%A 1118011064 Karimah Ihda Husnayain
%T PENGARUH EKSTRAK ETANOL DAUN SIRSAK (Annona muricata L.)
TERHADAP KADAR GLUTATION JARINGAN HEPAR TIKUS
(Rattus norvegicus L.) YANG DIINDUKSI 7,12
DIMETHYLBENZ(A)ANTHRACENE (DMBA)
%X Abstrak Bahasa Indonesia

Kanker merupakan suatu proses proliferasi sel di dalam tubuh yang tidak
terkendali. Angka kematian akibat kanker tahun 2012 sebanyak 8,2 juta jiwa dan
hampir 70% dari angka kematian tersebut terdapat di negara berkembang.
Kecenderungan penggunaan antioksidan sebagai zat antikanker herbal semakin
meningkat. Daun sirsak diketahui memiliki sifat sebagai antitumor dan
antioksidan yang dapat memengaruhi kadar antioksidan endogen. Senyawa
DMBA dapat menimbulkan mutasi sel yang berakibat rusaknya parenkim hepar.
Kerusakan ini dapat ditandai penurunan kadar antioksidan endogen seperti
glutation. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian ekstrak
etanol daun sirsak terhadap tumorigenesis hepar tikus yang diinduksi DMBA.
Penelitian terhadap 4 kelompok dengan perlakuan berbeda selama 4 minggu.
Kelompok N (kontrol negatif), P (kontrol positif diinduksi DMBA 20mg/kgBB
2x/minggu, P20 (induksi DMBA 2x/minggu dan ekstrak etanol daun sirsak
20mg/KgBB/hari), P40 (induksi DMBA 2x/minggu dan ekstrak etanol daun sirsak
20mg/KgBB/hari). Hepar diambil dan ditimbang untuk dilakukan pembuatan
homogenat dan pengukuran kadar glutation menggunakan metode Ellman. Hasil
penelitian menunjukkan bahwa kadar glutation hepar rata-rata kelompok N
0,222?0,026?mol/g, P mengalami penurunan (0,208?0,016?mol/g), P20
mengalami peningkatan (0,280?0,012?mol/g), dan P40 meningkat lebih tinggi
(0,325?0,024?mol/g). Hasil penelitan menunjukkan perbedaan signifikan
antarkelompok secara statistik. Hasil uji menunjukkan bahwa pemberian ekstrak
etanol daun sirsak dapat meningkatkan kadar glutation hepar secara signifikan.

Kata kunci: DMBA, ekstrak etanol daun sirsak, glutation, hepar tikus.




Abstract Bahasa Inggris

Cancer is an uncontrolled cell proliferation process in the body. From accounting
in 2012, the mortality rate from cancer shows as much as 8.2 million people and
almost 70% of them are from developing country. The tendency of antioxidants
used as anticancer agents which derived from natural are increase. The content of
the soursop leaves are known as antitumor and antioxidant properties that can
affect the level of endogenous antioxidants. DMBA can cause cell mutations that
could lead into liver parenchyma damage. This damage can be characterized by
the decreased of endogenous antioxidants level such as glutathione. The research
is about the effect of soursop leaves ethanol extract on rat liver tumorigenesis
induced by DMBA. The research conducted on 4 groups with different treatment
for 4 weeks. Group N (negative control), P (positive control DMBA induced 20
mg/kg 2x/week, P20 (DMBA induction 2x/week and soursop leaf ethanol extract
of 20 mg/kg/day), P40 (DMBA induction 2x/week and extracts soursop leaf
ethanol 20 mg/kg/day). The liver is removed and weighed to be making
homogenates and glutathione measurement with Ellman method. The average of
hepatic glutathione levels of each group: group N is 0.222?0.026 mol/g, group P
decreased (0.208?0.016 mol/g), group P20 increased (0.280?0.012 mol/g) and
group P40 increased higher (0.325?0.024 mol/g). Statistically showed significant
differences among the groups. The results is the ethanol extract of soursop leaves
can improve hepatic glutathione levels significantly.

Key word: DMBA, ethanol extract of soursop leaf, glutathione, rat liver
%C Universitas Lampung
%D 2014
%I Fakultas Kedokteran
%L eprints6373