TY  - GEN
CY  - UNIVERSITAS LAMPUNG
ID  - eprints70915
UR  - http://digilib.unila.ac.id/70915/
A1  - Titania , Dwi Amarta Putri 
Y1  - 2023/02/14/
N2  - 
Diabetes mellitus is a disease caused by metabolic disorders that occur in the
pancreas, characterised by hyperglycaemia. One alternative to control blood
glucose levels is to inhibit the performance of ?-amylase and ?-glucosidase
enzymes. The purpose of this study was to determine the effect of gallic acid
concentration and to determine the best gallic acid concentration conjugated to
corn starch using the Free Radical Grafting method on the inhibition of ?-amylase
and ?-glucosidase enzyme activity. This study consisted of the process of
conjugating corn starch and gallic acid with Free Radical Grafting and then testing
the total phenol and antioxidant activities. The research was arranged in a nonfactorial
Randomized Completed Block Design (RCBD). The study used 5
treatments with the addition of gallic acid concentrations P1 (0%); P2 (0.5%); P3
(1%); P4 (1.5%) and P5 (2%) and 4 replicates. The grafting results were analysed
for total phenolics, inhibition of ?-amylase and ?-glucosidase enzyme activities.
The data obtained were tested for data homogeneity, analysed for variance, and
then subjected to a further test of Least Significant Difference (LSD) at the 5%
level. The results showed that the best treatment was found at 2% gallic acid
concentration per weight of corn starch with a total phenol value of 62,97 ppm
(GAE), inhibition of ?-amylase enzyme activity of 24.75 mg/dL and ?-
glucosidase 96.59%.
Keywords: Gallic acid, free radical grafting, corn starch, ?-amylase, ?-
glucosidase.


Diabetes melitus merupakan penyakit yang disebabkan oleh gangguan
metabolisme yang terjadi pada organ pankreas yang ditandai dengan
hiperglikemia. Salah satu alternatif untuk mengendalikan kadar glukosa darah
adalah dengan menghambat kinerja enzim ?-amilase dan ?-glukosidase. Tujuan
penelitian ini adalah mengetahui pengaruh konsentrasi asam galat serta
mengetahui konsentrasi asam galat terbaik yang dikonjugasikan pada pati jagung
menggunakan metode Free Radical Grafting terhadap penghambatan aktivitas
enzim ?-amilase dan ?-glukosidase. Penelitian ini terdiri dari, tahapan proses
konjugat pati jagung dan asam galat dengan Free Radical Grafting kemudian
dilakukan uji aktivitas total fenol dan antioksidan. Penelitian disusun dalam
Rancangan Acak Kelompok Lengkap (RAKL) non faktorial. Penelitian
menggunakan 5 perlakuan dengan penambahan asam galat konsentrasi P1 (0%);
P2 (0,5%); P3 (1%); P4 (1,5%) dan P5 (2%) dan 4 ulangan. Hasil grafting
dianalisis total fenol, penghambatan aktivitas enzim ?-amilase dan ?-glukosidase.
Data yang diperoleh diuji kehomogenan data, dianalisis ragam, kemudian
dilakukan uji lanjut Beda Nyata Terkecil (BNT) taraf %5. Hasil penelitian
menunjukkan bahwa perlakuan terbaik terdapat pada konsentrasi asam galat 2%
per berat pati jagung dengan nilai total fenol 62,97 ppm (GAE), penghambatan
aktivitas enzim ?-amilase 24.75 mg/dL dan ?-glukosidase 96,59%.
Kata Kunci : Asam galat, free radical grafting, , pati jagung, ?-amilase, ?-
glukosidase
PB  - FAKULTAS PERTANIAN
TI  - PENGARUH MODIFIKASI PATI JAGUNG DENGAN METODE FREE
RADICAL GRAFTING (FRG) MENGGUNAKAN ASAM GALAT
TERHADAP PENGHAMBATAN AKTIVITAS ENZIM -AMILASE DAN
ENZIM -GLUKOSIDASE
AV  - restricted
ER  -