<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n"^^ . "Anggrek merupakan salah satu jenis tanaman hias yang memiliki nilai estetika\r\ntinggi. Salah satu upaya dalam peningkatan performa hortikultura anggrek\r\nDendrobium yaitu melalui hibridisasi tetua-tetua anggrek yang memiliki sifat\r\nunggul dan dilanjutkan dengan perbanyakan biji-biji anggrek hasil silangan\r\nmelalui kultur in vitro. Penelitian ini terdiri dari 2 percobaan. Percobaan I\r\nbertujuan untuk mempelajari pengaruh media dasar dan berbagai konsentrasi air\r\nkelapa ke dalam media kultur serta interaksi keduanya terhadap pengecambahan\r\nin vitro anggrek hasil hibridisasi (D. Wira Pride x D. sutiknoi) x D. Imelda Marina\r\nMasagung. Biji anggrek dikecambahkan pada 6 media perlakuan selama 8\r\nminggu. Percobaan dilaksanakan dalam rancangan acak lengkap (RAL) dengan 4\r\nulangan. Perlakuan disusun secara faktorial 2x3, faktor pertama yaitu 2 jenis\r\nmedia dasar (MS dan pupuk lengkap NPK 32:10:10), sedangkan faktor kedua\r\nadalah konsentrasi air kelapa (0, 75 dan 150 ml/l). Setiap unit percobaan terdiri \r\ndari 1 botol kultur yang ditanami biji anggrek dalam jumlah yang diusahakan\r\nsama (±2,8 mg). Percobaan II bertujuan untuk mempelajari pengaruh jenis adenda\r\norganik dan penambahan KNO3 serta interaksinya terhadap pertumbuhan seedling\r\nanggrek hasil hibridisasi antara (D. Wira Pride x D. sutiknoi) x D. Imelda Marina\r\nMasagung in vitro. Percobaan ini dilaksanakan dalam rancangan acak lengkap\r\n(RAL) dengan 3 ulangan. Perlakuan disusun secara faktorial (3x2), faktor\r\npertama adalah tiga jenis adenda (kentang, tomat, dan pisang), sedangkan faktor\r\nkedua adalah KNO3 (0 dan 2 g/l). Setiap unit percobaan terdiri dari 3 botol kultur\r\nyang berisi masing-masing 10 eksplan. Sebanyak 120 Seedling yang sudah\r\nmemiliki tinggi ≥ 5 cm dengan minimal 4 helai daun dan 4 helai akar\r\ndiaklimatisasi dengan sistem kompot (community pot) menggunakan media\r\nserabut sabut kelapa atau moss putih, 10 seedling per pot. \r\nHasil percobaan I menunjukkan bahwa semua kultur dengan media dasar pupuk\r\nlengkap NPK 32:10:10 menghasilkan perkecambahan biji dan pertumbuhan\r\nprotokorm anggrek (D. Wira Pride x D. sutiknoi) x D. Imelda Marina Masagung,\r\nbaik dengan maupun tanpa penambahan air kelapa, sedangkan kultur biji di media\r\nMS semuanya tidak berkecambah (menghasilkan protokorm). Pemberian air\r\nkelapa sebanyak 150 ml/l menghasilkan skoring jumlah protokorm dan bobot 100\r\nprotokorm tertinggi, diikuti perlakuan air kelapa 75 ml/l dan tanpa penambahan\r\nair kelapa. Hasil percobaan II menunjukkan bahwa adenda kentang secara umum\r\nmenghasilkan pertumbuhan seedling anggrek terbaik diikuti oleh adenda tomat\r\ndan adenda pisang. Pemberian KNO3 2 g/l ke dalam media justru menekan\r\npertumbuhan seedling, hal ini dapat dilihat hampir pada semua variabel\r\npengamatan yaitu tinggi tanaman, jumlah akar, panjang akar, dan bobot segar\r\ntanaman. Semua seedling yang diaklimatisasi, baik dengan media serabut sabut\r\nkelapa maupun moss putih teraklimatisasi 100% setelah berumur 8 minggu sejak\r\ndikeluarkan dari botol. \r\n\r\n\r\nKata kunci : adenda, air kelapa, aklimatisasi, Dendrobium, KNO3, kultur biji,\r\nmoss, MS, pupuk lengkap NPK (32:10:10), sabut kelapa, seedling. \r\n\r\n\r\n \r\nDendrobium, a member of Orchidaceae is a popular ornamental plants with high\r\naesthetical value. Efforts to improve horticultural performance of Dendrobium\r\norchids are always become the breeder’s priority programmes. Hybridization\r\nbetween parent plants with novel characters followed by asymbiotic or in vitro\r\nseed germination is the most easiest way to get a new qualified hybrid. This study\r\nconsisted of two consecutive experiments, i.e., (1) effects of basal media and\r\nvarious concentrations of coconut water on in vitro germination and protocorm\r\ngrowth of hybrid (D. Wira Pride x D. sutiknoi) x D. Imelda Marina Masagung;\r\nand (2) effects of organic addenda and the addition of KNO3 on in vitro growth of\r\nthe hybrid seedlings. The first experiment was conducted in a completely\r\nrandomazed design, replicated four times, with 6 treatments arranged in a (2x3)\r\nfactorial. The first factor was two basal media formulation (MS and NPK\r\n32:10:10 complete fertilizer), while the second factor was the concentrations of \r\ncoconut water (CW) (0. 