?url_ver=Z39.88-2004&rft_id=1917021003&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=DETEKSI+SIMULTAN+BERBAGAI+VIRUS+YANG+MENGINFEKSI+%0D%0ATANAMAN+CABAI+RAWIT+(Capsicum+frutescens+L.)+DI+KABUPATEN%0D%0APRINGSEWU+DAN+TANGGAMUS+&rft.creator=VIRAARRISHAPUTRI%2C+SIREGAR&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=570+Biologi&rft.description=Cabai+rawit+(Capsicum+frutescens+L.)+merupakan+salah+satu+tanaman+budidaya%0D%0Abernilai+jual+tinggi+yang+sering+digunakan+sebagai+bahan+penambah+cita+rasa+masakan%0D%0Adi+Indonesia.+Namun%2C+besarnya+jumlah+permintaan+cabai+rawit+menyebabkan%0D%0Akeberadaan+tanaman+ini+mengalami+fluktuasi.+Dalam+meningkatkan+produksi%2C%0D%0Abudidaya+tanaman+ini+mengalami+berbagai+kendala+salah+satunya+infeksi+virus.%0D%0APenelitian+ini+dilaksanakan+dalam+dua+tahapan%2C+yaitu+koleksi+sampel+di+Kabupaten%0D%0APringsewu+dan+Tanggamus%2C+serta+analisis+molekuler+di+Laboratorium+Bioteknologi%2C%0D%0AJurusan+Proteksi+Tanaman%2C+Fakultas+Pertanian%2C+Universitas+Lampung+pada+bulan%0D%0AOktober+2022+hingga+Februari+2023.+Penelitian+ini+bertujuan+untuk+mendeteksi%0D%0Aadanya+beberapa+jenis+virus+pada+cabai+rawit%2C+mengetahui+karakter+virus%2C+serta%0D%0Amengetahui+kekerabatan+isolat+virus+yang+berbeda.+Penelitian+ini+menggunakan+teknik%0D%0AMultiplex+Polymerase+Chain+Reaction+(PCR)+untuk+mengamplifikasi+dua+virus+yang%0D%0Adiduga+menginfeksi+tanaman+cabai+rawit+serta+untuk+mendapatkan+data+hasil%0D%0Aperunutan+basa+nukleotida+yang+dilanjutan+dengan+membandingkan+isolat+virus%0D%0Adengan+isolat+virus+di+daerah+lain+menggunakan+online+software+BLAST.+Isolat+virus%0D%0Aselanjutnya+dianalisis+dengan+software+MEGA+11+dan+program+ClustalW.+Kemudian%0D%0Adilakukan+analisis+filogenetik+untuk+menentukan+hubungan+kekerabatan+isolat+virus%0D%0Adivisualisasikan+dengan+software+MEGA+11.+Hasil+deteksi+menunjukkan+tanaman%0D%0Acabai+rawit+asal+Kabupaten+Pringsewu+dan+Tanggamus+positif+terinfeksi+TYLCV+dan%0D%0APepYLCV+dengan+pita+spesifik+%C2%B1912+bp+dan+%C2%B1550+bp.+Karakterisasi+molekuler+dari%0D%0Aisolat%2C+Ca1+KH+(PepYLCV)%2C+Ca1+SPG+(TYLCV)%2C+dan+Ca3+KH+(PepYLCV)%0D%0Amenunjukkan+jumlah+basa+nukleotida+secara+berurutan+sebesar+417+basa%2C+884+basa%2C%0D%0Adan+457+basa.+Analisis+filogenetik+menunjukkan+isolat+Ca1+SPG+(TYLCV)+asal%0D%0ASrikaton+memiliki+hubungan+kekerabatan+dengan+isolat+asal+Bali%2C+sedangkan+isolat%0D%0ACa1+KH+(PepYLCV)+asal+Srikaton+dan+Ca3+KH+(PepYLCV)+asal+Gisting+Permai%0D%0Adalam+satu+kelompok+yang+sama+menunjukkan+isolat+mengarah+pada+spesiasi.+%0D%0A%0D%0AKata+kunci%3A+Cabai+rawit+(Capsicum+frutescens+L.)%2C+Lampung%2C+Multiplex+PCR%2CPolymerase+Chain+Reaction+(PCR)%2C+Virus.+%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0Aabstract%0D%0A%0D%0AChili+pepper+(Capsicum+frutescens+L.)