?url_ver=Z39.88-2004&rft_id=1817011029&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=OPTIMASI+PRODUKSI+HIDROLISAT+PROTEIN+DARI+LIMBAH+BULU+AYAM+MENGGUNAKAN+BAKTERI+ISOLAT+LOKAL+B-2-2&rft.creator=Aprilia+Fransiska+Br+Sembiring%2C+1817011029&rft.subject=000+Ilmu+komputer%2C+informasi+dan+pekerjaan+umum&rft.description=Limbah+bulu+ayam+merupakan+salah+satu+limbah+yang+sulit+terdegradasi+karena+kandungan+protein+keratin+yang+terkandung+di+dalamnya.+Keratin+yang+terdapat+pada+bulu+ayam+memiliki+sifat+yang+sulit+terdegradasi+karena+memiliki+stabilitas+ikatan+yang+tinggi+dan+sulit+larut+dalam+air.+Biodegradasi+oleh+mikroba+untuk+mengubah+struktur+komplek+keratin+dipilih+karena+prosesnya+lebih+ramah+lingkungan.+Isolat+B-2-2+sebelumnya+dilaporkan+memiliki+mampu+mendegradasi+68%25+limbah+bulu+ayam+dalam+medium+FML+dengan+sumber+C+glukosa+pada+pH+9+selama+12+hari+dan+menghasilkan+hidrolisat+protein+sebesar+801+ppm%2C+kemampuannya+masih+perlu+diselidiki+lebih+lanjut+untuk+mendapatkan+hasil+yang+lebih+maksimal.+Penelitian+ini+bertujuan+untuk+menyelidiki+kondisi+kultur+optimum+menggunakan+sumber+karbon+(glukosa%2C+laktosa+dan+sukrosa)+dan+sumber+nitrogen+(NH4Cl%2C+NaNO3%2C+dan+KNO3)%2C+mengetahui+kadar+asam+amino+yang+dibebaskan%2C+dan+kadar+hidrolisat+protein+selama+proses+biodegradasi.+Uji+biodegradasi+oleh+isolat+ini+dilakukan+pada+medium+FML+menggunakan+variasi+inokulum+(5%2C+10+dan+20+mL)+dan+1%25+sumber+karbon+dan+nitrogen+selama+12+hari.+Data+yang+diamati+dari+penelitian+ini+adalah+degradasi+bulu+ayam%2C+aktivitas+enzim%2C+asam+amino+yang+dibebaskan%2C+dan+kadar+hidrolisat+protein+pada+kultur.+Hasil+penelitian+menunjukkan+bahwa+isolat+B-2-2+mampu+mendegradasi+71%2C54%25+bulu+ayam+dengan+volume+inokulum10+mL+pada+medium+FML+tanpa+penambahan+sumber+C+atau+sumber+N+lain.+Pada+kondisi+tersebut+diperoleh+aktivitas+enzim+optimum+diperoleh+pada+hari+ke-8+sebesar+42+U%2FmL%2C+kadar+asam+amino+yang+dibebaskan+tertinggi+pada+hari+ke-12+sebesar+691+ppm%2C+dan+kadar+hidrolisat+protein+pada+kultur+optimum+pada+hari+ke-5+sebesar+536+ppm.+Dari+data-data+tersebut+membuktikan+bahwa+optimasi+medium+FML+dengan+penambahan+sumber+C+dan+sumber+N+pada+penelitian+ini+lebih+tidak+berpengaruh+terhadap+proses+degradasi+dan+juga+produksi+hidrolisat+protein.%0D%0A%0D%0AKata+kunci%3A+limbah+bulu+ayam%2C+keratin%2C+biodegradasi%2C+isolat+B-2-2%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0Aabstract%0D%0A%0D%0AChicken+feather+waste+is+challenging+to+degrade+due+to+its+keratin+protein+content.+Keratin+found+in+chicken+feathers+has+properties+that+are+difficult+to+be+degrade+because+its+high+bond+stability+and+difficult+to+dissolve+in+water.+Biodegradation+by+microbesto+change+the+complx+structure+of+keratin+was+chosen+because+the+process+is+more+environmentally+friendly.+Isolate+B-2-2%2C+was+previously+reported+to+degrade+68%25+of+chicken+feather+waste+in+FML+medium+with+C-source+glucose+at+pH+9+for+12+days+and+protein+hydrolysate+of+801+ppm%2C+its+ability+still+needs+to+be+investigated+further+to+get+maximum+result.+This+study+aimed+to+examine+the+optimum+culture+conditions+using+carbon+sources+(glucose%2C+lactose+and+sucrose)+and+nitrogen+sources+(NH4Cl%2C+NaNO3%2C+and+KNO3)%2C+determine+the+levels+of+amino+acids+liberated%2C+and+the+levels+of+protein+hydrolysates+during+the+biodegradation+process.+The+biodegradation+test+by+this+isolate+was+carried+out+on+FML+medium+using+a+variety+of+inoculums+(5%2C+10+and+20+mL)+and+1%25+carbon+and+nitrogen+sources+for+12+days.+The+data+observed+from+this+study+were+chicken+feather+degradation%2C+enzyme+activity%2C+amino+acids+liberated%2C+and+protein+hydrolysate+content+of+the+culture.+The+results+of+showed+that+isolate+B-2-2+was+able+to+degrade+71.54%25+of+chicken+feathers+with+an+inoculum+volume+of+10+mL+FML+medium+without+the+addition+of+C+or+N+sources.+Under+these+conditions%2C+the+optimum+enzyme+activity+was+obtained+on+day+8+at+42+U%2FmL%2C+the+highest+amino+acid+liberated+content+on+day+12+was+691+ppm%2C+and+the+optimum+culture+protein+hydrolysate+on+day+5+at+536+ppm.+These+data+prove+that+the+optimazion+of+FML+medium+with+the+addition+of+C+and+N+sources+in+this+study+has+less+effect+on+the+degradation+process+and+the+production+of+protein+hydrolysate.%0D%0A%0D%0AKeywords%3A+chicken+feather+waste%2C+keratins%2C+biodegradation%2C+isolate+B-2-2&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2023-02-08&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F70197%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F70197%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F70197%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++Aprilia+Fransiska+Br+Sembiring%2C+1817011029++(2023)+OPTIMASI+PRODUKSI+HIDROLISAT+PROTEIN+DARI+LIMBAH+BULU+AYAM+MENGGUNAKAN+BAKTERI+ISOLAT+LOKAL+B-2-2.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F70197%2F