?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=IDENTIFIKASI+SINGLE+NUCLEOTIDE+POLYMORPHISM+(SNP)%0D%0AKODON+1034+GEN+PfMDR1+PADA+PENDERITA+MALARIA%0D%0AFALCIPARUM+DI+WILAYAH+KERJA+PUSKESMAS+HANURA%2C%0D%0AKABUPATEN+PESAWARAN%2C+PROVINSI+LAMPUNG&rft.creator=Puji+Indah+Permatasari%2C+1518011011&rft.subject=610+Ilmu+kedokteran%2C+ilmu+pengobatan+dan+ilmu+kesehatan&rft.description=Latar+Belakang%3A+Plasmodium+falciparum+merupakan+salah+satu+jenis%0D%0APlasmodium+yang+dapat+menyebabkan+penyakit+malaria.+Plasmodium+falciparum%0D%0Ayang+resisten+terhadap+obat+anti+malaria+disebabkan+oleh+adanya+mutasi+genetik.%0D%0AAdanya+Single+Nucleotide+Polymorphism+pada+kodon+1034+gen+Plasmodium%0D%0AFalciparum+Multidrug+Resistance+1+(Pfmdr1)+dapat+menjadi+penanda+genetik%0D%0Aresistensi+obat+klorokuin+terhadap+Plasmodium+falciparum.+Pemeriksaan+yang%0D%0Adilakukan+berbasis+biologi+molekuler+sudah+banyak+diteliti+untuk+mendeteksi%0D%0Apolimorfisme+gen+melalui+Polymerase+Chain+Reaction+(PCR)+dan+dianalisa+secara%0D%0Asekuensing.%0D%0AMetode%3A+Jenis+penelitian+ini+menggunakan+rancangan+penelitian+survey+dan%0D%0Abersifat+deskriptif.+Sampel+penelitian+diperoleh+dari+Bahan+Biologi+Tersimpan%0D%0A(BBT)+sebanyak+22+sampel.+Penelitian+ini+dilakukan+dengan+menggunakan%0D%0Ametode+PCR+yang+dianalisis+dengan+metode+sekuensing+untuk+mendeteksi%0D%0Apolimorfisme+pada+kodon+1034+gen+Pfmdr1.%0D%0AHasil%3A+Terdapat+22+sampel+yang+telah+dilakukan+nested+PCR%2C+kemudian+12%0D%0Asampel+dilanjutkan+sekuensing+dengan+hasil+kodon+1034+gen+Pfmdr1+pada+seluruh%0D%0Asampel+adalah+bersifat+wild-type.%0D%0AKesimpulan%3A+Tidak+terdapat+Single+Nucleotide+Polymorphism+kodon+1034+gen%0D%0APlasmodium+Falciparum+Multidrug+Resistance+1+(Pfmdr1).%0D%0AKata+Kunci%3A+Kodon%2C+Plasmodium+Falciparum+Multidrug+Resistance+1+(Pfmdr1)%2C%0D%0APolymerase+Chain+Reaction+(PCR)%0D%0A%0D%0ABackground%3A+Plasmodium+falciparum+is+one+of+Plasmodium+which+can+cause%0D%0Amalaria.+Plasmodium+falciparum+that+is+resistant+to+anti-malaria+drugs+caused+by%0D%0Agenetic+mutations.+The+presence+of+a+single+nucleotide+polymorphism+in+codon%0D%0A1034+of+the+Plasmodium+Falciparum+Multidrug+gene+Resistance+1+(Pfmdr1)+can%0D%0Abe+a+genetic+marker+of+chloroquine+drug+resistance+to+Plasmodium+falciparum.%0D%0AExaminations+carried+out+based+on+molecular+biology+have+been+widely%0D%0Ainvestigated+to+detect+gene+polymorphisms+through+Polymerase+Chain+Reaction%0D%0A(PCR)+and+analyzed+sequentially.%0D%0AMethod%3A+This+research+used+a+survey+research+design+with+descriptive+method.%0D%0ASample+obtained+from+22+stored+Archived+Biological+Materials+(ABM).+The%0D%0Aexamination+was+carried+out+by+using+the+PCR+method+and+analyzed+by%0D%0Asequencing+to+detect+the+polymorphism+of+codon+1034+gene+Pfmdr1..%0D%0AResult%3A+There+were+22+samples+that+had+been+carried+out+nested+PCR%2C+then+12%0D%0Asamples+were+continued+sequencing+with+the+result+of+codon+1034+Pfmdr1+gene+in%0D%0Aall+samples+were+wild-type.%0D%0AConclusion%3A+There+are+no+Single+Nucleotide+Polymorphism+codon+1034%0D%0APlasmodium+Falciparum+Multidrug+Resistance+1+(Pfmdr1).%0D%0AKeyword%3A+Codon%2C+Plasmodium+Falciparum+Multidrug+Resistance+1+(Pfmdr1)%2C%0D%0APolymerase+Chain+Reaction+(PCR).&rft.publisher=Fakultas+Kedokteran+&rft.date=2019-01-23&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F59510%2F1%2FAbstrak%2520%2528Abstract%2529.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F59510%2F2%2FSkripsi%2520Full.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F59510%2F3%2FSkripsi%2520Tanpa%2520Bab%2520Pembahasan.pdf&rft.identifier=++Puji+Indah+Permatasari%2C+1518011011++(2019)+IDENTIFIKASI+SINGLE+NUCLEOTIDE+POLYMORPHISM+(SNP)+KODON+1034+GEN+PfMDR1+PADA+PENDERITA+MALARIA+FALCIPARUM+DI+WILAYAH+KERJA+PUSKESMAS+HANURA%2C+KABUPATEN+PESAWARAN%2C+PROVINSI+LAMPUNG.++Fakultas+Kedokteran+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F59510%2F