?url_ver=Z39.88-2004&rft_id=1817011021&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=PENINGKATAN+KESTABILAN+ENZIM+%CE%B1-AMILASE+DARI%0D%0AAspergillus+fumigatus+DENGAN+PENAMBAHAN%0D%0APOLIETILEN+GLIKOL+(PEG)+6000&rft.creator=Eka+Candra+Wati%2C+1817011021&rft.subject=000+Ilmu+komputer%2C+informasi+dan+pekerjaan+umum&rft.description=Enzim+banyak+digunakan+dalam+kegiatan+industri%2C+namun+untuk+industri+di+Indonesia+kebutuhan+enzim+belum+dapat+terpenuhi+sehingga+harus+mengimpor+enzim.+Salah+satu+enzim+yang+memiliki+peran+penting+ialah+enzim+%CE%B1-amilase.+Penelitian+ini+bertujuan+untuk+meningkatkan+kestabilan+enzim+%CE%B1-amilase+dari+A.+fumigatus+dengan+cara+penambahan+polietilen+glikol+(PEG)+6000.+Tahapan+penelitian+yang+dilakukan+yaitu%3A+produksi%2C+isolasi%2C+pemurnian%2C+dan+karakterisasi.+Aktivitas+enzim+%CE%B1-amilase+ditentukan+menggunakan+metode+Fuwa+dan+Mandels%2C+serta+kadar+protein+ditentukan+dengan+metode+Lowry.+Hasil+penelitian+menunjukkan+aktivitas+spesifik+enzim+hasil+pemurnian+sebesar+753%2C83+U%2Fmg+yang+mengalami+peningkatan+kemurnian+15+kali+dibandingkan+dengan+ekstrak+kasarnya+51%2C97+U%2Fmg.+Enzim+hasil+pemurnian+mempunyai+suhu+optimum+50%C2%B0C+dan+pH+optimum+5%3B+uji+stabilitas+termal+pada+suhu+50%C2%B0C+mempunyai+nilai+ki+%3D+0%2C0389+%C2%B1+0%2C0001+menit-1%3B+t1%2F2+%3D+17%2C42+%C2%B1+0%2C06+menit%3B+%CE%94Gi+%3D+102%2C136+%C2%B1+0%2C003+kJ+mol-1.+Enzim+setelah+penambahan+PEG+6000+konsentrasi+20%2C+25%2C+dan+30%25+memiliki+suhu+optimum+55%C2%BAC+dan+pH+optimum+6%3B+uji+stabilitas+termal+enzim+setelah+penambahan+PEG+6000+konsentrasi+20%2C+25%2C+dan+30%25+pada+suhu+55%C2%B0C+mempunyai+nilai+ki+%3D+0%2C0058+%C2%B1+0%2C0000%3B+0%2C0076+%C2%B1+0%2C0001%3B+dan+0%2C0077+%C2%B1+0%2C0001+menit-1%3B+t1%2F2+%3D+119%2C51+%C2%B1+0%2C00%3B+90%2C61+%C2%B1+0%2C84%3B+dan+90%2C03+%C2%B1+1%2C65+menit%3B+dan+nilai+%CE%94Gi+%3D+107%2C468+%C2%B1+0%2C00%3B+106%2C702+%C2%B1+0%2C01%3B+dan+106%2C684+%C2%B1+0%2C02+kJ+mol-1.+Penambahan+PEG+6000+dapat+meningkatkan+kestabilan+enzim+%CE%B1-amilase+dari+A.+fumigatus+6%2C86+%C2%B1+0%2C02+kali+dibandingkan+enzim+hasil+pemurnian%2C+ditunjukkan+dengan+terjadinya+penurunan+nilai+ki%2C+peningkatan+t1%2F2+dan+%CE%94Gi+dari+enzim+setelah+penambahan+PEG+6000.%0D%0A%0D%0AKata+kunci%3A+%CE%B1-amilase%2C+PEG+6000%2C+kestabilan+enzim%2C+dan+A.+fumigatus%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0Aabstract%0D%0A%0D%0AEnzymes+are+widely+used+in+industrial+activities%2C+but+for+industry+in+Indonesia+the+need+for+enzymes+can%E2%80%99t+be+fulfilled%2C+so+they+have+to+import+enzymes.+One+of+the+enzymes+that+have+an+important+role+is+the+%CE%B1-amylase.+This+study+aims+to+increase+the+stability+of+the+%CE%B1-amylase+enzyme+from+A.+fumigatus+by+adding+polyethylene+glycol+(PEG)+6000.+The+stages+of+the+research+carried+out+were+the+production%2C+isolation%2C+purification%2C+and+characterization.+The+%CE%B1-amylase+enzyme+activity+was+evaluated+using+the+Fuwa+and+Mandels+method%2C+and+the+protein+content+was+evaluated+using+the+Lowry+method.+The+results+of+the+research+showed+the+specific+activity+of+the+purified+enzyme+was+753.83+U%2Fmg+which+increased+15+times+in+purity+compared+to+the+crude+extract%2C+which+was+51.97+U%2Fmg.+The+purified+enzyme+has+an+optimum+temperature+of+50%C2%B0C+and+an+optimum+pH+of+5%2C+test+the+thermal+stability+at+50%C2%B0C+has+a+value+of+ki+%3D+0.0389+min-1+%C2%B1+0.0001%3B+t1%2F2+%3D+17.42+%C2%B1+0.06+min%3B+%CE%94Gi+%3D+102.136+%C2%B1+0.003+kJ+mole-1.+Enzymes+after+the+additionof+PEG+6000+concentration+of+20%2C+25%2C+and+30%25+have+an+optimum+temperature+of+55%C2%B0C+and+optimum+pH+of+6%3B+test+the+thermal+stability+of+the+enzyme+after+addition+of+PEG+6000+concentration+of+20%2C+25%2C+and+30%25+at+55%C2%B0C+has+a+value+of+ki+%3D+0.0058+%C2%B1+0.0000%3B+0.0076+%C2%B1+0.0001%3B+and+0.0077+%C2%B1+0.0001+min-1%3B+t1%2F2+%3D+119.51+%C2%B1+0.00%3B+90.61+%C2%B1+0.84%3B+and+90.03+%C2%B1+1.65+min%3B+and+value+%CE%94Gi+%3D+107.468+%C2%B1+0.00%3B+106.702+%C2%B1+0.01%3B+and+106.684+%C2%B1+0.02+kJ+mol-1.+The+addition+of+PEG+6000+was+able+to+increase+the+stability+of+the+%CE%B1-amylase+from+A.+fumigatus+6.86+%C2%B1+0.02+times+compared+to+the+purified+enzymes%2C+indicated+by+a+decrease+in+the+ki+value%2C+an+increase+in+the+t1%2F2+and+%CE%94Gi+of+the+enzyme+after+the+addition+of+PEG+6000.%0D%0A%0D%0AKeywords%3A+%CE%B1-amylase%2C+PEG+6000%2C+enzyme+stability%2C+and+A.+fumigatus&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2022-12-05&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F68108%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F68108%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F68108%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++Eka+Candra+Wati%2C+1817011021++(2022)+PENINGKATAN+KESTABILAN+ENZIM+%CE%B1-AMILASE+DARI+Aspergillus+fumigatus+DENGAN+PENAMBAHAN+POLIETILEN+GLIKOL+(PEG)+60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