%A 2127021009 Mai Sari
%T KARAKTERISASI BIOLOGI DAN EFEKTIVITAS PROTEKSI
SILANG STRAIN LEMAH TERHADAP SUPER INFEKSI STRAIN
GANAS Pepper yellow leaf curl virus (PepYLCV) PADA
CABAI RAWIT (Capsicum frutescens L.)
%X ABSTRAK
KARAKTERISASI BIOLOGI DAN EFEKTIVITAS PROTEKSI
SILANG STRAIN LEMAH TERHADAP SUPER INFEKSI
STRAIN GANAS Pepper yellow leat curl virus (PepYLCV) PADA
CABAI RAWIT (Capsicum frutescens L.)

Cabai rawit (Capsicum frutescens L.) merupakan salah satu tanaman
hortikultura yang memiliki nilai ekonomi cukup tinggi. Cabai rawit banyak
digunakan sebagai bumbu dan penambah cita rasa makanan karena rasa pedas yang
berasal dari kandungan capsaicin. Salah satu virus yang menginfeksi cabai rawit
yaitu Pepper yellow leaf curl virus (PepYLCV). Infeksi PepYLCV dapat
menyebabkan kegagalan panen serta menimbulkan kerugian besar pada petani
cabai rawit. Salah satu alternatif pengendalian yaitu dengan proteksi silang
menggunakan strain lemah. Penelitian ini bertujuan untuk mendapatkan strain
lemah PepYLCV yang menginfeksi populasi alami pada cabai rawit, mengetahui
karakter biologi dan molekuler PepYLCV pada cabai rawit, dan mengetahui
efektivitas proteksi silang strain lemah PepYLCV terhadap super infeksi strain
ganas pada cabai rawit. Hipotesis yang diajukan dalam penelitian adalah infeksi
proteksi silang yang berasosiasi dengan strain lemah PepYLCV dapat memproteksi
tanaman cabai rawit dari super infeksi strain ganas PepYLCV. Penelitian ini
dilaksanakan di Labolatorium Bioteknologi, Fakultas Pertanian, dan Labolatorium
Botani Fakultas FMIPA Universitas Lampung, bulan Juli 2022 - Februari 2023.
Penelitian ini menggunakan 3 tahapan yaitu inokulasi PepYLCV, analisis
karakterisasi molekuler dan morfologi, serta analisis fisiologi. Data diolah dengan
ANOVA dan dilanjutkan dengan uji BNJ pada taraf nyata 5%. Berdasarkan hasil
penelitian diperoleh isolat C1 asal Desa Dadapan sebagai isolat lemah PepYLCV
dan isolat C2 asal Desa Srikaton sebagai isolat ganas PepYLCV. Hasil isolat strain
lemah menginfeksi populasi alami menunjukkan sampel terinfeksi PepYLCV
berdasarkan hasil amplifikasi pita DNA spesifik. Hasil karakter biologi
menunjukkan strain lemah asal Dadapan dan strain ganas asal Srikaton berdasarkan
intensitas gejala, sedangkan karakter molekuler strain lemah dan ganas berbeda
berdasarkan jarak genetik. Serta strain lemah mampu menekan laju infeksi strain
ganas PepYLCV, berdasarkan variasi gejala, keparahan penyakit, ketahanan
tanaman, kandungan klorofil, dan karbohidrat.
Kata kunci: Biologi, cabai rawit (Capsicum frutescens L.), Pepper yellow leaf curl
virus (PepYLCV), proteksi silang

Cayenne pepper (Capsicum frutescens L.) is a horticultural crop that has high
economic value. Cayenne pepper is widely used as a spice and food flavor enhancer
because of the spicy taste that comes from the capsaicin content. One of the viruses
that infect cayenne pepper is Pepper yellow leaf curl virus (PepYLCV). Infection
PepYLCV can cause crop failure and cause big losses to farmers Cayenne pepper.
One control alternative is cross protection using weak strains. This study aims to
obtain weak strains PepYLCV that infect natural populations Cayenne pepper.,
know the biological and molecular characters PepYLCV on Cayenne pepper, and
determine the effectiveness of weak strain cross protection PepYLCV against
superinfection of malignant strains in cayenne pepper. The hypothesis proposed in
this study is cross-protection infection associated with weak strains PepYLCV can
protect cayenne pepper plants from super-infection of malignant strains PepYLCV.
This research was conducted in Laboratory Bioteknologi, Fakultas Pertanian, and
Laboratory Botani Fakultas FMIPA Universitas Lampung, month July 2022 -
February 2023. This study used 3 stages, namely PepYLCV inoculation, molecular
and morphological characterization analysis, and physiological analysis. The data
was processed using ANOVA and continued with the BNJ test at the real level 5%.
Based on the research results, it was found that C1 isolate from the village Dadapan
as a weak isolate PepYLCV and isolate C2 village origin Srikaton as a malignant
isolate PepYLCV. The results of isolates of weak strains infecting natural
populations indicate infected samples PepYLCV based on the amplification of
specific DNA bands. The results of the biological characters showed a weak strain
of origin Dadapan and malignant strains of origin Srikaton based on the intensity
of symptoms, while the molecular characters of weak and malignant strains differ
based on genetic distance. As well as weak strains capable of suppressing the rate
of infection of malignant strains PepYLCV, based on a variety of symptoms,
disease severity, plant resistance, chlorophyll and carbohydrate content.
Keywords: Biology, cayenne pepper (Capsicum frutescens L.), Pepper yellow leaf
curl virus (PepYLCV), cross protection
%D 2023
%I UNIVERSITAS LAMPUNG
%L eprints72912