@misc{eprints62304,
           title = {POTENSI EKSTRAK Streptomyces sp. strain i18 SEBAGAI
ANTIBIOTIK TERHADAP BAKTERI Escherichia coli},
          author = {1718011145 FARHAN DZAKI ALFAHRI},
         address = {UNIVERSITAS LAMPUNG},
       publisher = {FAKULTAS KEDOKTERAN},
            year = {2021},
             url = {http://digilib.unila.ac.id/62304/},
        abstract = {Background: Antibiotic resistant microbes (MRA) are microbes that are able to resist the
effects of antibiotics, so that these microbes continue to grow. Results of Antimicrobial
Resistant in Indonesia (AMRIN-Study) research. In 2494 individuals in Indonesia, showed
that 43\% of Escherichia coli were resistant to various types of antibiotics such as
ampicillin, cotrimoxazole and ciprofloxacin. This study shows that the problem of
antibiotic resistance also occurs in Indonesia. Escherichia coli has become a pathogen that
is widely found in food and beverages that can cause diarrhea, hemorrhagic colitis and
hemolytic uremic syndrome in humans. One source of antibiotic secondary metabolites can
be derived from the genus Streptomyces.
Objective: To determine the potential of Streptomyces sp strain i18 extract as an antibiotic
against Escherichia coli bacteria
Method: This research consisted of the phytochemical test phytochemical test the diameter
of the inhibition zone test of the well method, the Minimum Inhibitory Concentration
(MIC), Minimum Killing Rate (KBM) with the extract of Streptomyces sp strain i18 with
concentrations of 5\%, 10\%, 20\%, 40\%, 80\%, aquadest as a negative control and
ciprofloxacin as a positive control on the growth of Escherichia coli bacteria.
Result: There was no diameter of inhibition zone, MIC and MBC on Escherichia coli
bacteria by the extract of Streptomyces sp strain i18, the phytochemical test results show
that the extract of Streptomyces sp strain i18 contains secondary metabolites in the form of
saponins, triterpenoids and anthraquinone glycosides.
Conclusion: The extract of Streptomyces sp strain i18 did not have antibiotic potential
against Escherichia coli.
Keyword: Streptomyces sp strain i18, Antibiotics, Escherichia coli
Latar Belakang: Mikroba resisten antibiotik (MRA) merupakan mikroba yang mampu
melawan efek antibiotik, sehingga mikroba tersebut tetap tumbuh. Hasil penelitian
Antimicrobial Resistant in Indonesia (AMRIN-Study). Pada 2494 individu di indonesia,
menunjukan bahwa 43\% Escherichia coli resisten terhadap berbagai jenis antibiotik seperti
ampisilin, kotrimoksazol dan siprofloksasin. Penelitian ini menunjukan bahwa masalah
resistensi antibiotik juga terjadi di Indonesia. Escherichia coli sudah menjadi patogen yang
banyak terdapat pada makanan dan minuman yang dapat menyebabkan diare, kolitis
perdarahan dan hemolytic uremic syndrome pada manusia. Salah satu sumber metabolit
sekunder yang bersifat antibiotik dapat berasal dari genus Streptomyces.
Tujuan: Untuk mengetahui potensi ekstrak Streptomyces sp strain i18 sebagai antibiotik
terhadap bakteri Escherichia coli.
Metode: Penelitian ini terdiri dari ui fitokimia, uji diameter zona hambat metode sumuran,
uji Kadar Hambat Minimum (KHM), Kadar Bunuh Minimum (KBM) dengan bahan uji
ekstrak Streptomyces sp strain i18 dengan konsentrasi 5\%, 10\%, 20\%, 40\%, 80\%, aquadest
sebagai kontrol negatif dan siprofloksasin sebagai kontrol positif terhadap pertumbuhan
bakteri Escherichia coli.
Hasil: Tidak terdapat diameter zona hambat, KHM dan KBM pada bakteri Escherichia coli
oleh ekstrak Streptomyces sp strain i18, hasil uji fitokimia menunjukkan ekstrak
Streptomyces sp strain i18 memiliki kandungan metabolit sekunder berupa saponin,
triterpenoid dan anthrakuinon glikosida.
Kesimpulan: Ekstrak Streptomyces sp strain i18 tidak memiliki potensi antibiotik terhadap
bakteri Escherichia coli.
Kata Kunci: Streptomyces sp strain i18, Antibiotik, Escherichia coli}
}