?url_ver=Z39.88-2004&rft_id=1817011027&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=UJI+BIOAKTIVITAS+EKSTRAK+METANOL+DARI+ACTINOMYCETES+SEBAGAI+ANTIBIOFILM+TERHADAP+Staphylococcus+aureus+&rft.creator=MEGA%2C+MURYANI&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.description=Senyawa+metabolit+sekunder+dari+Actinomycetes+menjadi+sumber+potensial+dalam+memperoleh+senyawa+bioaktif+yang+beragam.+Penelitian+ini+bertujuan+untuk+mendapatkan+eksrak+metanol+dari+Actinomycetes+yang+berpotensi+sebagai+antibiofilm+terhadap+S.+aureus+resisten.+Isolat+Actinomycetes+diinokulasi+pada+media+cair+koloid+kitin+1%25+menggunakan+air+laut+buatan.+Inokulum+Actinomycetes+kemudian+dikultivasi+pada+media+kulit+udang+selama+14+hari+dalam+keadaan+statis.+Ekstrak+kasar+Actinomycetes+yang+diperoleh+selanjutnya+dilakukan+uji+skrining+terkait+bioaktivitasnya.+Uji+skrining+antibakteri+menggunakan+metode+difusi%2C+ekstrak+sampel+Actinomycetes+tidak+menunjukkan+adanya+daya+hambat+terhadap+S.+aureus+resisten.+Lebih+lanjut%2C+uji+skrining+antibiofilm+dengan+metode+microtiter+96-well+plate+dievaluasi+melalui+pewarnaan+crystal+violet+yang+dianalisis+nilai+absorbansinya+untuk+memperoleh+persen+hambat+biofilm.+Isolat+19A07-A1+memiliki+potensi+sebagai+antibiofilm+tanpa+membunuh+S.+aureus+resisten.+Selanjutnya%2C+isolat+19A07-A1+dilakukan+pemurnian+dengan+MPLC+dan+karakterisasi+menggunakan+LC-MS%2FMS.+Dalam+penelitian+ini%2C+isolat+19A07-A1+memiliki+potensi+intermediet+yang+menghambat+biofilm+S.+aureus+pada+konsentrasi+250+%C2%B5g%2FmL.+Hasil+karakterisasi+menunjukkan+sifat+kimia+senyawa+isolat+19A07-A1+mengandung+adanya+senyawa+piridin.+Hasil+ini+menunjukkan+bahwa+Actinomycetes+yang+ditumbuhkan+pada+media+kulit+udang+terkait+isolat+19A07-A1+mampu+menghasilkan+senyawa+yang+berpotensi+sebagai+antibiofilm+terhadap+S.+aureus+resisten.+%0D%0A+%0D%0AKata+kunci%3A+Actinomycetes%2C+antibiofilm%2C+S.+aureus+resisten%2C+%0D%0Akulit+udang%2C+fermentasi+padat.+%0D%0A%0D%0ASecondary+metabolite+compounds+from+actinomycetes+are+a+potential+source+in+obtaining+various+bioactive+compounds.+This+study+aims+to+obtain+methanol+extract+from+actinomycete+which+has+the+potential+as+an+anti-biofilm+against+resistant+S.aureus.+Actinomycetes+isolates+were+inoculated+in+1%25+chitin+colloidal+liquid+medium+using+artificial+seawater.+The+actinomycetes+inoculum+was+then+cultivated+in+shrimp+shell+media+for+14+days+in+a+static+state.+The+actinomycetes+crude+extract+obtained+was+then+subjected+to+screening+test+related+to+its+bioactivity.+Antibacterial+screening+test+using+the+diffusion+method%2C+actinomycetes+sample+extract+did+not+show+any+inhibition+against+resistant+S.+aureus.+Furthermore%2C+the+anti-biofilm+screening+test+using+the+96-well+microtiter+plate+method+was+evaluated+by+means+of+crystal+violet+staining+and+the+absorbance+value+was+analyzed+to+obtain+the+percent+inhibition+of+the+biofilm.+Isolate+19A07A1+has+potential+as+an+anti-biofilm+without+killing+resistant+S.+aureus.+Furthermore%2C+isolate+19A07-A1+was+purified+using+MPLC+and+characterization+using+LC-MS%2FMS.+In+this+study%2C+isolate+19A07-A1+had+potential+intermediates+that+inhibited+S.+aureus+biofilms+at+a+concentration+of+250+%C2%B5g%2FmL.+The+characterization+results+showed+that+the+chemical+properties+of+isolate+19A07-A1+contained+the+presence+of+pyridine+compounds.+These+results+indicate+that+actinomycetes+grown+on+shrimp+shell+media+related+to+isolate+19A07-A1+is+capable+of+producing+compounds+that+have+potential+to+act+as+anti-biofilms+against+resistant+S.+aureus.+%0D%0A+%0D%0AKeywords%3A+Actinomycetes%2C+anti-biofilm%2C+resistant+S.+aureus%2C+shrimp+shells%2C+solid+state+fermentation+%0D%0A%0D%0A&rft.publisher=MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2022-12-27&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F68558%2F1%2F1.%2520ABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F68558%2F2%2F2.%2520FILE%2520SKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F68558%2F3%2F3.%2520FILE%2520SKRIPSI%2520FULL%2520TANPA%2520PEMBAHASAN.pdf&rft.identifier=++MEGA%2C+MURYANI++(2022)+UJI+BIOAKTIVITAS+EKSTRAK+METANOL+DARI+ACTINOMYCETES+SEBAGAI+ANTIBIOFILM+TERHADAP+Staphylococcus+aureus.++MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F68558%2F