?url_ver=Z39.88-2004&rft_id=1817011091&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=EKSTRAKSI+DAN+UJI+AKTIVITAS+OPTIMUM+ENZIM+KITINASE+PADA+ACTINOMYCETES+19B19A1+MENGGUNAKAN+MEDIA+LIMBAH+KULIT+UDANG&rft.creator=IRMA+FITRIA+ANANDA%2C+1817011091&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=660+Teknik+kimia&rft.description=Enzim+kitinase+merupakan+suatu+enzim+yang+dapat+mendegradasi+kitin+dengan+cara+memutus+ikatan+%CE%B2-1%2C4-glikosidik+pada+kitin+untuk+menghasilkan+monomer+dan+oligomer+N-Asetilglukosamin.+Actinomycetes+menjadi+salah+satu+mikroorganisme+yang+memiliki+kemampuan+kitinolitik.+Penelitian+ini+bertujuan+untuk+memperoleh+pH+dan+waktu+optimum+enzim+kitinase+pada+Actinomycetes+19B19A1+dalam+menggunakan+media+limbah+kulit+udang.+Isolat+Actinomycetes+yang+digunakan+diperoleh+dari+deposit+UPT+LTSIT%2C+Universitas+Lampung%2C+yang+diisolasi+dari+biota+laut+perairan+Gorontalo%2C+Indonesia.+Isolat+19B19A1+memiliki+nilai+indeks+kitinolitik+sebesar+1%2C26+cm.+Kemudian+dilakukan+identifikasi+morfologi+isolat+secara+mikroskopis.+Berdasarkan+hasil+mikroskop+tersebut+dapat+diindikasikan+bahwa+isolat+19B19A1+merupakan+spesies+Streptomyces.+Pada+penelitian+ini+dilakukan+fermentasi+padat+dengan+menggunakan+serbuk+kulit+udang+sebagai+media.+Fermentasi+dilakukan+selama+14+hari+dengan+pengamatan+setiap+2+hari.+Aktivitas+kitinase+diuji+menggunakan+spektofotometer+UV-Vis+dengan+metode+DNS.+Berdasarkan+hasil+analisis+menggunakan+spektofotometer+UV-Vis+diperoleh+aktivitas+spesifik+enzim+kitinase+tertinggi+pada+waktu+inkubasi+hari+ke-10+sebesar+2.6426+U%2Fmg.+Kemudian+ekstrak+kasar+enzim+kitinase+tersebut+dilakukan+pemurnian+dengam+menggunakan+ammonium+sulfat+dan+dialisis+diperoleh+hasil+aktivitas+spesifik+sebesar+7.5080+U%2Fmg.%0D%0A%0D%0AKata+kunci%3A+Actinomycetes%2C+Enzim+kitinase%2C+Kulit+udang.%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0AABSTRACT%0D%0A%0D%0AChitinase+is+an+enzyme+that+can+degrade+chitin+by+breaking+the+-1%2C4-glycosidic+enzyme+in+chitin+to+produce+N-Acetylglucosamine+monomers+and+oligomers.+Actinomycetes+are+one+of+the+microorganisms+that+have+chitinolytic+abilities.+This+study+aims+to+obtain+the+optimum+pH+and+time+of+chitinase+in+Actinomycetes+19B19A1+using+shrimp+shell+waste+media.+Actinomycetes+isolates+used+were+from+the+UPT+LTSIT+deposit%2C+University+of+Lampung%2C+which+were+isolated+from+marine+biota+in+the+waters+of+Gorontalo%2C+Indonesia.+The+19B19A1+isolate+had+a+chitinolytic+index+value+of+1.26+cm.+Then+the+morphology+of+the+isolates+was+carried+out+microscopically.+Based+on+the+results+of+the+microscope%2C+it+can+be+indicated+that+Isolate+19B19A1+is+a+Streptomyces+species.+In+this+study%2C+solid+fermentation+was+carried+out+using+shrimp+shell+powder+as+a+medium.+Fermentation+was+carried+out+for+14+days+with+observations+every+2+days.+Chitinase+activity+was+tested+using+a+UV-Vis+spectrophotometer+with+the+DNS+method.+the+results+of+the+analysis+using+UV-Vis+spectrophotometer+obtained+the+highest+specific+activity+of+the+chitinase+enzyme+at+the+incubation+time+based+on+the+10th+day+of+2.6426+U%2Fmg.+Then+the+crude+extract+of+the+chitinase+enzyme+was+purified+using+ammonium+sulfate+and+dialysis%2C+the+specific+activity+was+7.5080+U%2Fmg.%0D%0A%0D%0AKey+words%3A+Actinomycetes%2C+Chitinase+enzyme%2C+Shrimp+shell.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2022-11-28&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F67793%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F67793%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F67793%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++IRMA+FITRIA+ANANDA%2C+1817011091++(2022)+EKSTRAKSI+DAN+UJI+AKTIVITAS+OPTIMUM+ENZIM+KITINASE+PADA+ACTINOMYCETES+19B19A1+MENGGUNAKAN+MEDIA+LIMBAH+KULIT+UDANG.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F67793%2F