?url_ver=Z39.88-2004&rft_id=1917021016&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=ANALISIS+KELIMPAHAN+BAKTERI+BERBASIS+DNA+PADA+GASTROINTESTINAL+TRACT+IKAN+GABUS+(Channa+striata)+BUDIDAYA+DAN+ALAM+DI+SUNGAI+TANGGAMUS&rft.creator=MUHAMMAD+ADE+%2C+NUGRAHA&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=570+Biologi&rft.description=Ikan+gabus+(Channa+striata)+merupakan+ikan+konsumsi+yang+diminati+oleh+masyarakat.+Selain+itu%2C+ikan+gabus+memiliki+kandungan+albumin+yang+tinggi+sehingga+dibutuhkan+bagi+penderita+hipoalbumin+dan+luka.+Tingginya+peminatan+ikan+gabus+membuat+masyarakat+melakukan+kegiatan+budidaya+ikan+gabus+untuk+mempercepat+pertumbuhan+dan+memenuhi+kebutuhan+masyarakat.+Ikan+gabus+adalah+jenis+ikan+predator+yang+mempunyai+penyebaran+luas+yang+secara+alami+dapat+hidup+di+danau%2C+sawah%2C+rawa%2C+tambak%2C+dan+sungai%2C+salah+satunya+sungai+yang+ada+di+daerah+Tanggamus.+Faktor+yang+membantu+pertumbuhan+ikan+gabus+yaitu+jenis+makanan+dan+bakteri+yang+terdapat+pada+gastrointestinal+tract+ikan.+Jenis+makanan+dapat+memberikan+perbedaan+kelimpahan+bakteri+pada+gastrointestinal+tract+ikan.+Penelitian+ini+bertujuan+untuk+mengetahui+perbedaan+kelimpahan+bakteri+yang+terdapat+pada+gastrointestinal+tract+ikan+gabus+alam+dan+budidaya+berbasis+PCR.+Penelitian+ini+menggunakan+metode+berbasis+analisis+DNA+untuk+melihat+kelimpahan+bakteri+yang+terdapat+pada+gastrointestinal+tract+ikan+gabus.+Penelitian+ini+memiliki+beberapa+tahapan+proses+yaitu%2C+ekstraksi+DNA+Bakteri%2C+analisis+konsentrasi+dan+kemurnian+DNA%2C+PCR%2C+dan+visualisasi+DNA+dengan+elektroforesis.+Sampel+ikan+gabus+alam+pada+penelitian+ini+berasal+dari+sungai+Tanggamus+sedangkan+ikan+gabus+budidaya+pada+penelitian+ini+berasal+dari+Budi+Fish+Farm+di+daerah+Pringsewu.+Masing+%E2%80%93+masing+sampel+diambil+dari+ikan+dengan+bobot+total+700+Gram.+Perbedaan+kelimpahan+bakteri+total+antara+ikan+gabus+budidaya+dan+alam+dianalisis+melalui+elektroforesis+hasil+PCR.+Melalui+penelitian+ini+diketahui+bahwa+bakteri+pada+gastrointestinal+tract+ikan+gabus+budidaya+maupun+alam+memiliki+kelimpahan+bakteri+Gram+positif+lebih+banyak+dibandingkan+bakteri+Gram+negatif.+Kelimpahan+bakteri+Gram+positif+pada+ikan+gabus+budidaya+lebih+banyak+dibandingkan+dengan+ikan+gabus+alam+(41+%3A+25)%2C+sedangkan+kelimpahan+bakteri+Gram+negatif+lebih+banyak+pada+ikan+gabus+alam+dibandingkan+ikan+gabus+budidaya+(23+%3A+24).%0D%0AKata+kunci%3A+Ikan+gabus+(Channa+striata)%2C+Gastrointestinal+tract%2C+Ekstraksi+DNA%2C+Nanofotometer%2C+Polymerase+Chain+Reaction%2C+Elektroforesis.%0D%0ASnakehead+fish+(Channa+striata)+a+consumption+fish+that+in+demand+by+the+public.+In+addition%2C+snakehead+fish+has+a+high+albumin+content+so+it+is+needed+for+people+with+hypoalbumin+and+wounds.+The+high+interest+in+snakehead+fish+makes+people+carry+out+snakehead+fish+farming+activities+to+accelerate+growth+and+meet+the+needs+of+the+community.+Snakehead+fish+is+a+type+of+predatory+fish+that+has+a+wide+distribution+that+can+naturally+live+in+lakes%2C+rice+fields%2C+swamps%2C+ponds%2C+and+rivers%2C+one+of+which+is+the+Tanggamus+river.+Factors+that+help+the+growth+of+snakehead+fish+are+the+types+of+food+and+bacteria+found+in+the+gastrointestinal+tract+of+fish.+The+type+of+food+can+provide+differences+in+the+abundance+of+bacteria+in+the+gastrointestinal+tract+of+fish.+This+study+aims+to+determine+the+difference+in+the+abundance+of+bacteria+found+in+the+gastrointestinal+tract+of+natural+snakehead+fish+and+PCR-based+cultivation.+This+study+used+a+method+based+on+DNA+analysis+to+see+the+abundance+of+bacteria+found+in+the+gastrointestinal+tract+of+snakehead+fish.+This+research+has+several+stages+of+the+process%2C+namely%2C+bacterial+DNA+extraction%2C+DNA+concentration+and+purity+analysis%2C+PCR%2C+and+DNA+visualization+by+electrophoresis.+Natural+snakehead+fish+samples+in+this+study+came+from+the+Tanggamus+river+while+farmed+snakehead+fish+in+this+study+came+from+Budi+Fish+Farm+in+the+Pringsewu+area.+Each+sample+was+taken+from+fish+with+a+total+weight+of+700+grams.+The+difference+in+total+bacterial+abundance+between+farmed+and+natural+snakehead+fish+was+analyzed+through+electrophoresis+of+PCR+results.+Through+this+study%2C+it+is+known+that+bacteria+in+the+gastrointestinal+tract+of+farmed+and+natural+snakehead+fish+have+an+abundance+of+more+Gram+positive+bacteria+than+Gram+negative+bacteria.+The+abundance+of+Gram+positive+bacteria+in+farmed+snakehead+fish+is+more+than+in+natural+snakehead+fish+(41%3A+25)%2C+while+the+abundance+of+Gram+negative+bacteria+is+more+in+natural+snakehead+fish+than+in+farmed+snakehead+fish+(23%3A+24).%0D%0AKeywords%3A+Snakehead+fish+(Channa+striata)%2C+Gastrointestinal+tract%2C+DNA+extraction%2C+Nanophotometer%2C+Polymerase+Chain+Reaction%2C+Electrophoresis.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2023-06-16&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F73593%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F73593%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F73593%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++MUHAMMAD+ADE+%2C+NUGRAHA++(2023)+ANALISIS+KELIMPAHAN+BAKTERI+BERBASIS+DNA+PADA+GASTROINTESTINAL+TRACT+IKAN+GABUS+(Channa+striata)+BUDIDAYA+DAN+ALAM+DI+SUNGAI+TANGGAMUS.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F73593%2F