?url_ver=Z39.88-2004&rft_id=1817011066&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=PENGARUH+MODIFIKASI+KIMIA+MENGGUNAKAN+SITRAKONAT%0D%0AANHIDRIDA+TERHADAP+STABILITAS+ENZIM+%CE%B1-AMILASE%0D%0ADARI+Aspergillus+fumigatus&rft.creator=Lupia+Widya+Astuti+%2C+lupiawidya22%40gmail.com&rft.subject=000+Ilmu+komputer%2C+informasi+dan+pekerjaan+umum&rft.subject=001+Ilmu+pengetahuan&rft.description=ABSTRAK%0D%0APENGARUH+MODIFIKASI+KIMIA+MENGGUNAKAN+SITRAKONAT%0D%0AANHIDRIDA+TERHADAP+STABILITAS+ENZIM+%CE%B1-AMILASE%0D%0ADARI+Aspergillus+fumigatus%0D%0AOleh%0D%0ALupia+Widya+Astuti%0D%0AKestabilan+enzim+dalam+industri+merupakan+faktor+penting%2C+karena+enzim%0D%0Aumumnya+tidak+stabil+pada+pH+dan+suhu+ekstrim.+Penelitian+ini+bertujuan+untuk%0D%0Ameningkatkan+stabilitas+enzim+%CE%B1-amilase+dari+A.+fumigatus+dengan+modifikasi+kimia%0D%0Amenggunakan+sitrakonat+anhidrida.+Proses+penelitian+dilakukan+melalui+tahapan%0D%0Aberikut%3A+produksi+enzim%2C+isolasi%2C+pemurnian%2C+modifikasi+kimia+dan+karakterisasi.%0D%0AAktivitas+enzim+ditentukan+dengan+metode+Fuwa+dan+Mandels+serta+kadar+protein%0D%0Adengan+metode+Lowry.+Hasil+penelitian+menunjukkan+enzim+hasil+pemurnian%0D%0Amempunyai+pH+optimum+5%3B+suhu+optimum+50%C2%B0C%3B+KM+%3D+3%2C01+mg%2FmL+substrat%3B+Vmaks%0D%0A%3D+35%2C46+%CE%BCmol%2FmL.menit%2C+dan+uji+stabilitas+termal+pada+suhu+60%C2%B0C+selama+100+menit%0D%0Amenunjukkan+nilai+ki+%3D+0%2C0179+menit-1%3B+t1%2F2+%3D+38%2C72+menit%3B+dan+%CE%94Gi+%3D+104%2C26+kJ%2Fmol.%0D%0AEnzim+hasil+modifikasi+menggunakan+sitrakonat+anhidrida+40+%CE%BCL+memiliki+pH%0D%0Aoptimum+6+sedangkan+50+%CE%BCL+dan+60+%CE%BCL+memiliki+pH+optimum+5%2C5+dengan+suhu%0D%0Aoptimum+ketiga+penambahan+tersebut+adalah+60%C2%BAC%3B+nilai+KM+berturut-turut+adalah%0D%0A4%2C57%3B+5%2C03%3B+dan+5%2C22+mg%2FmL+substrat%3B+nilai+Vmaks+adalah+27%2C70%3B+25%2C77%3B+dan+25%2C71%0D%0A%CE%BCmol%2FmL+menit.+Uji+stabilitas+termal+enzim+hasil+modifikasi+menggunakan%0D%0Asitrakonat+anhidrida+40%2C+50%2C+dan+60+%CE%BCL+pada+suhu+60%C2%B0C+selama+100+menit+secara%0D%0Aberturut-turut+memiliki+nilai+ki+sebesar+0%2C0027%3B+0%2C0023%3B+dan+0%2C0021+menit-1%3B+t1%2F2%0D%0Asebesar+256%2C67%3B+301%2C30%3B+dan+330%2C00+menit%3B+dan+nilai+%CE%94Gi+sebesar+109%2C584%3B+110%2C028%3B%0D%0Adan+110%2C279+kJ%2Fmol.+Modifikasi+kimia+enzim+%CE%B1-amilase+dari+jamur+A.+fumigatus%0D%0Amenggunakan+sitrakonat+anhidrida+dapat+meningkatkan+stabilitas+termal+sebesar%0D%0A6%2C6-8%2C5+kali.