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<http://digilib.unila.ac.id/id/eprint/68088> <http://purl.org/dc/terms/title> "VALIDASI METODE UJI AKTIVITAS ANTIOKSIDAN PADA\r\nEKSTRAK ETANOL KULIT BUAH KAKAO DAN KULIT BUAH NANAS\r\nMENGGUNAKAN METODE DPPH (1,1-DIFENIL-2-PIKRILHIDRAZIL)\r\nSECARA SPEKTROFOTOMETRI UV-VIS"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/eprint/68088> <http://purl.org/ontology/bibo/abstract> "Penelitian validasi metode uji aktivitas antioksidan pada ekstrak etanol kulit kakao\r\ndan kulit nanas menggunakan metode DPPH secara spektrofotometri UV-Vis\r\ntelah dilakukan. Penelitian ini bertujuan untuk melakukan validasi metode uji\r\naktivitas antioksidan, menghitung ketidakpastian pengukuran, serta melakukan uji\r\naktivitas antioksidan pada kulit kakao dan nanas. Sampel dipreparasi dengan cara\r\ndimaserasi menggunakan pelarut etanol 70%. Pengujian aktivitas antioksidan\r\ndilakukan dengan membuat variasi konsentrasi sampel lalu ditambahkan larutan\r\nDPPH sebanyak 1 mL dan diinkubasi selama 30 menit, absorbansi diukur pada\r\npanjang gelombang 517 nm. Kemudian dilakukan validasi metode dan estimasi\r\nketidakpastian pengukuran. Hasil penelitian menunjukkan uji aktivitas antioksidan\r\nmenggunakan metode DPPH secara Spektrofotometri UV-Vis merupakan metode\r\nvalid. Berdasarkan hasil uji linearitas dengan nilai R2= 0,9916, uji akurasi sampel\r\nkulit kakao diperoleh nilai % recovery pada sampel 200; 600; 1000 ppm ditambah\r\n4 ppm standar yaitu masing-masing 99,30%; 101,43% dan 101,66%. Uji akurasi\r\nsampel kulit nanas diperoleh % recovery pada sampel 9,08; 10,23; 12,04 ppm\r\nditambah 4 ppm standar yaitu masing-masing 98,12%; 99,17% dan 101,93%. %\r\nRSD dari uji presisi sampel kulit kakao 200; 600; 1000 ppm yaitu masing-masing\r\n1,60%; 1,91% dan 1,29%. % RSD dari uji presisi sampel kulit nanas 9,08; 10,23;\r\n12,04 ppm yaitu masing-masing 0,99%; 0,89% dan 0,95%. Aktivitas antioksidan\r\nsampel kulit kakao yang diperoleh yaitu 1134,00 ppm dengan nilai ketidakpastian\r\n1,34 dan Aktivitas antioksidan sampel kulit nanas yang diperoleh sebesar 7,24\r\nppm dengan nilai ketidakpastian pengukuran 1,70. Nilai LoQ dan LoD yang\r\ndiperoleh yaitu 27,91 ppm dan 93,06 ppm.\r\nKata kunci: Kulit kakao, kulit nanas, aktivitas antioksidan, DPPH, validasi."^^<http://www.w3.org/2001/XMLSchema#string> .
<http://digilib.unila.ac.id/id/eprint/68088> <http://purl.org/dc/terms/date> "2022-12-19" .
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<http://digilib.unila.ac.id/id/document/2090766> <http://eprints.org/relation/isVersionOf> <http://digilib.unila.ac.id/id/document/2090758> .
<http://digilib.unila.ac.id/id/document/2090766> <http://eprints.org/relation/isVolatileVersionOf> <http://digilib.unila.ac.id/id/document/2090758> .
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<http://digilib.unila.ac.id/id/document/2090767> <http://eprints.org/relation/isVolatileVersionOf> <http://digilib.unila.ac.id/id/document/2090758> .
<http://digilib.unila.ac.id/id/document/2090767> <http://eprints.org/relation/ispreviewThumbnailVersionOf> <http://digilib.unila.ac.id/id/document/2090758> .
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<http://digilib.unila.ac.id/id/document/2090767> <http://purl.org/dc/terms/hasPart> <http://digilib.unila.ac.id/68088/12/preview.jpg> .
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<http://digilib.unila.ac.id/id/document/2090768> <http://eprints.org/relation/isVolatileVersionOf> <http://digilib.unila.ac.id/id/document/2090758> .
<http://digilib.unila.ac.id/id/document/2090768> <http://eprints.org/relation/ismediumThumbnailVersionOf> <http://digilib.unila.ac.id/id/document/2090758> .
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<http://digilib.unila.ac.id/68088/> <http://xmlns.com/foaf/0.1/primaryTopic> <http://digilib.unila.ac.id/id/eprint/68088> .
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