?url_ver=Z39.88-2004&rft_id=1817011074&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=KARAKTERISASI+EKSTRAK+CHITOOLIGOSACCHARIDE+HASIL+DEGRADASI+KULIT+UDANG+MENGGUNAKAN+AKTINOMISETES+SEBAGAI+ANTIFUNGI+TERHADAP+Malassezia+globosa&rft.creator=Chasya+Al-Afandy%2C+1817011074&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.description=Chitooligosaccharide+(COS)+merupakan+salah+satu+oligomer+yang+dihasilkan+dari+degradasi+kitosan+atau+kitin.+COS+memiliki+kelarutan+yang+baik+dalam+air+karena+panjang+rantainya+yang+lebih+pendek+dan+gugus+asam+amino+bebas+dalam+unit+D-glukosamin.+COS+telah+diketahui+memiliki+aktivitas+sebagai+antifungi.+COS+dapat+diproduksi+dengan+beberapa+metode+seperti+kimia%2C+fisik+serta+biologi.+Produksi+COS+dengan+cara+biologis+menggunkan+enzim+memiliki+banyak+keuntungan+dibandingkan+dengan+metode+fisik+dan+kimia%2C+dan+pada+penelitian+ini+menggunakan+aktinomisetes+sebagai+alternatif+penggunaan+enzim%2C+karena+aktinomisetes+dapat+menghasilkan+enzim+kitinase.+Tujuan+dilakukannya+penelitian+ini+adalah%2C+untuk+memperoleh+isolat+aktinomisetes+yang+dapat+mendegradasi+kulit+udang%2C+memperoleh+isolat+aktinomisetes+yang+mampu+mengisolasi+COS+dari+hasil+degradasi+kulit+udang+menggunakan+teknik+SSF%2C+mengkarakterisasi+dan+memfraksinasi+COS+hasil+degradasi+dari+limbah+kulit+udang%2C+serta+menguji+aktivitas+antifungi+senyawa+COS.+Berdasarkan+penelitian+didapatkan+hasil%2C+dimana+isolat+dengan+kode+18D36A1%2C+18D36A2%2C+19A07A1%2C+19B19A1%2C+19B20A1%2C+19C34A1+dan+19C38A1+memiliki+potensi+dalam+degradasi+kulit+udang.+Isolat+18D36A1%2C+18D36A2%2C+19A07A1%2C+19B19A1%2C+19B20A1%2C+19C34A1+dan+19C38A1+mampu+mendegradasi+kulit+udang+menjadi+COS+pada+hari+ke-6+yang+dibuktikan+dengan+KLT.+COS+hasil+degradasi+dari+isolat+18D36A1+mampu+menghambat+pertumbuhan+M.+globosa+pada+dosis+1mg%2FmL.+Serta%2C+pada+karakterisasi+produk+hasil+degradasi+18D36A1+menggunakan+FTIR+dan+LCMS-MS+terindikasi+merupakan+COS+yang+memiliki+derajat+plimerisasi+(DP)+6+dengan+pola+A4D2.%0D%0A%0D%0AKata+kunci%3A+kulit+udang%2C+aktinomisetes%2C+chitooligosaccharide%2C+antifungi%2C+Malassezia+globosa.%0D%0A%0D%0AChitooligosaccharide+(COS)+is+an+oligomer+produced+from+the+degradation+of+chitosan+or+chitin.+COS+has+good+solubility+in+water+because+it+has+a+shorter+chain+length+and+free+amino+acid+groups+in+the+D-glucosamine+unit.+COS+have+been+known+to+have+antifungal+activity.+COS+can+be+produced+by+using+chemical%2C+physical+as+well+as+biology.+Production+of+COS+by+biological+means+using+enzymes+has+many+advantages%2C+and+in+this+study+using+actinomycetes+as+an+alternative+to+using+enzymes%2C+because+actinomycetes+can+produce+chitinase+enzymes.+This+study+aims+to+obtain+actinomycetes+isolates+that+can+degrade+shrimp+shells%2C+obtain+actinomycetes+isolates+that+are+able+to+isolate+COS+from+shrimp+shell+degradation+using+the+SSF+technique%2C+characterize+COS+degradation+products+from+shrimp+shell+waste%2C+and+test+the+antifungal+activity+of+COS+compounds.+Based+on+the+results+of+the+research%2C+isolates+with+codes+18D36A1%2C+18D36A2%2C+19A07A1%2C+19B19A1%2C+19B20A1%2C+19C34A1+and+19C38A1+have+the+potential+to+degrade+shrimp+shells.+Isolates+18D36A1%2C+18D36A2%2C+19A07A1%2C+19B19A1%2C+19B20A1%2C+19C34A1+and+19C38A1+were+able+to+degrade+shrimp+shells+to+COS+on+the+6th+day+as+evidenced+by+TLC.+COS+resulting+from+the+degradation+of+Isolate+18D36A1+was+able+to+inhibit+the+growth+of+Malassezia+globosa+at+a+dose+of+1+mg%2FmL.+Also%2C+in+the+characterization+of+the+18D36A1+degradation+product+using+FTIR+and+LCMS-MS%2C+it+is+indicated+that+COS+has+a+degree+of+polimezation+(DP)+of+6+with+an+A4D2+pattern.%0D%0A%0D%0AKeywords%3A+shrimp+shell%2C+actinomycetes%2C+chitooligosaccharide%2C+antifungal%2C+Malassezia+globosa.&rft.publisher=Matematika+dan+Ilmu+Pengetahuan+Alam&rft.date=2022-12-14&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F68110%2F1%2F1.%2520ABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F68110%2F2%2F2.%2520FILE%2520SKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F68110%2F3%2F3.%2520FILE%2520SKRIPSI%2520FULL%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++Chasya+Al-Afandy%2C+1817011074++(2022)+KARAKTERISASI+EKSTRAK+CHITOOLIGOSACCHARIDE+HASIL+DEGRADASI+KULIT+UDANG+MENGGUNAKAN+AKTINOMISETES+SEBAGAI+ANTIFUNGI+TERHADAP+Malassezia+globosa.++Matematika+dan+Ilmu+Pengetahuan+Alam%2C+Universitas+Lampung.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F68110%2F