?url_ver=Z39.88-2004&rft_id=1717011003&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=ISOLASI+DAN+OPTIMASI+PRODUKSI+BACTERIAL+NANOCELLULOSE%0D%0A(BNC)+OLEH+MIKROBA+ISOLAT+LOKAL+Kc-S-1&rft.creator=GISTA+KUSUMA+RINA%2C+1717011003&rft.subject=000+Ilmu+komputer%2C+informasi+dan+pekerjaan+umum&rft.description=Produksi+Bacterial+Nanocellulose+(BNC)+dapat+dioptimalkan+dengan+pemilihan%0D%0Asumber+karbon+dan+sumber+nitrogen+yang+tepat%2C+sehingga+waktu+inkubasi+produksi%0D%0Alebih+cepat+dan+menghemat+biaya+produksi.+Penelitian+ini+bertujuan+untuk%0D%0Amemperoleh+isolat+bakteri+penghasil+BNC+dari+kombucha+dan+memperoleh+berat%0D%0Apelikel+dan+kecepatan+produksi+BNC+yang+optimal+dari+sumber+karbon+dan%0D%0Asumber+nitrogen+dengan+berbagai+variasi+konsentrasi.+Produksi+BNC+diawali%0D%0Adengan+isolasi+bakteri+penghasil+BNC.+Kemudian+dilakukan+produksi+BNC%0D%0Amenggunakan+sumber+karbon+dengan+variasi+konsentrasi+2%25%2C+2%2C5%25%2C+5%25%2C+10%25+dan%0D%0Asumber+nitrogen+dengan+variasi+konsentrasi+1%25%2C+1%2C5%25%2C+2%25%2C+2%2C5%25+pada+keadaan%0D%0Astatis+dan+agitasi+dengan+kecepatan+150+rpm.+Selanjutnya+dilakukan+pengukuran%0D%0Aberat+pelikel+dan+Water+Hold+Capacity+(WHC)+BNC.+Lalu+dilakukan+analisis%0D%0Ahidrolisis+asam+menggunakan+spektrofotometer+UV-Vis.+Hasil+penelitian%0D%0Amenunjukkan+bahwa+isolat+Kc-S-1+mampu+menghasilkan+pelikel+seberat+8%2C7+g%0D%0Adengan+waktu+inkubasi+7+hari+secara+statis.+Sumber+karbon+terbaik+untuk+produksi%0D%0ABNC+adalah+glukosa+5%25+yang+menghasilkan+pelikel+seberat+4%2C0+g.+Sumber%0D%0Anitrogen+terbaik+adalah+beef+extract+yang+menghasilkan+pelikel+seberat+5%2C7+g%0D%0Adengan+waktu+inkubasi+7+hari+secara+statis.+Beef+extract+2%25+menghasilkan+pelikel%0D%0Adengan+berat+terbaik+sebesar+16%2C0+g+pada+waktu+inkubasi+14+hari+secara+statis.%0D%0AProduksi+BNC+lebih+baik+pada+kondisi+statis+dibandingkan+dengan+kondisi+agitasi.%0D%0AWHC+pada+kondisi+terbaik+sebesar+97%2C5%25.+Analisis+BNC+menggunakan+metode%0D%0Ahidrolisis+asam+menghasilkan+larutan+berwarna+kuning+kecoklatan+dan+absorbansi%0D%0Asebesar+0%2C6022.+Dapat+disimpulkan+bahwa+sumber+karbon+jenis+glukosa+dan%0D%0Asumber+nitrogen+jenis+beef+extract+mampu+menghasilkan+BNC+yang+optimal.%0D%0A%0D%0AKata+Kunci%3A+Bacterial+Nanocellulose+(BNC)%2C+sumber+karbon%2C+sumber+nitrogen.%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0AABSTRACT%0D%0A%0D%0AProduction+Bacterial+Nanocellulose+(BNC)+can+be+optimized+by+selecting+the+right%0D%0Acarbon+source+and+nitrogen+source%2C+so+that+the+production+incubation+time+is+faster%0D%0Aand+saves+production+costs.+This+study+aimed+to+obtain+isolates+of+BNC-producing%0D%0Abacteria+from+kombucha+and+obtain+optimal+pellicle+weight+and+BNC+production%0D%0Aspeed+from+carbon+sources+and+nitrogen+sources+with+various+concentrations.%0D%0ABNC+production+begins+with+the+isolation+of+BNC-producing+bacteria.+Then+BNC%0D%0Aproduction+was+carried+out+using+carbon+sources+with+various+concentrations+of%0D%0A2%25%2C+2.5%25%2C+5%25%2C+10%25+and+nitrogen+sources+with+varying+concentrations+of+1%25%2C%0D%0A1.5%25%2C+2%25%2C+2.5%25+in+static+and+agitated+conditions.+speed+150+rpm.+Subsequently%2C%0D%0Athe+pellicle+weight+and+were+measured+Water+Hold+Capacity+(WHC)+BNC.+Then%0D%0Athe+acid+hydrolysis+analysis+was+carried+out+using+UV-Vis+spectrophotometer.+The%0D%0Aresults+showed+that+Kc-S-1+isolate+was+able+to+produce+pellicle+weighing+8.7+g%0D%0Awith+an+incubation+time+of+7+days+statically.+The+best+carbon+source+for+BNC%0D%0Aproduction+is+5%25+glucose+which+produces+a+pellicle+weighing+4.0+g.+The+best%0D%0Anitrogen+source+is+beef+extract+which+produced+a+pellicle+weighing+5.7+g+with+an%0D%0Aincubation+time+of+7+days+statically.+Beef+extract+2%25+produced+pellicle+with+the%0D%0Abest+weight+of+16.0+g+at+14+days+incubation+statically.+BNC+production+was+better%0D%0Aunder+static+conditions+than+agitated+conditions.+WHC+in+the+best+condition+is%0D%0A97.5%25.+BNC+analysis+using+acid+hydrolysis+method+produces+a+brownish+yellow%0D%0Asolution+and+an+absorbance+of+0.6022.+It+can+be+concluded+that+the+carbon+source%0D%0Atype+is+glucose+and+the+nitrogen+source+is+type+beef+extract+able+to+produce%0D%0Aoptimal+BNC.%0D%0A%0D%0AKeywords%3A+Bacterial+Nanocellulose+(BNC)%2C+carbon+source%2C+nitrogen+source.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2022-08-09&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F65897%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F65897%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F65897%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++GISTA+KUSUMA+RINA%2C+1717011003++(2022)+ISOLASI+DAN+OPTIMASI+PRODUKSI+BACTERIAL+NANOCELLULOSE+(BNC)+OLEH+MIKROBA+ISOLAT+LOKAL+Kc-S-1.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F65897%2F