?url_ver=Z39.88-2004&rft_id=1817011053&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=PENGARUH+MODIFIKASI+KIMIA+MENGGUNAKAN%0D%0AASAM+GLIOKSILAT+TERHADAP+STABILITAS+ENZIM+%CE%B1-AMILASE+DARI+Aspergillus+fumigatus&rft.creator=Dwi+Noviani+%2C+dwinoviani52%40gmail.com&rft.subject=000+Ilmu+komputer%2C+informasi+dan+pekerjaan+umum&rft.subject=001+Ilmu+pengetahuan&rft.description=ABSTRAK%0D%0APENGARUH+MODIFIKASI+KIMIA+MENGGUNAKAN%0D%0AASAM+GLIOKSILAT+TERHADAP+STABILITAS+ENZIM+%CE%B1-AMILASE+DARI+Aspergillus+fumigatus%0D%0AOleh%0D%0ADwi+Noviani%0D%0AEnzim+%CE%B1-amilase+merupakan+enzim+ekstraseluler+yang+mampu+memotong+ikatan+1%2C4-%CE%B1-D-glikosidik+antar+monomer+glukosa+pada+rantai+linier+amilosa.+Enzim+%CE%B1-amilase+memiliki+aplikasi+paling+luas+dalam+berbagai+bidang+industri.+Penelitian+ini+bertujuan+untuk+meningkatkan+stabilitas+enzim+%CE%B1-amilase+dari+A.+fumigatus+dengan+modifikasi+kimia+menggunakan+asam+glioksilat.+Enzim+%CE%B1-amilase+diisolasi+menggunakan+sentrifugasi+dan+dimurnikan+menggunakan+fraksinasi+dengan+amonium+sulfat+dan+dialisis.+Enzim+hasil+pemurnian+dimodifikasi+dengan+asam+glioksilat.+Aktivitas+enzim+%CE%B1-amilase+dilakukan+dengan+metode+Fuwa+dan+metode+Mandels.+Kadar+protein+ditentukan+dengan+metode+Lowry.+Hasil+penelitian+menunjukkan+aktivitas+spesifik+enzim+hasil+pemurnian+sebesar+1.037%2C42+U%2Fmg+meningkat+18+kali+dibandingkan+dengan+ekstrak+kasar+enzim+yang+mempunyai+aktivitas+spesifik+58%2C31+U%2Fmg.+Enzim+hasil+pemurnian+mempunyai+pH+optimum+5%2C0+dengan+suhu+optimum+55%EF%82%B0C%3B+KM+%3D+11%2C55+mg%2FmL-1+substrat%2C+Vmaks+%3D+10%2C76+%CE%BCmol+mL-1menit-1dan+uji+stabilitas+termal+enzim+selama+100+menit+pada+suhu+60oC+memiliki+aktivitas+sisa+sebesar+9%2C34%25+dengan+ki+%3D+0%2C018+menit-1%3B+t1%2F2+%3D+38%2C50+menit%3B+dan+%CE%94Gi+%3D+104%2C331+kJ%2Fmol-1.+Enzim+hasil+modifikasi+kimia+menggunakan+asam+glioksilat+dengan+konsentrasi+1%2C5%3B+2%2C0%3B+dan+2%2C5+mg%2FmL+optimum+pada+pH+5%2C5+dan+suhu+55%EF%82%B0C+serta+memiliki+nilai+KM+berturut-turut+sebesar+16%2C74%3B+16%2C28%3B+dan+14%2C67+mg%2FmL-1+substrat%2C+nilai+Vmaks+berturut-turut+sebesar+9%2C32%3B+7%2C92%3B+dan+7%2C07+%CE%BCmol+mL-1menit-1serta+uji+stabilitas+termal+enzim+selama+100+menit+pada+suhu+60oC+memiliki+aktivitas+sisa+berturut-turut+sebesar+28%2C59%3B+35%2C93%3B+dan+39%2C23%25+serta+memiliki+nilai+ki+%3D+0%2C0105%3B+0%2C0096%3B+dan+0%2C0088+menit-1%2C+nilai+t1%2F2+%3D+66%2C00%3B+72%2C19%3B+dan+78%2C75+menit%2C+dan+nilai+%CE%94Gi+%3D+105%2C799%3B+106%2C076%3B+dan+106%2C297+kJ%2Fmol-1.