?url_ver=Z39.88-2004&rft_id=1717021018&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=KEANEKARAGAMAN+Bacillus+DARI+TANAH+KEBUN+RAYA+LIWA&rft.creator=VIDIA+ROYANTI%2C+1717021018&rft.subject=000+Ilmu+komputer%2C+informasi+dan+pekerjaan+umum&rft.description=Kebun+Raya+Liwa+salah+satu+tempat+pengelolaan+tanaman+hias+yang+terletak+di+Liwa+kabupaten+Lampung+Barat%2C+memiliki+luas+lahan+86+ha.+Kawasan+Kebun+Raya+Liwa+terletak+pada+ketinggian+antara+890-948+mdpl+dimana+pada+ketinggian+ini+termasuk+kedalam+daerah+perbukitan.+Kawasan+Kebun+Raya+Liwa+memiliki+kondisi+topografi+yang+bervariasi+sehingga+memiliki+kondisi+tanah+yang+berbeda-beda%2C+hal+ini+dapat+memicu+adanya+keragaman+mikroorganisme+tanah+salah+satunya+Bacillus.+Bacillus+dapat+dijumpai+di+daerah+rhizofer.+Terdapat+beberapa+jenis+Bacillus+diantaranya+adalah+Bacillus+sp.+Bacillus+subtilis%2C+Bacillus+cereus%2C+Bacillus+coagulans%2C+Bacillus+lichenoformis.+Bakteri+Bacillus+dapat+bertahan+hidup+pada+suhu+45-55oC+dengan+suhu+minimum+5-20oC.+Tujuan+dari+penelitian+ini+untuk+memperoleh+isolat+Bacillus+dari+tanah+Kebun+Raya+Liwa+serta+dapat+mengetahui+keragaman+yang+ada+di+Kebun+Raya+Liwa.+Isolat+Bacillus+dapat+diperoleh+dengan+dikarakterisasi+morfologi+koloni%2C+morfologi+sel+(pengecatan+gram+dan+pengecatan+spora)%2C+uji+fisiologi+(uji+katalase+dan+uji+motilitas)+serta+dilakukan+uji+enzimatik+(selulase%2C+protease%2C+kitinase%2C+dan+lipase).+Data+diperoleh+dengan+dianalisis+secara+deskriptif.%0D%0AHasil+penelitian+ini+diperoleh+keanekaragaman+bakteri+pada+sampel+tanah+Kebun+Raya+Liwa+dengan+mengacu+pada+perhitungan+Shanon+Wiener+didapatkan+hasil+keragaman+bakteri+tertinggi+diperoleh+pada+tanah+serasah+dengan+total+nilai+2%2C48+yang+menunjukan+keanekaragaman+tingkat+sedang+sedangkan+untuk+total+nilai+terendah+diperoleh+pada+tanah+biopori+dengan+nilai+1%2C35+yang+menunjukan+keanekaragaman+bakteri+tingkat+sedang.+Tingkat+tinggi+dan+rendahnya+suatu+keanekaragaman+bakteri+Bacillus+dapat+diakibatkan+karena+kondisi+lingkungan+yang+memenuhi+kebutuhan+nutrisi+dari+bakteri+Bacilllus.+Dalam+penelitian+ini+didapatkan+bentuk+koloni+yang+banyak+ditemukan+yaitu+bentuk+koloni+circular.+Untuk+hasil+uji+karakterisasi+yang+dilakukan+dengan+beberapa+uji+seperti+hasil+uji+pengecatan+gram+yang+menghasilkan+Gram+positif%2C+untuk+pengecatan+spora+diperoleh+hasil+positif+yaitu+bakteri+memiliki+spora%2C+sedangkan+untuk+uji+enzimatik+ditemukan+adanya+aktivitas+enzim+yang+ditandai+dengan+adanya+zona+jernih.%0D%0A%0D%0AKata+kunci+%3A+keanekaragaman%2C+Bacillus%2C+tanah%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0Aabstarct%0D%0A%0D%0ALiwa+Botanical+Gardens%2C+one+of+the+ornamental+plant+management+sites%2C+located+in+Liwa%2C+West+Lampung+district%2C+has+a+land+area+of+86+ha.+The+Liwa+Botanical+Gardens+area+is+located+at+an+altitude+between+890-948+meters+above+sea+level+where+at+this+altitude+is+included+in+a+hilly+area.+The+Liwa+Botanical+Gardens+area+has+varied+topographic+conditions+so+that+it+has+different+soil+conditions%2C+this+can+trigger+a+diversity+of+soil+microorganisms%2C+one+of+which+is+Bacillus.+Bacillus+can+be+found+in+the+rhizofer+region.+There+are+several+types+of+Bacillus+including+Bacillus+sp.+Bacillus+subtilis%2C+Bacillus+cereus%2C+Bacillus+coagulans%2C+Bacillus+lichenoformis.+Bacillus+bacteria+can+survive+at+a+temperature+of+45-55oC+with+a+minimum+temperature+of+5-20oC.+The+purpose+of+this+study+was+to+obtain+Bacillus+isolates+from+the+soil+of+the+Liwa+Botanical+Gardens+and+to+determine+the+diversity+in+the+Liwa+Botanical+Gardens.+Bacillus+isolates+could+be+obtained+by+characterizing+colony+morphology%2C+cell+morphology+(gram+staining+and+spore+staining)%2C+physiological+tests+(catalase+and+motility+tests)+and+enzymatic+tests+(cellulase%2C+protease%2C+chitinase%2C+and+lipase).+Data+obtained+by+analyzing+descriptively.%0D%0AThe+results+of+this+study+obtained+the+diversity+of+bacteria+in+the+soil+sample+of+the+Liwa+Botanical+Gardens+with+reference+to+Shanon+Wiener's+calculations%2C+the+results+of+the+highest+bacterial+diversity+were+obtained+in+litter+soil+with+a+total+value+of+2.48+which+showed+a+moderate+level+of+diversity+while+the+lowest+total+value+was+obtained+in+biopore+soils+with+a+value+of+1+%2C35+which+indicates+a+moderate+level+of+bacterial+diversity.+High+and+low+levels+of+a+diversity+of+Bacillus+bacteria+can+be+caused+by+environmental+conditions+that+meet+the%0D%0Av%0D%0Anutritional+needs+of+Bacillus+bacteria.+In+this+study%2C+the+most+common+forms+of+colonies+found+were+circular+colonies.+For+the+results+of+the+characterization+test+carried+out+with+several+tests+such+as+the+results+of+the+gram+staining+test+which+resulted+in+Gram+positive%2C+for+the+spore+staining+positive+results+were+obtained%2C+namely+bacteria+had+spores%2C+while+for+the+enzymatic+test+it+was+found+that+there+was+enzyme+activity+which+was+indicated+by+the+presence+of+a+clear+zone.%0D%0A%0D%0AKeywords%3A+diversity%2C+Bacillus%2C+soil&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2022-08-11&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F65799%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F65799%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F65799%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++VIDIA+ROYANTI%2C+1717021018++(2022)+KEANEKARAGAMAN+Bacillus+DARI+TANAH+KEBUN+RAYA+LIWA.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F65799%2F