?url_ver=Z39.88-2004&rft_id=1817011045&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=PENGOPTIMALAN+KONDISI+AKTIVITAS+TRANSESTERIFIKASI+LIPASE+DARI+BAKTERI+Klebsiella+sp.+UNTUK+PRODUKSI%0D%0ABIODIESEL&rft.creator=Risna+i%2C+Milenia+Widya+Utam&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.subject=660+Teknik+kimia&rft.description=Saat+ini%2C+enzim+banyak+dimanfaatkan+untuk+berbagai+produk+komersial+karena+bersifat+selektif%2C+efisien%2C+dan+ramah+lingkungan.+Salah+satu+enzim+yang+sering+digunakan+adalah+enzim+lipase+yang+berasal+dari+bakteri.+Lipase+memiliki+kemampuan+untuk+mengkatalisis+beberapa+reaksi+sintesis+seperti+hidrolisis%2C+esterifikasi%2C+dan+transesterifikasi.+Pada+penelitian+ini+dilakukan+untuk+mempelajari+lipase+yang+dihasilkan+dari+Klebsiella+sp.+mengenai+kemampuannya+sebagai+katalis+pada+reaksi+transesterifikasi+untuk+produksi+biodiesel.+Penelitian+ini+dilakukan+bertahap%2C+yaitu+dengan+menentukan+kondisi+optimum+pertumbuhan+bakteri%2C+produksi+enzim%2C+pemurnian+ekstrak+kasar+enzim%2C+penentuan+karakteristik+enzim+hasil+pemurnian%2C+dan+penggunaan+untuk+produksi+biodiesel.+Pada+tahap+penentuan+kondisi+optimum+pertumbuhan+isolat+Klebsiella+sp.+didapatkan+waktu+inkubasi+optimum+pada+jam+ke-48+pada+media+minyak+zaitun+dengan+pH+optimum+pH+7.+Produksi+enzim+menghasilkan+ekstrak+kasar+dengan+aktivitas+spesifik+2%2C52+U%2Fmg.+Ekstrak+kasar+yang+dimurnikan+secara+bertahap+menghasilkan+enzim+murni+dengan+tingkat+kemurnian+sebesar+12%2C54+kali.+Aktivitas+optimum+dari+enzim+lipase+murni+berada+pada+pH+7%2C+suhu+80%E2%84%83%2C+dan+waktu+inkubasi+selama+25+menit.+Produksi+biodiesel+menggunakan+katalis+enzim+lipase+dari+Klebsiella+sp.+pada+reaksi+transesterifikasi+minyak+kelapa+dan+metanol+yaitu+dengan+variasi+rasio+molar+1%3A3%2C+1%3A4%2C+dan+1%3A5+memberikan+nilai+uji+densitas+dan+viskositas+kinematik+yang+relatif+baik+pada+perbandingan+1%3A3+dengan+nilai+sebesar+876+kg%2Fm3+dan%0D%0A2%2C403+%C3%97+10-6+m2%2Fs.+Hasil+analisis+GC-MS+dari+reaksi+transesterifikasi+menunjukkan+jumlah+relatif+metil+ester+tertinggi+pada+rasio+molar+1%3A4.%0D%0A%0D%0AKata+kunci%3A+enzim+lipase%2C+Klebsiella+sp.%2C+transesterifikasi%2C+biodiesel%2C+katalis%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0AABSTRACT%0D%0A%0D%0ACurrently%2C+enzymes+are+widely+used+for+various+commercial+products+because+they+are+selective%2C+efficient%2C+and+environmentally+friendly.+One+of+the+enzymes+that+are+often+used+is+the+lipase+enzyme+derived+from+bacteria.+Lipase+has+the+ability+to+catalyze+several+synthesis+reactions+such+as+hydrolysis%2C+esterification%2C+and+transesterification.+This+research+was+conducted+to+study+lipase+produced+from+Klebsiella+sp.+regarding+its+ability+as+a+catalyst+in+the+transesterification+reaction+for+biodiesel+production.+This+research+was+carried+out+in+stages%2C+namely+by+determining+the+optimum+conditions+for+bacterial+growth%2C+enzyme+production%2C+purification+of+crude+enzyme+extracts%2C+determining+the+characteristics+of+the+purified+enzymes%2C+and+using+them+for+biodiesel+production.+At+the+stage+of+determining+the+optimum+conditions+for+the+growth+of+Klebsiella+sp.+The+optimum+incubation+time+was+obtained+at+48+hours+in+olive+oil+media+with+an+optimum+pH+of+pH+7.+Enzyme+production+resulted+in+a+crude+extract+with+a+specific+activity+of+2.52+U%2Fmg.+The+purified+crude+extract+gradually+produced+pure+enzymes+with+a+purity+level+of+12.54+times.+The+optimum+activity+of+pure+lipase+enzyme+is+at+pH+7%2C+temperature+80%E2%84%83%2C+and+incubation+time+is+25+minutes.+Biodiesel+production+using+the+lipase+enzyme+catalyst+from+Klebsiella+sp.+in+the+transesterification+reaction+of+coconut+oil+and+methanol%2C+namely+with+variations+in+molar+ratios+of+1%3A3%2C+1%3A4%2C+and+1%3A5+gave+relatively+good+kinematic+density+and+viscosity+test+values+at+a+ratio+of+1%3A3+to+value+of+876+kg%2Fm3+and+2.403+%C3%97+10-6+m2%2Fs.+The+results+of+the+GC-MS+analysis+of+the+transesterification+reaction+showed+the+highest+relative+amount+of+methyl+ester+at+a+molar+ratio+of+1%3A4.%0D%0A%0D%0AKeywords%3A+lipase+enzyme%2C+Klebsiella+sp.%2Ctransesterification%2C+biodiesel%2C+catalyst&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2023-03-29&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F71827%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F71827%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F71827%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++Risna+i%2C+Milenia+Widya+Utam++(2023)+PENGOPTIMALAN+KONDISI+AKTIVITAS+TRANSESTERIFIKASI+LIPASE+DARI+BAKTERI+Klebsiella+sp.+UNTUK+PRODUKSI+BIODIESEL.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F71827%2F