?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=KERAGAMAN+MORFOLOGI+DAN+GENOTIPIK+BERDASARKAN%0D%0AMARKA+RAPD+SERTA+PERBANYAKAN+VEGETATIF+DENGAN%0D%0ASTEK+PADA+SEJUMLAH+GENOTIPE+KOPI+ROBUSTA%0D%0A%0D%0A(Coffea+canephora+Pierre+ex+A+Froehner)%0D%0A%0D%0ADI+LAMPUNG&rft.creator=SRI+RAMADIANA%2C+1534171002&rft.subject=600+Teknologi+(ilmu+terapan)&rft.subject=630+Pertanian+dan+teknologi+yang+berkaitan&rft.description=Kopi+Robusta+(Coffea+canephora+Pierre+ex+A+Froehner)+yang+dibudidayakan+di%0D%0ALampung+memiliki+produktivitas+yang+tertinggi+kedua+di+Indonesia+setelah+Sumatera%0D%0ASelatan%2C+dengan+hasil+sebesar+831kg%2Fha%2Fth+dan+lebih+besar+dari+rata-rata+produktivitas%0D%0Akopi+Indonesia+(716+kg%2Fha%2Fth).+Akan+tetapi+produktivitas+kopi+Lampung+ini+jauh+lebih%0D%0Arendah+dibandingkan+produktivitas+kopi+negara+Brazil+(3.346+kg%2Fha%2Fth)+dan+Vietnam%0D%0A(1.781+kg%2Fha%2Fth).+Penyebab+utama+rendahnya+produktivitas+kopi+di+Lampung+adalah%0D%0Aterkait+dengan+teknik+budidaya%2C+penyediaan+bibit+unggul+dan+program+pemuliaan+kopi%0D%0Ayang+belum+terarah.+Peningkatan+produktivitas+kopi+di+Lampung+dapat+dilakukan%0D%0Adengan%2C+pertama%2C+mengidentifikasi+karakter+morfologi+dan+genetiknya+untuk%0D%0Amengetahui+tingkat+kekerabatannya.+Selanjutnya%2C+kedua%2C+berdasarkan+tingkat%0D%0Akekerabatannya%2C+dapat+dipilih+genotipe-genotipe+untuk+ditanam+bersama-sama+dalam%0D%0Asistem+budidaya+poliklonal+untuk+mendapatkan+produktivitas+yang+tinggi.+Sistem%0D%0Abudidaya+poliklonal+dari+klon+kopi+unggul+Lampung+harus+didukung+oleh+ketersediaan%0D%0Abibit+dalam+jumlah+banyak+dan+bermutu.+Penyediaan+bibit+dalam+jumlah+besar+dapat%0D%0Adilakukan+dengan+menggunakan+perbanyakan+vegetatif+melalui+stek+cabang+ortotrop.%0D%0APenelitian+ini+bertujuan+untuk+a)+mengidentifikasi+dan+mengkarakterisasi+morfologi+kopi%0D%0Arobusta+(Coffea+canephora)+unggul+Lampung%3B+b)+menentukan+keragaman+genetik+klon%0D%0Akopi+Robusta+unggul+lokal+dan+nasional+di+Lampung+menggunakan+marka+RAPD%3B+c)%0D%0Amendapatkan+teknik+perbanyakan+klonal+kopi+Robusta+yang+efisien+melalui+penggunaan%0D%0Azat+pengatur+tumbuh+IBA+dan+NAA.+Hasil+penelitian+menunjukkan+bahwa+terdapat%0D%0Akesamaan+karakter+morfologi+pada+beberapa+genotipe+tertentu+dari+15+genotipe+yang%0D%0Adievaluasi.+Tugu+Sari+memiliki+panjang+daun+yang+hampir+sama+dengan+genotipe+dari%0D%0AKabupaten+Tanggamus+seperti+Tugino%2C+Wanto%2C+dan+Biyadi.+Tiga+genotipe+dari%0D%0ATanggamus+(Tugino%2C+Wanto+dan+Biyadi)+memiliki+diameter+batang+yang+lebih+lebar%0D%0Adibandingkan+genotipe+unggul+nasional.+Dilihat+dari+karakteristik+biji%2C+genotipe+kopi%0D%0Adari+Tanggamus+secara+umum+menghasilkan+biji+yang+lebih+panjang%2C+lebih+tebal+dan%0D%0Alebih+berat+dibandingkan+dengan+biji+yang+dihasilkan+oleh+genotipe+dari+Kabupaten%0D%0ALampung+Barat.+Tingkat+kemurnian+DNA+hasil+ekstraksi+menggunakan+nitrogen+cair%0D%0Alebih+tinggi+daripada+tingkat+kemurnian+DNA+hasil+ekstraksi+menggunakan+teknik%0D%0Aperendaman+dengan+etanol+absolut+96%25.+Terdapat+11+primer+terseleksi+yang+digunakan%0D%0Adalam+reaksi+PCR+yaitu+OPE-18%2C+OPI-07+OPN16%2C+OPG-03%2C+OPR1%2C+OPL18%2C+OPM13%2C%0D%0AOPN18%2C+OPO15%2C+OPO12+dan+OPO5.