@misc{eprints72236,
           month = {Mei},
           title = {DETEKSI SIMULTAN BERBAGAI VIRUS YANG MENGINFEKSI 
TANAMAN CABAI RAWIT (Capsicum frutescens L.) DI KABUPATEN
PRINGSEWU DAN TANGGAMUS },
          author = {SIREGAR VIRAARRISHAPUTRI},
         address = {UNIVERSITAS LAMPUNG},
       publisher = {FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM },
            year = {2023},
             url = {http://digilib.unila.ac.id/72236/},
        abstract = {Cabai rawit (Capsicum frutescens L.) merupakan salah satu tanaman budidaya
bernilai jual tinggi yang sering digunakan sebagai bahan penambah cita rasa masakan
di Indonesia. Namun, besarnya jumlah permintaan cabai rawit menyebabkan
keberadaan tanaman ini mengalami fluktuasi. Dalam meningkatkan produksi,
budidaya tanaman ini mengalami berbagai kendala salah satunya infeksi virus.
Penelitian ini dilaksanakan dalam dua tahapan, yaitu koleksi sampel di Kabupaten
Pringsewu dan Tanggamus, serta analisis molekuler di Laboratorium Bioteknologi,
Jurusan Proteksi Tanaman, Fakultas Pertanian, Universitas Lampung pada bulan
Oktober 2022 hingga Februari 2023. Penelitian ini bertujuan untuk mendeteksi
adanya beberapa jenis virus pada cabai rawit, mengetahui karakter virus, serta
mengetahui kekerabatan isolat virus yang berbeda. Penelitian ini menggunakan teknik
Multiplex Polymerase Chain Reaction (PCR) untuk mengamplifikasi dua virus yang
diduga menginfeksi tanaman cabai rawit serta untuk mendapatkan data hasil
perunutan basa nukleotida yang dilanjutan dengan membandingkan isolat virus
dengan isolat virus di daerah lain menggunakan online software BLAST. Isolat virus
selanjutnya dianalisis dengan software MEGA 11 dan program ClustalW. Kemudian
dilakukan analisis filogenetik untuk menentukan hubungan kekerabatan isolat virus
divisualisasikan dengan software MEGA 11. Hasil deteksi menunjukkan tanaman
cabai rawit asal Kabupaten Pringsewu dan Tanggamus positif terinfeksi TYLCV dan
PepYLCV dengan pita spesifik {$\pm$}912 bp dan {$\pm$}550 bp. Karakterisasi molekuler dari
isolat, Ca1 KH (PepYLCV), Ca1 SPG (TYLCV), dan Ca3 KH (PepYLCV)
menunjukkan jumlah basa nukleotida secara berurutan sebesar 417 basa, 884 basa,
dan 457 basa. Analisis filogenetik menunjukkan isolat Ca1 SPG (TYLCV) asal
Srikaton memiliki hubungan kekerabatan dengan isolat asal Bali, sedangkan isolat
Ca1 KH (PepYLCV) asal Srikaton dan Ca3 KH (PepYLCV) asal Gisting Permai
dalam satu kelompok yang sama menunjukkan isolat mengarah pada spesiasi. 

Kata kunci: Cabai rawit (Capsicum frutescens L.), Lampung, Multiplex PCR,Polymerase Chain Reaction (PCR), Virus. 





abstract

Chili pepper (Capsicum frutescens L.) is one of the high-value cultivated plants that
is often used as an enhancer of the taste of cuisine in Indonesia. However, the large
amount of demand for cayenne pepper causes the existence of this plant to fluctuate.
In increasing production, the cultivation of this plant experiences various obstacles,
one of which is viral infection. This research was carried out in two stages, namely
sample collection in Pringsewu and Tanggamus Regencies, and molecular analysis
at the Biotechnology Laboratory, Department of Plant Protection, Faculty of
Agriculture, University of Lampung from October 2022 to February 2023. This study
aims to detect the presence of several types of viruses in chili pepper, determine the
character of the virus, and find out the kinship of different virus isolates. This study
used the Multiplex Polymerase Chain Reaction (PCR) technique to amplify two
viruses suspected of infecting chili pepper plants and to obtain data on the results of
nucleotide base traceability which was continued by comparing virus isolates with
virus isolates in other regions using online BLAST software. Virus isolates were
further analyzed with MEGA 11 software and the ClustalW program. Then
aphylogenetic analysis to determine the kinship relationships of virus isolates was
visualized with MEGA 11 software. The detection results showed chili pepper plants
from Pringsewu and Tanggamus districts were positively infected with TYLCV and
PepYLCV with specific bands of {$\pm$}912 bp and {$\pm$}550 bp. Molecular characterization of
isolates, Ca1 KH (PepYLCV), Ca1 SPG (TYLCV), and Ca3 KH (PepYLCV) shows
the number of nucleotide bases respectively of 417 bases, 884 bases, and 457 bases.
Phylogenetic analysis shows that Ca1 SPG (TYLCV) isolates from Srikaton are
related to isolates from Bali, while Ca1 KH (PepYLCV) isolates from Srikaton and
Ca3 KH (PepYLCV) from Gisting Permai in the same group show       isolates showing
speciation. 

Keywords: Chili pepper (Capsicum frutescens L.), Lampung, Multiplex PCR,Polymerase Chain Reaction (PCR), Virus. }
}