creators_name: LISA MARYATI, 1757021002
creators_id: lisamaryati05@gmail.com
type: other
datestamp: 2022-08-26 07:52:27
lastmod: 2022-08-26 07:52:27
metadata_visibility: show
title: UJI AKTIVITAS SENYAWA ANTIBAKTERI Bacillus sp. DARI TANAH
KEBUN RAYA LIWA LAMPUNG BARAT TERHADAP Dickeya sp.  



ispublished: pub
subjects: 500
subjects: 570
full_text_status: restricted
abstract:  
Kelompok Bacillus tanah banyak dilaporkan menghasilkan senyawa
antibakteri terhadap bakteri patogen tanaman. Salah satu bakteri patogen tanaman
yaitu Dickeya sp. yang menyebabkan penyakit busuk lunak pada organ daun,
batang, dan akar tanaman. Kebun Raya Liwa (KRL) Lampung Barat memiliki
kondisi tanah dan lingkungan yang memungkinkan ditemukannya keberadaan
genus Bacillus. Telah berhasil ditemukan isolat Bacillus sp. TSR 6 asal tanah
KRL. Isolat Bacillus sp.  kode TSR 6 merupakan hasil skrining terbaik pada uji
antagonis terhadap Dickeya sp. Penelitian ini bertujuan mengetahui aktivitas
senyawa antibakteri Bacillus sp. asal tanah KRL terhadap Dickeya sp. Isolat
Bacillus sp. TSR 6 dikulturkan pada medium produksi antibakteri cair dan
dilakukan ekstraksi menghasilkan ekstrak kasar etil asetat (222 mg) dan
menunjukkan aktivitas antibakteri terhadap Dickeya sp. Pemisahan senyawa
ekstrak kasar dengan kromatografi kolom terbuka menghasilkan 8 fraksi dan
terdapat 3 fraksi aktif yang menunjukkan hambatan terhadap Dickeya sp. pada uji
aktivitas antibakteri melalui difusi agar cakram. Fraksi 54 memperlihatkan daya
hambat terbesar dan tergolong kuat dengan zona hambat 13,5 mm pada
konsentrasi stok 5000 μg/ml. Fraksi tersebut memiliki Kadar Hambat Minimum
(KHM) pada konsentrasi 500 μg/ml berdasarkan nilai turbiditas dan pewarnaan
resazurin melalui mikrodilusi cair. Hasil identifikasi Kromatografi Lapis Tipis
(KLT) pada fraksi aktif dengan eluen n-heksana:etil asetat (7:3) menunjukkan
senyawa polar yang positif membentuk noda keunguan terhadap ninhidrin. Hal
tersebut mengindikasikan bahwa senyawa pada fraksi aktif mengandung senyawa
peptida.  


Kata kunci: antibakteri, Bacillus sp., Kadar Hambat Minimum, Kromatografi 
Lapis Tipis.
  


 
Soil Bacillus groups have been reported to produce antibacterial compounds
against plant pathogenic bacteria. One of the plant pathogenic bacteria is Dickeva
sp. which causes soft rot disease in leaf, stems, and roots of plants organ. Liwa
Botanical Garden (KRL) West Lampung has soil and environmental conditions
that allow existence of the genus Bacillus. It has been successfully found isolates
of Bacillus sp. TSR 6 from the soil of the KRL. The isolate with code TSR 6 of
Bacillus sp. showed the best antagonistic activity against Dickeya sp. This study
aims to determine antibacterial compound activity of the Bacillus sp. from soil in
KRL against Dickeya sp. Bacillus sp. TSR 6 was cultured on liquid antibacterial
production medium and extracted to produce crude ethyl acetate extract (222 mg)
and showed antibacterial activity against Dickeya sp. Fractionation of compounds
in crude extract by open column chromatography resulted in 8 fractions and there
were 3 active fractions which showed inhibition against Dickeya sp. on the
antibacterial activity test via agar disc diffusion. Fraction 54 showed the strongest
inhibition with an inhibition zone 13.5 mm at a stock concentration 5000 ug/ml.
This fraction has a Minimum Inhibitory Concentration (MIC) at concentration 500
g/ml based on turbidity value and resazurin assay via broth microdilution. The
identification of compounds in active fractions by Thin Layer Chromatography
(TLC) with eluent n-Hexane:IPA (7:3) showed polar compounds and positive
results in ninhydrin by purplish stain. This indicated the compounds in active
fractions have peptide compounds.


Keywords: Antibacterial, Bacillus sp., Minimum Inhibitory Concentration, Thin 
Layer Chromatography.
  
date: 2022-07-20
date_type: published
publisher: FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM 
place_of_pub: UNIVERSITAS LAMPUNG
citation:   LISA MARYATI, 1757021002  (2022) UJI AKTIVITAS SENYAWA ANTIBAKTERI Bacillus sp. DARI TANAH KEBUN RAYA LIWA LAMPUNG BARAT TERHADAP Dickeya sp.  FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM , UNIVERSITAS LAMPUNG.     
document_url: http://digilib.unila.ac.id/65750/1/ABSTRAK.pdf
document_url: http://digilib.unila.ac.id/65750/2/SKRIPSI%20FULL.pdf
document_url: http://digilib.unila.ac.id/65750/3/SKRIPSI%20TANPA%20BAB%20PEMBAHASAN.pdf