+Kondisi+PCR+yang+optimal+pada+penelitian+ini%0D%0Ayaitu+satu+siklus+denaturasi+awal+pada+suhu+940C+selama+3+menit+diikuti+30+siklus+yang%0D%0Aterdiri+dari+denaturasi+pada+suhu+940C+selama+1menit%2C+annealing+pada+suhu+370%0D%0AC%0D%0Aselama+1+menit%2C+dan+ekstensi+pada+720C+selama+2+menit%2C+lalu+diikuti+dengan+ekstensi%0D%0A%0D%0Axxiii%0D%0A%0D%0Aakhir+pada+720C+selama+10+menit.+Reaksi+dilakukan+pada+volume+25+%CE%BCl+dalam+tabung%0D%0Areaksi+200+%CE%BCl+yang+berisi+50+ng+templat+DNA%2C+12%2C5+%CE%BCl+kit+PCR+(Qiagen)%2C+2+%CE%BCl+primer%2C%0D%0Adan+sisanya+adalah+akuades+steril.+Analisis+RAPD+menggunakan+11+primer%0D%0Amenunjukkan+adanya+variasi+jumlah+pita+polimorfik+dan+ukuran+fragmen+DNA+yang%0D%0Ateramplifikasi.+Kemiripan+genetik+antargenotipe+Robusta+yang+digunakan+dalam%0D%0Apenelitian+ini+berkisar+antara+25%2C6%25+-+93%25.+Berdasarkan+jarak+genetiknya%2C+genotipe%0D%0ABlirik%2C+Imam+1%2C+BP+913%2C+BP+203%2C+dan+BP+534+berpotensi+untuk+dijadikan+tetua%0D%0Apersilangan+untuk+mendapatkan+efek+heterosis.+Pemberian+auksin+IBA+1000+ppm%0D%0Amenyebabkan+peningkatan+persentase+stek+berakar%2C+jumlah+akar%2C+panjang+akar%2C+dan%0D%0Ajumlah+tunas.+Pemberian+IBA+1000+ppm+pada+genotipe+Komari+dan+Tugino%0D%0Amenghasilkan+tunas+yang+lebih+panjang+daripada+genotipe+lainnya+yang+dicoba.+yaitu%0D%0A8%2C72-9%2C08+cm%3B+(2)+Jenis+auksin%2C+yaitu+IBA%2C+NAA+atau+kombinasi+(IBA%2BNAA)+tidak%0D%0Aberpengaruh+terhadap+persentase+stek+berakar%2C+jumlah+akar%2C+panjang+akar%2C+dan+panjang%0D%0Atunas%2C+tetapi+berpengaruh+terhadap+bobot+kering+akar.+Perlakuan+(IBA%2BNAA)%0D%0Amenghasilkan+bobot+kering+akar+yang+lebih+tinggi+dibandingkan+perlakuan+IBA+atau%0D%0ANAA+saja.+Kombinasi+(IBA%2BNAA)+2000+ppm+menghasilkan+bobot+kering+akar%0D%0Atertinggi.+Konsentrasi+auksin+1000+dan+2000+ppm+menyebabkan+peningkatan%0D%0Apembentukan+dan+pertumbuhan+tunas+dengan+hasil+terbaik+pada+2000+ppm.+The+productivity+of+Robusta+coffee+(Coffea+canephora+Pierre+ex+A+Froehner)+cultivated%0D%0Ain+Lampung+(831+kg%2Fha%2Fyr)+came+second+to+that+of+South+Sumatera%2C+being+higher+than%0D%0Athe+average+coffee+productivity+in+Indonesia+(716+kg%2Fha%2Fyr).+However%2C+coffee%0D%0Aproductivity+in+Lampung+is+lower+than+that+of+Brazil+(3%2C346+kg%2Fha%2Fyr)+and+Vietnam%0D%0A(1%2C781+kg%2Fha%2Fyr).+This+low+productivity+is+related+to+cultivation+techniques%2C+seed+quality%0D%0Aand+coffee+breeding+programs.+To+increase+coffee+productivity+is+firstly+to+determine%0D%0Agenetic+relatedness+among+coffee+clones+in+Lampung+based+on+genetic+and%0D%0Amorphological+characters%2C+and+secondly+to+select+clones+to+be+used+in+polyclonal%0D%0Acultivation+which+results+in+high+productivity.+The+polyclonal+cultivation+must+be%0D%0Asupported+by+the+availability+of+good+quality+planting+materials+such+as+cuttings+from%0D%0Aorthotropic+branches.+This+study+aimed+to+a)+conduct+morphological+characterization+of%0D%0Asuperior+Robusta+coffee+(Coffea+canephora)+in+Lampung%3B+b)+determine+the+genetic%0D%0Adiversity+among+local+and+national+superior+clones+of+Robusta+coffee+cultivated+in%0D%0ALampung+based+on+RAPD+markers%2C+c)+obtain+an+efficient+clonal+propagation+of+Robusta%0D%0Acoffee+by+cuttings+using+plant+growth+regulators+IBA+and+NAA.