75 and 150 ml/l). Each experimental unit consisted of 1\r\nculture bottle. Seeds of approximately the same amount (±2.8 mg) from green\r\nmature pod of the hybrid Dendrobium were sowed on six media treatments and\r\nincubated under continuous fluoresence light of approximately 1000 lux, 26±2\r\nC\r\nfor 8 weeks. The second experiment was also carried out in a completely\r\nrandomized design, replicated thrice, with 6 treatments in a (3x2) factorial\r\narrangement. The first factor was three types of medium addenda (potatoe\r\nextract, tomatoe juice, and banana homogenate), while the second factor was\r\naddition of KNO3 (0 and 2 g/l). Each experimental unit consisted of 3 culture\r\nbottles, each of which containing 10 explants. After 12 weeks of cultures, seedling\r\nheight, width of leaves, number of roots, length of roots and seedling freash\r\nweight were recorded. Results of the first experiment showed that all cultures with \r\n0\r\nmedia consisted of complete fertilizer NPK 32:10:10 showed better seed\r\ngermination and protocorm growth, compared to MS medium, in which all the\r\nseeds did not germinate and produce protocorm. Addition of CW at 75 and 150\r\nml/l significantly increased seed germination and protocorm growth, with the\r\nhighest protocorm growth obtained at 150 ml/l CW. This results were showed by\r\nthe highest score of the protocorm mass and weight of 100 protocorms at 150 ml/l\r\nCW, followed by 75 ml/l CW. The results of the second experiment showed that\r\naddition of potato extract generally produced the best orchid seedling growth,\r\nfollowed by tomato and banana. Addition of KNO3 at 2 g/l into the media\r\nsuppressed seedling growth, which was seen in almost all observation variables:\r\nplant height, number of roots, root length, and seedling fresh weight. Following\r\nthose two experiments, the hybrid Dendrobium seedlings were subcultured onto\r\nmedium consisted of NPK 32:10:10 complete fertilizer with 20 g/l sucrose, 100\r\nml/l CW, MS vitamins, 1 g/l tryptone, extract of 200 g/l potatoe and 1 g/l of\r\nactivated charcoal for three months, and incubated under the same culture room\r\nabove. At this stage, hundreds of seedlings with ≥5 cm height, 4-6 leaves and 4-6\r\nroots were obtained. Among 120 seedlings acclimatized with coco-fiber or\r\nshagnum moss media in community pots ex-vitro, 100% of those seedlings were\r\nsuccessfully grew well until 8 weeks.\r\n\r\n\r\nKey words : acclimatization, addenda, coconut fiber, coconut water, Dendrobium,\r\nKNO3, moss, MS, NPK complete fertilizer (32:10:10), seed culture,\r\nseedling. "^^ . "2022-07-25" . . . . . "UNIVERSITAS LAMPUNG"^^ . . . "FAKULTAS PERTANIAN, UNIVERSITAS LAMPUNG"^^ . . . . . . . . . "2024011017 "^^ . " Emi Yunida"^^ . "2024011017 Emi Yunida"^^ . . . . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (File PDF)"^^ . . . "ABSTRAK.pdf"^^ . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (File PDF)"^^ . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (File PDF)"^^ . . . "TESIS TANPA PEMBAHASAN.pdf"^^ . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "indexcodes.txt"^^ . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "preview.jpg"^^ . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "medium.jpg"^^ . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "small.jpg"^^ . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "preview.jpg"^^ . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "medium.jpg"^^ . . . "UPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK\r\nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \r\nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \r\nPEMBESARAN SEEDLING IN VITRO \r\nDAN AKLIMATISASI \r\n (Other)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #64734 \n\nUPAYA PENINGKATAN PERFORMA HORTIKULTURA ANGGREK \nDendrobium MELALUI HIBRIDISASI ANTARA (D. Wira Pride x \nD. sutiknoi) x D. Imelda Marina Masagung: STUDI KULTUR BIJI, \nPEMBESARAN SEEDLING IN VITRO \nDAN AKLIMATISASI \n\n\n" . "text/html" . . . "630 Pertanian dan teknologi yang berkaitan" . .