url = {http://digilib.unila.ac.id/59510/},
        abstract = {Latar Belakang: Plasmodium falciparum merupakan salah satu jenis
Plasmodium yang dapat menyebabkan penyakit malaria. Plasmodium falciparum
yang resisten terhadap obat anti malaria disebabkan oleh adanya mutasi genetik.
Adanya Single Nucleotide Polymorphism pada kodon 1034 gen Plasmodium
Falciparum Multidrug Resistance 1 (Pfmdr1) dapat menjadi penanda genetik
resistensi obat klorokuin terhadap Plasmodium falciparum. Pemeriksaan yang
dilakukan berbasis biologi molekuler sudah banyak diteliti untuk mendeteksi
polimorfisme gen melalui Polymerase Chain Reaction (PCR) dan dianalisa secara
sekuensing.
Metode: Jenis penelitian ini menggunakan rancangan penelitian survey dan
bersifat deskriptif. Sampel penelitian diperoleh dari Bahan Biologi Tersimpan
(BBT) sebanyak 22 sampel. Penelitian ini dilakukan dengan menggunakan
metode PCR yang dianalisis dengan metode sekuensing untuk mendeteksi
polimorfisme pada kodon 1034 gen Pfmdr1.
Hasil: Terdapat 22 sampel yang telah dilakukan nested PCR, kemudian 12
sampel dilanjutkan sekuensing dengan hasil kodon 1034 gen Pfmdr1 pada seluruh
sampel adalah bersifat wild-type.
Kesimpulan: Tidak terdapat Single Nucleotide Polymorphism kodon 1034 gen
Plasmodium Falciparum Multidrug Resistance 1 (Pfmdr1).
Kata Kunci: Kodon, Plasmodium Falciparum Multidrug Resistance 1 (Pfmdr1),
Polymerase Chain Reaction (PCR)

Background: Plasmodium falciparum is one of Plasmodium which can cause
malaria. Plasmodium falciparum that is resistant to anti-malaria drugs caused by
genetic mutations. The presence of a single nucleotide polymorphism in codon
1034 of the Plasmodium Falciparum Multidrug gene Resistance 1 (Pfmdr1) can
be a genetic marker of chloroquine drug resistance to Plasmodium falciparum.
Examinations carried out based on molecular biology have been widely
investigated to detect gene polymorphisms through Polymerase Chain Reaction
(PCR) and analyzed sequentially.
Method: This research used a survey research design with descriptive method.
Sample obtained from 22 stored Archived Biological Materials (ABM). The
examination was carried out by using the PCR method and analyzed by
sequencing to detect the polymorphism of codon 1034 gene Pfmdr1..
Result: There were 22 samples that had been carried out nested PCR, then 12
samples were continued sequencing with the result of codon 1034 Pfmdr1 gene in
all samples were wild-type.
Conclusion: There are no Single Nucleotide Polymorphism codon 1034
Plasmodium Falciparum Multidrug Resistance 1 (Pfmdr1).
Keyword: Codon, Plasmodium Falciparum Multidrug Resistance 1 (Pfmdr1),
Polymerase Chain Reaction (PCR).}
}