?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=STUDI+STABILITAS+LIPASE+DARI+BAKTERI+LOKAL+ISOLAT+LKM+A3+%0D%0ADALAM+BERBAGAI+PELARUT+ORGANIK+DAN+PENENTUAN%0D%0AAKTIVITAS+ESTERIFIKASI+%0D%0A&rft.creator=AIGA+SHEIRA+RAIT%2C+1757011007&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.description=+%0D%0A%0D%0A+%0D%0ALipase+merupakan+biokatalis+yang+dapat+menghidrolisis+lemak+menghasilkan%0D%0Aasam+lemak+dan+gliserol.+Lipase+juga+memiliki+kemampuan+untuk+mengkatalisis%0D%0Areaksi+sintesis+organik+seperti+esterifikasi%2C+transesterifikasi%2C+alkoholisis+dan+lainlain.%0D%0APenelitian%0D%0Aini%0D%0Abertujuan%0D%0Auntuk%0D%0Amempelajari%0D%0Akestabilan%0D%0Alipase%0D%0Adari%0D%0Abakteri%0D%0Alokal%0D%0A%0D%0Aisolat%0D%0A%0D%0ALKM+A3+dalam+berbagai+pelarut+organik+dan+mengetahui+kemampuannya%0D%0Adalam+mengkatalisis+reaksi+esterifikasi.+Tahapan+yang+dilakukan+meliputi%0D%0Aproduksi+dan+pemurnian+enzim%2C+uji+stabilitas+lipase+dalam+berbagai+pelarut+organik%0D%0Adan+uji+reaksi+esterifikasi.+Hasil+menunjukkan+bahwa+produksi+enzim+pada+kondisi%0D%0Aoptimum+pH%2C+suhu+30%0D%0Ao%0D%0AC+dan+penambahan+induser+minyak+kelapa+sawit+10%25%0D%0Amemberikan+nilai+aktivitas+spesifik+1342%2C63+U+mg%0D%0A-1%0D%0A.+Tahap+pemurnian+dengan%0D%0Aamonium+sulfat+hingga+dialisis+dapat+meningkatkan+kemurnian+lipase+menjadi%0D%0A3%2C17+kali+dibandingkan+esktrak+kasar+dengan+aktivitas+spesifik+hasil+dialisis%0D%0Asebesar+4197%2C66+U+mg%0D%0A-1%0D%0A.+Enzim+lipase+dari+bakteri+lokal+isolat+LKM+A3+juga%0D%0Amenunjukkan+kestabilan+relatif+lebih+besar+dalam+pelarut+heksana+dibandingkan%0D%0Apelarut+organik+lainnya%2C+dengan+nilai+aktivitas+sisa+sebesar+52%2C25%25.+Uji%0D%0Akemampuan+lipase+sebagai+katalis+dalam+reaksi+esterifikasi+juga+memberikan+hasil%0D%0Apositif+pada+analisis+FTIR+yang+ditandai+dengan+terbentuknya+gugus+karbonil+C%3DO%0D%0Apada+panjang+gelombang+1744+cm%0D%0A-1%0D%0A.+Berdasarkan+hasil+tersebut%2C+dapat%0D%0Adisimpulkan+bahwa+bakteri+lokal+isolat+LKM+A3+mampu+menghasilkan+lipase%0D%0Adengan+tingkat+kemurnian+relatif+lebih+tinggi+dan+memiliki+kestabilan+relatif+lebih%0D%0Abaik+dalam+pelarut+heksana.+Lipase+dari+bakteri+ini+juga+dapat+digunakan+sebagai%0D%0Akatalis+dalam+reaksi+esterifikasi.%0D%0A%0D%0AKata+kunci%3A+esterifikasi%2C+lipase%2C+pelarut+organik%2C+pemurnian+enzim%0D%0A+%0D%0A3%0D%0A+%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A+%0D%0AABSTRACT%0D%0A+%0D%0A%0D%0ASTUDY+OF+LIPASE+STABILITY+FROM+LOCAL+BACTERIA+OF+LKM+A3+%0D%0AISOLATE+IN+VARIOUS+ORGANIC+SOLVENTS+AND+DETERMINATION%0D%0AOF+ESTERIFICATION+ACTIVITY+%0D%0A%0D%0A%0D%0ABy%0D%0A%0D%0A+%0D%0AAIGA+SHEIRA+RAIT%0D%0A+%0D%0A%0D%0A+%0D%0ALipase+is+a+biocatalyst+that+can+hydrolyze+fats+to+produce+fatty+acids+and+glycerol.%0D%0ALipase+also+can+catalyze+organic+synthesis+reactions+such+as+esterification%2C%0D%0Atransesterification%2C+alcoholysis+and+others.+The+purpose+of+this+research+are+to%0D%0Astudy+lipase+stability+from+local+bacteria+of+LKM+A3+isolate+in+various+organic%0D%0Asolvents+and+to+determine+its+ability+to+catalyze+esterification+reactions.+The+steps%0D%0Aare+enzyme+production+and+purification%2C+lipase+stability+test+in+various+organic%0D%0Asolvents+and+esterification+reaction+test.+The+results+showed+that+the+enzyme%0D%0Aproduction+at+optimum+conditions+of+pH+5%2C+temperature+30%0D%0Ao%0D%0AC+and+the+addition+of%0D%0A10%25+palm+oil+inducer+gave+a+specific+activity+value+of+1342.63+U+mg%0D%0A-1%0D%0A.+The%0D%0Apurification+step+with+ammonium+sulfate+until+dialysis+can+increase+the+purity+of%0D%0Alipase+to+3.17+times+compared+to+crude+extract+with+specific+activity+from+dialysis%0D%0Aof+4197.66+U+mg%0D%0A-1%0D%0A.+The+lipase+enzyme+from+local+bacteria+isolates+LKM+A3+also%0D%0Ashowed+greater+relative+stability+in+hexane+solvents+than+other+organic+solvents%2C%0D%0Awith+a+residual+activity+value+of+52.25%25.+The+ability+test+of+lipase+as+a+catalyst+in%0D%0Athe+esterification+reaction+also+gave+positive+results+on+the+FTIR+analysis+which%0D%0Awas+indicated+by+the+formation+of+a+C%3DO+carbonyl+group+at+a+wavelength+of+1744%0D%0Acm%0D%0A-1%0D%0A.+Based+on+these+results%2C+it+can+be+concluded+that+the+local+bacteria+of+LKM%0D%0AA3+isolate+were+able+to+produce+lipase+with+a+relatively+higher+level+of+purity+and%0D%0Arelatively+better+stability+in+hexane+solvent.+Lipase+from+this+bacteria+can+also+be%0D%0Aused+as+a+catalyst+in+the+esterification+reaction.%0D%0A%0D%0AKey+words%3A+esterification%2C+lipase%2C+organic+solvents%2C+enzyme+purification%0D%0A+&rft.publisher=FAKULTAS+MIPA&rft.date=2021&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F62008%2F1%2FABSTRAK%2520-%2520Aiga%2520Sheira.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F62008%2F2%2FSKRIPSI%2520FULL%2520-%2520Aiga%2520Sheira.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F62008%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN%2520-%2520Aiga%2520Sheira.pdf&rft.identifier=++AIGA+S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