?url_ver=Z39.88-2004&rft_id=1717011071&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=ISOLASI+DAN+KARAKTERISASI+SENYAWA+METABOLIT+SEKUNDER+MIKROBA+SEDIMEN+BAKAU+SERTA+UJI+AKTIVITAS+ANTIBAKTERI&rft.creator=ANISYA+REIKA+%2C+AGUSTINA&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=510+Matematika&rft.description=Ekosistem+bakau+menyimpan+kelimpahan+karbon+organik+dan+kandungan+nutrisi+yang+tinggi+pada+sedimennya%2C+hal+ini+menyediakan+adanya+habitat+bagi+komunitas+mikroorganisme.++Mikroorganisme+yang+berasosiasi+dengan+ekosistem+bakau+diketahui+memiliki+potensi+untuk+memproduksi+senyawa+metabolit+sekunder+yang+memiliki+bioaktivitas+sebagai+antibakteri.++Pemanfaatan+antibiotik+dalam+praktik+klinis+saat+ini+semakin+meningkat+karena+tersebarnya+patogen+resisten+antibiotik.++Penelitian+ini+bertujuan+untuk+untuk+mengetahui+adanya+senyawa+metabolit+sekunder+yang+dihasilkan+oleh+mikroba+sedimen+bakau+di+sekitar+kawasan+hutan+bakau+Lampung+serta+bioaktivitasnya+sebagai+antibakteri.%0D%0APenelitian+ini+dilaksanakan+di+UPT-LTSIT+Universitas+Lampung.++Penelitian+didahului+dengan+pengambilan+sampel+sedimen+bakau+pada+Kawasan+hutan+bakau+Petengoran+dan+Kawasan+Pantai+Dewi+Mandapa%2C+Lampung.++Isolasi+mikroorganisme+dari+sedimen+bakau+menggunakan+media+koloid+kitin+menunjukkan+bahwa+Isolat+mikroba+BL2R1-2+yang+berasal+dari+sedimen+bakau+pada+lokasi+Kawasan+hutan+bakau+Petengoran%2C+terindikasi+sebagai+Actinomycetes+genus+Streptomyces+sp.++Uji+bioaktivitas+antibakteri+menunjukkan+bahwa+ekstrak+isolat+BL2R1-2+memiliki+potensi+menghambat+pertumbuhan+Staphylococcus+aureus+dengan+nilai+penghambatan+sebesar+56%2C75%25+pada+masa+inkubasi+18+jam.++%0D%0AKarakterisasi+senyawa+yang+diperoleh+dilakukan+dengan+metode+Fourier+Transform+Infrared+Spectroscopy+(FT-IR)%2C+dan+Liquid+Chromatography+Mass+Spectroscoy-Mass+Spectroscoy+(LC-MS%2FMS)+menunjukkan+bahwa+Isolat+BL2R1-2+diduga+mampu+memproduksi+senyawa+alkaloid+diketopiperazin+dengan+nilai+ion+puncak+m%2Fz+210.1452+dengan+formula+C11H18N2O2.++Prediksi+senyawa+dikonfirmasi+dengan+analisis+FT-IR+pada+puncak+serapan+3309%2C87+cm-1%3B+2922%2C23+cm-1%3B+1654%2C83+cm-1+sebagai+gugus+N-H%2C+C-H+alifatik+dan+C%3DO.++serta+serapan+gugus+C-N+pada+bilangan+gelombang+1371%2C6+cm-1+dan+1237%2C47+cm-1.%0D%0A%0D%0AKata+kunci%3A+sedimen+bakau%2C+mikroorganisme%2C+antibakteri%2C+karakterisasi+senyawa+metabolit+sekunder%0D%0A+%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0AABSTRACT%0D%0A%0D%0AMangrove+ecosystems+have+an+abundance+of+organic+carbon+and+high+nutrient+content+in+their+sediments%2C+providing+habitat+for+communities+of+microorganisms.+Microorganisms+associated+with+mangrove+ecosystems+are+known+to+have+the+potential+to+produce+secondary+metabolite+compounds+that+have+bioactivity+as+antibacterials.++Antibiotics+in+clinical+practice+are+becoming+increasingly+due+to+the+spread+of+antibiotic-resistant+pathogens.++This+study+aims+to+determine+the+presence+of+secondary+metabolite+compounds+produced+by+mangrove+sediment+microbes+around+the+Lampung+mangrove+forest+area+and+their+bioactivity+as+antibacterial.+This+research+was+accomplished+at+UPT-LTSIT%2C+University+of+Lampung.++The+study+was+preceded+by+mangrove+sediment+sampling+in+the+Petengoran+mangrove+forest+Area+and+Dewi+Mandapa+Beach+Area%2C+Lampung.++Isolation+of+microorganisms+from+mangrove+sediments+using+chitin+colloidal+media+showed+that+BL2R1-2+microbial+isolates+from+mangrove+sediments+at+the+Petengoran+mangrove+forest+Area+indicated+as+Actinomycetes+genus+Streptomyces+sp.+Antibacterial+bioactivity+test+showed+that+BL2R1-2+isolate+extract+has+the+potential+to+inhibit+the+growth+of+Staphylococcus+aureus+with+an+inhibition+value+of+56.75%25+during+an+18-hour+incubation+period.++The+characterization+of+the+compounds+obtained+was+carried+out+using+the+Fourier+Transform+Infrared+Spectroscopy+(FT-IR)+method%2C+and+Liquid+Chromatography+Mass+Spectroscoy-Mass+Spectroscopy+(LC-MS%2FMS)+showed+that+isolate+BL2R1-2+was+thought+to+be+capable+of+producing+diketopiperazine+alkaloid+compounds+at+the+peak+ion+m%2Fz+210.1452+with+the+formula+C11H18N2O2.+The+compound+prediction+was+confirmed+by+FT-IR+analysis+at+the+absorption+of++3309.87+cm-1%3B+2922.23+cm-1%3B+1654.83+cm-1+as+aliphatic+N-H%2C+C-H%2C+and+C%3DO+groups+also+absorption+of+C-N+group+at+wave+numbers+1371.6+cm-1+and+1237.47+cm-1.%0D%0A%0D%0AKata+kunci%3A+mangrove+sediment%2C+microorganisms%2C+antibacteri%2C+characterization+of+secondary+metabolite+compound&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2023-05-31&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F72272%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F72272%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F72272%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++ANISYA+REIKA+%2C+AGUSTINA++(2023)+ISOLASI+DAN+KARAKTERISASI+SENYAWA+METABOLIT+SEKUNDER+MIKROBA+SEDIMEN+BAKAU+SERTA+UJI+AKTIVITAS+ANTIBAKTERI.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F72272%2F