+It+was+found+that%0D%0Acertain+genotypes+showed+similar+morphological+characters+among+15+Robusta+coffee%0D%0Agenotypes.+Tugu+Sari+was+found+to+have+leaf+length+that+was+almost+the+same+as+that+of%0D%0Agenotypes+from+Tanggamus+Regency+such+as+Tugino%2C+Wanto%2C+and+Biyadi.+Three%0D%0Agenotypes+from+Tanggamus+(Tugino%2C+Wanto+and+Biyadi)+had+wider+stem+diameters+than%0D%0Athe+national+superior+genotypes.+Judging+from+the+characteristics+of+the+beans%2C+coffee%0D%0Agenotypes+from+Tanggamus+generally+produced+coffee+beans+that+were+longer%2C+thicker%0D%0Aand+heavier+than+those+from+West+Lampung+Regency.+The+purity+of+DNA+extracted%0D%0Ausing+liquid+nitrogen+was+better+than+DNA+extracted+using+the+immersion+technique%0D%0Awith+96%25+absolute+ethanol.+There+were+11+selected+primers+used+in+PCR+reaction%2C%0D%0Anamely+OPE-18%2C+OPI-07+OPN16%2C+OPG-03%2C+OPR1%2C+OPL18%2C+OPM13%2C+OPN18%2C+OPO15%2C%0D%0AOPO12+and+OPO5.+The+optimal+PCR+condition+was+as+follows%3A+one+cycle+of+initial%0D%0Adenaturation+at+94%C2%B0C+for+3+minute+followed+by+30+cycles+of+denaturation+at+94%C2%B0C+for+1%0D%0Aminute%2C+annealing+at+37%C2%B0C+for+1+minute%2C+and+extension+at+72%C2%B0C+for+2+minutes.+The+final%0D%0Aextension+was+done+at+72%C2%B0C+for+10+minutes.+The+PCR+reaction+was+done+in+a+volume+of%0D%0A25+%CE%BCl+in+a+200-%CE%BCl+microtube+containing+12.5+%CE%BCl+of+PCR+kit+(QIAGEN)%2C+50+ng+of+DNA%0D%0Atemplate+and+2+%CE%BCl+of+a+random+primer.+DNA+analysis+using+11+RAPD+markers+showed%0D%0Avariations+in+the+number+of+polymorphic+bands+and+the+size+of+amplified+products.+The%0D%0Agenetic+similarity+among+the+Robusta+genotypes+used+in+this+study+ranged+from+25.6%25+-%0D%0A93%25.+Based+on+their+genetic+distance%2C+the+genotypes+of+Blirik%2C+Imam+1%2C+BP+913%2C+BP+203%2C%0D%0Aand+BP+534+had+the+potential+to+be+used+as+parents+in+a+breeding+program+to+get%0D%0A%0D%0Axxv%0D%0A%0D%0Aheterosis+effects.+The+application+of+1000+ppm+IBA+resulted+in+increased+rooting%0D%0Apercentage%2C+root+number%2C+root+length+and+shoot+number.+Application+of+1000+ppm+of%0D%0AIBA+to+the+Komari+and+Tugino+genotypes+resulted+in+the+highest+shoot+length+(8.72-9.08%0D%0Acm).+Types+of+auxins+(IBA%2C+NAA+or+IBA%2BNAA)+had+no+effects+on+root+formation+and%0D%0Ashoot+growth%2C+but+had+effects+on+root+dry+weight.+The+treatment+of+(IBA%2BNAA)+led+to%0D%0Ahigher+root+dry+weight+than+that+of+IBA+or+NAA+alone.+Auxin+concentrations+of+1000%0D%0Aand+2000+ppm+led+to+an+increase+in+the+formation+and+growth+of+shoots%2C+the+best%0D%0Aconcentration+being+2000+ppm.&rft.publisher=FAKULTAS+PERTANIAN&rft.date=2021&rft.type=Disertasi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F62163%2F1%2FABSTRAK%2520-%2520Atalah%2520Printing_10.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F62163%2F2%2FDISERTASI%2520FULL%2520-%2520Atalah%2520Printing.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F62163%2F3%2FDISERTASI%2520TANPA%2520PEMBAHASAN%2520-%2520Atalah%2520Printing_3.pdf&rft.identifier=++SRI+RAMADIANA%2C+1534171002++(2021)+KERAGAMAN+MORFOLOGI+DAN+GENOTIPIK+BERDASARKAN+MARKA+RAPD+SERTA+PERBANYAKAN+VEGETATIF+DENGAN+STEK+PADA+SEJUMLAH+GENOTIPE+KOPI+ROBUSTA+(Coffea+canephora+Pierre+ex+A+Froehner)+DI+LAMPUNG.++%5BDisertasi%5D+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F62163%2F