?url_ver=Z39.88-2004&rft_id=1617011024&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=ISOLASI+DAN+IDENTIFIKASI+BAKTERI+PENGHASIL+LIPASE+TOLERAN+ALKOHOL+DARI+LINGKUNGAN+TANAH+BERMINYAK+SEBAGAI+KATALIS+REAKSI+TRANSESTERIFIKASI+UNTUK+PEMBUATAN+BIODIESEL&rft.creator=SRI+ASTUTI%2C+1617011024&rft.subject=000+Ilmu+komputer%2C+informasi+dan+pekerjaan+umum&rft.description=Produksi+biodiesel+melalui+reaksi+transesterifikasi+secara+enzimatik+telah+banyak+dikembangkan.+Oleh+karena+itu%2Cenzim+yang+digunakan+sebagai+katalis+harus+memiliki+stabilitas+yang+baik+dalam+alkohol.+Pada+penelitian+ini+dilakukan+isolasi+dan+identifikasi+bakteri+penghasil+lipase+toleran+alkohol+dari+sampel+tanah+di+lingkungan+penjual+nasi+goreng.+Isolat+bakteri+yang+diperoleh+diidentifikasi+dan+diuji+aktivitas+lipolitiknya+dalam+pelarut+alkohol+(metanol+dan+etanol)+menggunakan+metode+plate+assay.+Produksi+enzim+juga+dilakukan+untuk+memperoleh+enzim+lipase+yang+selanjutnya+digunakan+sebagai+katalis+reaksi+transesterifikasi+antara+minyak+kelapa+dan+metanol+dengan+perbandingan+1%3A6+sebanyak+10%25+dari+volume+minyak.+Identifikasi+isolat+bakteri+menunjukkan+uji+positif+bakteri+Pseudomonas+sp..+Pseudomonas+sp.+lebih+stabil+dalam+metanol+dibandingkan+dengan+etanol+dengan+kondisi+optimum+pertumbuhan+pada+media+pH+7+dan+waktu+inkubasi+ke-36+jam+dengan+nilai+aktivitas+unit+sebesar+23%2C06+U%2FmL.+Enzim+lipase+yang+telah+dimurnikan+mempunyai+nilai+aktivitas+spesifik+sebesar+22%2C48+U%2Fmg+dan+mempunyai+aktivitas+optimum+pada+suhu+35%E2%84%83%2C+pH+6%2C+dan+konsentrasi+metanol+75%25+dengan+aktivitas+spesifik+sebesar+22%2C76+U%2Fmg.+Hasil+analisis+produk+reaksi+transesterifikasi+menggunakan+GC-MS+menunjukkan+adanya+senyawa+metil+ester+yang+terbentuk+yaitu+metil+laurat+4%2C6%25%3B+metil+miristat+1%2C53%25%3B+dan+metil+palmitat+1%2C11%25.+Jumlah+metil+ester+yang+dihasilkan+menunjukkan+bahwa+reaksi+transesterifikasi+belum+berjalan+dengan+baik+karena+hanya+sedikit+asam+lemak+penyusun+minyak+yang+terkonversi+menjadi+metil+ester.%0D%0A%0D%0AKata+kunci+%3A+lipase%2C+Pseudomonas+sp.%2C+alkohol%2C+transesterifikasi%2C+biodiesel%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0AABSTRACT%0D%0A%0D%0ABiodiesel+production+through+enzymatic+transesterification+reactions+has+been+widely+developed.+Therefore%2C+the+enzyme+used+as+a+catalyst+must+have+good+stability+in+alcohol.+In+this+study%2C+the+isolation+and+identification+of+alcohol-tolerant+lipase-producing+bacteria+from+soil+samples+in+the+fried+rice+seller's+environment+was+carried+out.+The+bacterial+isolates+obtained+were+identified+and+tested+for+their+lipolytic+activity+in+alcohol+solvents+(methanol+and+ethanol)+using+the+plate+assay+method.+Enzyme+production+is+also+carried+out+to+obtain+lipase+enzymes+which+are+then+used+as+a+catalyst+for+transesterification+reactions+between+coconut+oil+and+methanol+with+a+ratio+of+1%3A6+as+much+as+10%25+of+the+volume+of+oil.+Identification+of+bacterial+isolates+showed+a+positive+test+for+Pseudomonas+sp..+Pseudomonas+sp.+more+stable+in+methanol+than+ethanol+with+optimum+growth+conditions+at+pH+7+media+and+incubation+time+of+36+hours+with+unit+activity+value+of+23%2C06+U%2FmL.+The+purified+lipase+enzyme+has+a+specific+activity+value+of+22%2C48+U%2Fmg+and+has+optimum+activity+at+a+temperature+of+35%E2%84%83%2C+pH+6%2C+and+75%25+methanol+concentration+with+a+specific+activity+of+22%2C76+U%2Fmg.+The+results+of+the+analysis+of+the+product+of+the+transesterification+reaction+using+GC-MS+showed+the+presence+of+methyl+ester+compounds+formed%2C+namely+methyl+laurate+4%2C6%25%3B+methyl+myristate+1%2C53%25%3B+and+methyl+palmitate+1%2C11%25.+The+amount+of+methyl+ester+produced+indicates+that+the+transesterification+reaction+has+not+gone+well+because+only+a+few+fatty+acids+that+make+up+the+oil+are+converted+into+methyl+esters.%0D%0A%0D%0AKeywords+%3A+lipase%2C+Pseudomonas+sp.%2C+alcohol%2C+transestesrification%2C+biodiesel&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2022-07-12&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F65456%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F65456%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F65456%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++SRI+ASTUTI%2C+1617011024++(2022)+ISOLASI+DAN+IDENTIFIKASI+BAKTERI+PENGHASIL+LIPASE+TOLERAN+ALKOHOL+DARI+LINGKUNGAN+TANAH+BERMINYAK+SEBAGAI+KATALIS+REAKSI+TRANSESTERIFIKASI+UNTUK+PEMBUATAN+BIODIESEL.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F65456%2F