+Enzim+hasil+modifikasi+kimia+dengan+asam+glioksilat+memiliki+kestabilan+termal+yang+tinggi%2C+hal+ini+dibuktikan+dengan+peningkatan+waktu+paruh+sebesar+1%2C08-2%2C05+kali+lipat+dibandingkan+dengan+enzim+murni.%0D%0AKata+kunci%3A+%CE%B1-amilase%2C+A.+fumigatus%2C+modifikasi+kimia%2C+asam+glioksilat.%0D%0A%0D%0A%0D%0A%CE%B1-Amylase+is+an+extracellular+enzyme+that+may+breakdown+1.4-D-glycosidic+bonds+between+glucose+monomers+in+amylose+linear+chains.+The+%CE%B1-amylase+enzyme+is+widely+used+in+a+variety+of+industrial+areas.+The+purpose+of+this+study+was+to+enhance+the+stability+of+the+%CE%B1-amylase+enzyme+from+A.+fumigatus+by+chemical+modification+using+glyoxylic+acid.+The+%CE%B1-amylase+enzyme+was+isolated+by+centrifugation+and+purified+by+ammonium+sulfate+fractionation+and+dialysis.+The+purified+enzyme+was+modified+with+glyoxylic+acid.+%CE%B1-Amylase+activity+was+tested+using+the+Fuwa+method+and+Mandels+method.+Protein+content+was+determined+by+the+Lowry+method.+The+results+showed+that+the+specific+activity+of+the+purified+enzyme%2C+1%2C037.42+U%2Fmg%2C+increased+18+times+over+the+crude+extract+of+the+enzyme%2C+which+had+a+specific+activity+of+58.31+U%2Fmg.+The+purified+enzyme+has+an+optimum+pH+of+5.0+with+an+optimum+temperature+of+55%C2%B0C%3B+KM+%3D+11.55+mg%2FmL-1+substrate%2C+Vmax+%3D+10.76+mole%2FmL-1+and+the+thermal+stability+test+at+60%C2%B0C+for+100+minutes+yielded+a+residual+activity+of+9.34%25+with+ki+%3D+0.018+minutes-1%2C+t1%2F2+%3D+38.50+minutes%2C+and+%CE%94Gi+%3D+104.331+kJ%2Fmol-1.+Chemical+modification+enzyme+using+glyoxylic+acid+with+a+concentration+of+1.5%3B+2.0%3B+and+2.5+mg%2FmL+optimum+pH+at+5.5+and+temperature+55%C2%B0C+and+has+a+KM+value+of+16.74%3B+16.28%3B+and+14.67+mg%2FmL-1+substrate%2C+Vmax+value+are+9.32%3B+7.92%3B+and+7.07+mole%2FmL-1+and+the+thermal+stability+test+at+60%C2%B0C+for+100+minutes+yielded+residual+activity+of+28.59%2C+35.93%2C+and+39.23%25%2C+with+ki+%3D+0.0105%2C+0.0096%2C+and+0.0088+min-1%2C+t1%2F2+%3D+66.00%2C+72.19%2C+and+78.75+minutes%2C+and+%CE%94Gi+%3D+105.799%2C+106.076%2C+and+106.297+kJ%2Fmole-1.+Enzymes+that+have+been+chemically+modified+with+glyoxylic+acid+show+great+thermal+stability%2C+as+indicated+by+a+1.08-2.05-fold+increase+in+half-life+when+compared+to+purified+enzymes.%0D%0AKeywords%3A+%CE%B1-amylase%2C+A.+fumigatus%2C+chemical+modification%2C+glyoxylic+acid.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2022-12-05&rft.type=Skripsi&rft.ty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