+Hal+ini+dibuktikan+dengan+penurunan+nilai+ki+dan+peningkatan+nilai+t1%2F2%0D%0Adan+%CE%94Gi.%0D%0AKata+kunci%3A+%CE%B1-amilase%2C+Aspergillus+fumigatus%2C+modifikasi+kimia%2C+sitrakonat%0D%0Aanhidrida%0D%0A%0D%0A%0D%0AThe+stability+of+enzymes+for+industrial+usage+is+important+because+enzymes+are%0D%0Agenerally+unstable+at+high+pH+and+temperatures.+This+work+aims+to+improve+the%0D%0Astability+of+the+%CE%B1-amylase+enzyme+from+A.+fumigatus+by+chemical+modification%0D%0Ausing+citraconic+anhydride.+The+following+phases+were+followed+in+the+research%0D%0Aprocess%3A+enzyme+production%2C+isolation%2C+purification%2C+chemical+modification%2C+and%0D%0Acharacterization.+The+enzyme+activity+was+then+evaluated+using+the+Fuwa+and%0D%0AMandels+methods%2C+and+the+protein+content+was+assessed+using+the+Lowry+method.%0D%0AThe+enzyme+activity+was+then+evaluated+using+the+Fuwa+and+Mandels+methods%2C+and%0D%0Athe+protein+content+was+assessed+using+the+Lowry+method.+The+results+showed+that%0D%0Athe+native+enzymes+had+an+optimum+pH+of+5%3B+optimum+temperature+50%C2%B0C%3B+KM+%3D%0D%0A3.01+mg%2FmL+substrate%3B+Vmax+%3D+35.46+%CE%BCmol%2FmL.min%2C+ki+%3D+0.0179+min-1%3B+t1%2F2+%3D+38.72%0D%0Amin%3B+dan+%CE%94Gi+%3D+104.26+kJ%2Fmole.+The+modified+enzyme+using+citraconic+anhydride%0D%0A40+%CE%BCL+has+an+optimum+pH+of+6+while+50+%CE%BCL+and+60+%CE%BCL+has+an+optimum+pH+of+5.5%0D%0Aand+the+optimum+temperature+of+three+concentrations+is+60%C2%BAC%3B+KM+%3D+4.57%2C+5.03%2C+and%0D%0A5.22+mg%2FmL+substrate%3B+Vmax+%3D+27.70%2C+25.77%2C+and+25.71+%CE%BCmol%2FmL.min%2C+respectively.%0D%0AThe+modified+enzyme+thermal+stability+test+with+citraconic+anhydride+40%2C+50%2C+and%0D%0A60+%CE%BCL+at+60%C2%B0C+for+100+minutes+yielded+ki+values+of+0.0027%2C+0.0023%2C+and+0.0021+min-%0D%0A1%2C+t1%2F2+values+of+256.67%2C+301.30%2C+and+330.00+min%2C+and+%CE%94Gi+values+of+109.58%2C+110.03%2C%0D%0Aand+110.28+kJ%2Fmole.+Chemical+modification+of+the+%CE%B1-amylase+enzyme+from+the%0D%0Afungus+A.+fumigatus+with+citraconic+anhydride+can+increase+thermal+stability+by+6.6-%0D%0A8.5+times.+This+is+seen+by+a+decrease+in+the+value+of+ki+and+increasing+the+values+of%0D%0At1%2F2+and+%CE%94Gi.%0D%0AKeywords%3A+%CE%B1-amylase%2C+Aspergillus+fumigatus%2C+chemical+modification%2C%0D%0Acitraconic+anhydride&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2022-12-06&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F68069%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib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