?url_ver=Z39.88-2004&rft_id=1717011074&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=WAKTU+INKUBASI+OPTIMUM+ACTINOMYCETES+ISOLAT+19B19-A1%0D%0A(Streptomyces+tritolerans)+PADA+MEDIA+SERBUK+KULIT+UDANG&rft.creator=SARAS+KHAIRANI+RACHMAWATI%2C+1717011074&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.description=Enzim+kitinase+merupakan+suatu+enzim+yang+dapat+mendegradasi+kitin+dengan%0D%0Acara+memutus+ikatan+%CE%B2-1%2C4-glikosidik+pada+kitin+untuk+menghasilkan+monomer%0D%0Adan+oligomer+N-Asetilglukosamin.+Actinomycetes+menjadi+salah+satu%0D%0Amikroorganisme+yang+memiliki+kemampuan+kitinolitik.+Penelitian+ini+bertujuan%0D%0Auntuk+memperoleh+waktu+inkubasi+optimum+Actinomycetes+dalam+mendegradasi%0D%0Akulit+udang+menjadi+glukosamin.+Isolat+Actinomycetes+yang+digunakan+diperoleh%0D%0Adari+deposit+UPT+LTSIT%2C+Universitas+Lampung%2C+yang+diisolasi+dari+biota+laut%0D%0Aperairan+Gorontalo%2C+Indonesia.+Penapisan+terhadap+6+isolat+Actinomycetes%2C%0D%0Adiperoleh+isolat+19B19-A1+yang+memiliki+nilai+indeks+kitinolitik+terbesar+dengan%0D%0Anilai+1%2C328.+Kemudian+dilakukan+identifikasi+morfologi+isolat+secara+makroskopis%2C%0D%0Amikroskopis+dan+analisis+filogenetik.+Berdasarkan+hasil+filogenetik+dapat%0D%0Adiindikasikan+bahwa+Isolat+19B19-A1+merupakan+spesies+Streptomyces+tritolerans%0D%0Adengan+kemiripan+sebesar+99%2C41%25.+16S+rRNA+sekuen+gen+isolat+19B19-A1+telah%0D%0Aterdaftar+di+Genbank+dengan+nomor+akses+LC682288.+Pada+penelitian+ini%0D%0Adilakukan+fermentasi+padat+dengan+menggunakan+serbuk+kulit+udang+sebagai%0D%0Amedia.+Fermentasi+dilakukan+selama+14+hari+dengan+pengamatan+setiap+2+hari.%0D%0AAktivitas+kitinase+diuji+menggunakan+spektofotometer+UV-vis+dengan+metode%0D%0ADNS%2C+diperoleh+aktivitas+kitinase+terbesar+pada+waktu+inkubasi+hari+ke-12+dengan%0D%0Anilai+sebesar+0%2C053+U%2FmL.+Produk+degradasi+kulit+udang+dianalisis+menggunakan%0D%0Aspektofotometer+UV-vis+dan+HPLC.+Berdasarkan+hasil+analisis+menggunakan%0D%0Aspektofotometer+UV-vis+diperoleh+glukosamin+tertinggi+pada+waktu+inkubasi+hari%0D%0Ake-12+dengan+konsentrasi+sebesar+0%2C619+mg%2FmL%2C+sedangkan+berdasarkan+hasil%0D%0Aanalisis+menggunakan+HPLC+diperoleh+glukosamin+tertinggi+pada+waktu+inkubasi%0D%0Ahari+ke-14+dengan+konsentrasi+sebesar+1%2C458+mg%2FmL.%0D%0AKata+kunci%3A+Kulit+udang%2C+Kitinase%2C+Actinomycetes%2C+Glukosamin%0D%0A%0D%0AChitinase+enzyme+is+an+enzyme+that+can+degrade+chitin+by+breaking+%CE%B2-1%2C4-%0D%0Aglycosidic+bonds+in+chitin+to+produce+N-Acetylglucosamine+monomers+and%0D%0Aoligomers.+Actinomycetes+is+one+of+the+microorganisms+that+have+abilities+of%0D%0Achitinolytic.+This+study+aimed+to+obtain+the+optimum+incubation+time+of%0D%0AActinomycetes+in+degrading+shrimp+shells+into+glucosamine.+The+Actinomycetes%0D%0Aisolate+used+was+obtained+from+the+UPT+LTSIT+deposit%2C+University+of+Lampung%2C%0D%0Awhich+was+isolated+from+marine+biota+in+the+waters+of+Gorontalo%2C+Indonesia.%0D%0AScreening+of+6+isolates+Actinomycetes%2C+isolate+19B19-A1+was+the+isolate+that+had%0D%0Athe+largest+chitinolytic+index+value+with+a+value+of+1.328.+Identified+the%0D%0Amorphology+of+the+isolates+by+macroscopic%2C+microscopic+and+phylogenetic%0D%0Aanalysis.+Based+on+the+phylogenetic+results%2C+it+can+be+indicated+that+isolate+19B19-%0D%0AA1+is+a+species+of+Streptomyces+tritolerans+with+a+similarity+99.41%25.+16S+rRNA%0D%0Agene+sequence+isolate+19B19-A1+was+registered+at+Genbank+with+access+number%0D%0ALC682288.+In+this+study%2C+solid+fermentation+was+carried+out+using+shrimp+shell%0D%0Apowder+as+a+medium.+Fermentation+was+carried+out+for+14+days+with+observations%0D%0Aevery+2+days.+Chitinase+activity+was+tested+using+a+UV-vis+spectrophotometer+with%0D%0Athe+DNS+method%2C+the+highest+chitinase+activity+was+obtained+on+the+12th+day+of%0D%0Aincubation+with+a+value+of+0.053+U%2FmL.+Shrimp+shell+degradation+products+were%0D%0Aanalyzed+using+spectrophotometer+UV-vis+and+HPLC.+Based+on+the+analysis+using%0D%0Aspectrophotometer+UV-vis%2C+the+highest+glucosamine+was+obtained+on+the+12th+day%0D%0Aof+incubation+with+a+concentration+of+0.619+mg%2FmL%2C+while+based+on+the+results+of%0D%0Athe+analysis+using+HPLC%2C+the+highest+glucosamine+was+obtained+on+the+14th+day%0D%0Aof+incubation+with+a+concentration+of+1.458+mg%2FmL.%0D%0AKey+words%3A+Shrimp+shell%2C+Chitinase%2C+Actinomycetes%2C+Glucosamine.%0D%0A%0D%0A&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2022-08-06&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F65737%2F2%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F65737%2F1%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F65737%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++SARAS+KHAIRANI+RACHMAWATI%2C+1717011074++(2022)+WAKTU+INKUBASI+OPTIMUM+ACTINOMYCETES+ISOLAT+19B19-A1+(Streptomyces+tritolerans)+PADA+MEDIA+SERBUK+KULIT+UDANG.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F65737%2F