?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=OPTIMASI+WAKTU+INKUBASI+DAN+KADAR+TRYPTOPHAN%0D%0ATERHADAP+PRODUKSI+HORMON+INDOLE+ACETIC+ACID+(IAA)%0D%0AISOLAT+Streptomyces+hygroscopicus+SUBSP.+hygroscopicus+STRAIN+I18&rft.creator=Mia+Fitriani%2C+1717021055&rft.subject=570+Biologi&rft.description=Proses+pertumbuhan+tanaman+memerlukan+hormon%2C+misalnya+hormon+auksin+yang%0D%0Aberguna+untuk+mempercepat+pertumbuhan+suatu+tanaman.+Hormon+auksin+dapat%0D%0Adihasilkan+oleh+tanaman+itu+sendiri+dan+makhluk+hidup+seperti+Actinomycetes.%0D%0AActinomycetes+merupakan+jenis+rizobakteri+pemacu+pertumbuhan+tanaman+yang%0D%0Adapat+mempengaruhi+secara+langsung+dan+tidak+langsung+proses+pertumbuhan%0D%0Atanaman.+Salah+satu+spesies+dari+kelas+Actinomycetes+yang+dapat+menghasilkan%0D%0Asenyawa+fitohormon+IAA+adalah+Streptomyces+hygroscopicus.+Dalam%0D%0Apertumbuhan%2C+IAA+dapat+berperan+dalam+proses+perkembangan+tanaman%2C%0D%0Apemanjangan+akar%2C+pucuk%2C+dan+pematangan+buah.+Penelitian+ini+dilakukan+di%0D%0ALaboratorium+Mikrobiologi+FMIPA+Universitas+Lampung+dengan+2+tahap%0D%0Apenelitian.+Tahap+pertama+mengetahui+waktu+optimum+inkubasi+isolat%0D%0AStreptomyces+hygroscopicus+dalam+menghasilkan+IAA+dan+tahap+kedua%0D%0Amengetahui+pengaruh+penambahan+Tryptophan+dalam+menghasilkan+IAA.%0D%0APengukuran+dilakukan+pada+hari+inkubasi+4-11+dan+penambahan+Tryptophan+yaitu%0D%0A0+mg%2FmL%2C+1+mg%2FmL%2C+2+mg%2FmL%2C+3+mg%2FmL%2C+4+mg%2FmL%2C+5+mg%2FmL%2C+dan+6+mg%2FmL+yang%0D%0Amasing-masing+perlakuan+diulang+sebanyak+3+kali.+Parameter+pada+penelitian+ini%0D%0Aadalah+perubahan+warna+merah+supernatan+yang+direaksikan+dengan+reagen%0D%0ASalkowski%2C+jika+terdapat+perubahan+warna+maka+diukur+menggunakan%0D%0Aspektrofotometer+panjang+gelombang+530+nm.+Rancangan+yang+digunakan+adalah%0D%0ARancangan+Acak+Lengkap+(RAL)+dan+hasil+dianalisis+menggunakan+persamaan%0D%0Aregresi+polinomial+ortogonal.+Kesimpulan+dari+penelitian+ini+diperoleh+waktu%0D%0Aoptimum+inkubasi+Streptomyces+hygroscopicus+subsp.+hygroscopicus+strain+I18%0D%0Aadalah+hari+ke-9+dengan+konsentrasi+IAA+sebesar+14%2C3+ppm+dan+penambahan%0D%0ATryptophan+optimum+adalah+5+mg%2FmL+dengan+konsentrasi+IAA+sebesar+18%2C4+ppm.%0D%0AKata+Kunci+%3A+Hormon+auksin%2C+Actinomycetes%2C+Streptomyces+hygroscopicus%0D%0ATryptophan.%0D%0A%0D%0AThe+process+of+plant+growth+requires+hormones%2C+such+as+the+hormone+auxin+which%0D%0Ais+useful+for+accelerating+the+growth+of+a+plant.+Auxin+hormones+can+be+produced%0D%0Aby+plants+themselves+and+living+things+such+as+Actinomycetes.+Actinomycetes+is+a%0D%0Atype+of+plant+growth-promoting+rhizobacteria+that+can+directly+and+indirectly+affect%0D%0Aplant+growth+processes.+One+of+the+species+from+the+Actinomycetes+class+that+can%0D%0Aproduce+IAA+phytohormones+is+Streptomyces+hygroscopicus.+In+growth%2C+IAA+can%0D%0Aplay+a+role+in+the+process+of+plant+development%2C+elongation+of+roots%2C+shoots%2C+and+fruit%0D%0Aripening.+This+research+was+conducted+at+the+Laboratory+of+Microbiology+FMIPA%0D%0AUniversity+of+Lampung+with+2+stages+of+research.+The+first+stage+is+to+determine+the%0D%0Aoptimum+incubation+time+is+Streptomyces+hygroscopicus+in+producing+IAA+and+the%0D%0Asecond+stage+is+to+determine+the+effect+of+the+addition+of+Tryptophan+in+producing%0D%0AIAA.+Measurements+were+made+on+incubation+days+4-11+and+the+addition+of%0D%0ATryptophan+was+0+mg%2FmL%2C+1+mg%2FmL%2C+2+mg%2FmL%2C+3+mg%2FmL%2C+4+mg%2FmL%2C+5+mg%2FmL+and%0D%0A6+mg%2FmL%2C+each+treatment+was+repeated+3+times.+The+parameter+in+this+study+is+the%0D%0Ared+color+change+of+the+supernatant+which+is+reacted+with+Salkowski's+reagent%2C+if%0D%0Athere+is+a+color+change+then+it+is+measured+using+a+spectrophotometer+with+a%0D%0Awavelength+of+530+nm.+The+design+used+was+Completely+Randomized+Design%0D%0A(CRD)+and+the+results+of+the+analysis+used+orthogonal+polynomial+regression%0D%0Aequations.+The+conclusion+of+this+research+is+that+the+optimum+incubation+time+of%0D%0AStreptomyces+hygroscopicus+subsp.+hygroscopicus+strain+I18+was+the+9th+day+with%0D%0AIAA+concentration+of+14.3+ppm+and+the+optimum+addition+of+Tryptophan+was+5%0D%0Amg%2FmL+with+IAA+concentration+of+18.4+ppm.%0D%0AKeywords%3A+Auxin+hormone%2C+Actinomycetes%2C+Streptomyces+hygroscopicus%0D%0ATryptophan.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2021-08-05&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F66951%2F1%2Fabstrak%2520-%2520Mia%2520Fitriani.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F66951%2F2%2Ffull%2520skripsi%2520tanpa%2520lampiran%2520-%2520Mia%2520Fitriani.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F66951%2F3%2Ffull%2520skripsi%2520tanpa%2520lampiran%2520dan%2520pembahasan%2520-%2520Mia%2520Fitriani.pdf&rft.identifier=++Mia+Fitriani%2C+1717021055++(2021)+OPTIMASI+WAKTU+INKUBASI+DAN+KADAR+TRYPTOPHAN+TERHADAP+PRODUKSI+HORMON+INDOLE+ACETIC+ACID+(IAA)+ISOLAT+Streptomyces+hygroscopicus+SUBSP.+hygroscopicus+STRAIN+I18.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F66951%2F