+is+one+of+the+high-value+cultivated+plants+that%0D%0Ais+often+used+as+an+enhancer+of+the+taste+of+cuisine+in+Indonesia.+However%2C+the+large%0D%0Aamount+of+demand+for+cayenne+pepper+causes+the+existence+of+this+plant+to+fluctuate.%0D%0AIn+increasing+production%2C+the+cultivation+of+this+plant+experiences+various+obstacles%2C%0D%0Aone+of+which+is+viral+infection.+This+research+was+carried+out+in+two+stages%2C+namely%0D%0Asample+collection+in+Pringsewu+and+Tanggamus+Regencies%2C+and+molecular+analysis%0D%0Aat+the+Biotechnology+Laboratory%2C+Department+of+Plant+Protection%2C+Faculty+of%0D%0AAgriculture%2C+University+of+Lampung+from+October+2022+to+February+2023.+This+study%0D%0Aaims+to+detect+the+presence+of+several+types+of+viruses+in+chili+pepper%2C+determine+the%0D%0Acharacter+of+the+virus%2C+and+find+out+the+kinship+of+different+virus+isolates.+This+study%0D%0Aused+the+Multiplex+Polymerase+Chain+Reaction+(PCR)+technique+to+amplify+two%0D%0Aviruses+suspected+of+infecting+chili+pepper+plants+and+to+obtain+data+on+the+results+of%0D%0Anucleotide+base+traceability+which+was+continued+by+comparing+virus+isolates+with%0D%0Avirus+isolates+in+other+regions+using+online+BLAST+software.+Virus+isolates+were%0D%0Afurther+analyzed+with+MEGA+11+software+and+the+ClustalW+program.+Then%0D%0Aaphylogenetic+analysis+to+determine+the+kinship+relationships+of+virus+isolates+was%0D%0Avisualized+with+MEGA+11+software.+The+detection+results+showed+chili+pepper+plants%0D%0Afrom+Pringsewu+and+Tanggamus+districts+were+positively+infected+with+TYLCV+and%0D%0APepYLCV+with+specific+bands+of+%C2%B1912+bp+and+%C2%B1550+bp.+Molecular+characterization+of%0D%0Aisolates%2C+Ca1+KH+(PepYLCV)%2C+Ca1+SPG+(TYLCV)%2C+and+Ca3+KH+(PepYLCV)+shows%0D%0Athe+number+of+nucleotide+bases+respectively+of+417+bases%2C+884+bases%2C+and+457+bases.%0D%0APhylogenetic+analysis+shows+that+Ca1+SPG+(TYLCV)+isolates+from+Srikaton+are%0D%0Arelated+to+isolates+from+Bali%2C+while+Ca1+KH+(PepYLCV)+isolates+from+Srikaton+and%0D%0ACa3+KH+(PepYLCV)+from+Gisting+Permai+in+the+same+group+show+++++++isolates+showing%0D%0Aspeciation.+%0D%0A%0D%0AKeywords%3A+Chili+pepper+(Capsicum+frutescens+L.)%2C+Lampung%2C+Multiplex+PCR%2CPolymerase+Chain+Reaction+(PCR)%2C+Virus.+&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2023-05-22&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F72236%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F72236%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F72236%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++VIRAARRISHAPUTRI%2C+SIREGAR++(2023)+DETEKSI+SIMULTAN+BERBAGAI+VIRUS+YANG+MENGINFEKSI+TANAMAN+CABAI+RAWIT+(Capsicum+frutescens+L.)+DI+KABUPATEN+PRINGSEWU+DAN+TANGGAMUS.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F72236%2F