%0 Generic
%A WIDURI , NAYUNDA SAFITRI 
%C UNIVERSITAS LAMPUNG
%D 2023
%F eprints:73718
%I FAKULTAS PERTANIAN
%T STUDI RESISTANSI Aeromonas hydrophila (Holt et al., 1994) PADA TIGA  JENIS ANTIBIOTIK DARI LOKASI AKUAKULTUR DI WILAYAH  PROVINSI BANTEN
%U http://digilib.unila.ac.id/73718/
%X   Motile aeromonas septicaemia (MAS) is a disease that has the potential to become  an epidemic in freshwater cultured fish and is caused by the bacterium Aeromonas  hydrophila. The use of antibiotics as a treatment is considered the most  effective countermeasures. The use of antibiotics that are not as recommended can  cause bacteria to become resistant. The purpose of this study was to evaluate the  resistance of A. hydrophila bacteria to three antibiotics namie oxytetracycline, tetracycline,  and enrofloxacin. The research was conducted by explorative method.  A. hydrophila was isolated from samples of pond water, inlet water, outlet water,  pond sediment, catfish (Clarias sp.), and tilapia (Oreochromis niloticus). The  samples were taken from fish farm in Banten Province. A. hydrophila isolates  were identified by biochemical tests using vitek 2compact, then A. hydrophila  susceptibility test to three antibiotics was carried out in vitro using the disc diffusion  method. A. hydrophila which was known to be resistant to certain antibiotics  was continued with the minimum inhibitory concentration (MIC) test. The results  of this study, 31 A. hydrophila isolates were obtained, where the percentage of  oxytetracycline was 6.4%, tetracycline was 3.2% and enrofloxacin did not experience  resistance. The MIC value of tetracycline and oxytetracycline in the  A. hydrophila isolates was 16 μg/ml.  Keywords : Aeromonas hydrophila, antibiotics, oxytetracycline, resistance,  tetracyclines.    Motile aeromonas septicaemia (MAS) merupakan penyakit yang berpotensi menjadi  wabah pada ikan budi daya air tawar dan disebabkan oleh bakteri Aeromonas  hydrophila. Penggunaan antibiotik sebagai pengobatan dianggap sebagai penanggulangan  yang paling efektif. Penggunaan antibiotik yang tidak sesuai anjuran dapat  mengakibatkan bakteri menjadi resistan. Tujuan dari penelitian ini untuk  mengevaluasi resistansi bakteri A. hydrophila terhadap tiga antibiotik, yaitu oksitetrasiklin,  tetrasiklin, dan enrofloksasin. Penelitian dilakukan dengan metode eksploratif.  A. hydrophila diisolasi dari sampel air kolam, air inlet, air outlet, sedimen  kolam, lele (Clarias sp.), dan nila (Oreochromis niloticus). Sampel tersebut diambil  dari pembudi daya ikan di Provinsi Banten. Isolat A. hydrophila diidentifikasi  dengan uji biokimia menggunakan vitek 2compact, kemudian uji kepekaan A.  hydrophila terhadap tiga antibiotik dilakukan secara in vitro menggunakan metode  difusi cakram. A. hydrophila yang diketahui resistan terhadap antibiotik tertentu  dilanjutkan dengan uji minimum inhibitory concentration (MIC). Hasil penelitian  menghasilkan 31 isolat A. hydrophila, persentase oksitetrasiklin sebesar  6,4%, tetrasiklin sebesar 3,2% dan enrofloksasin tidak ada yang resistan. Nilai  MIC dari tetrasiklin dan oksitetrasiklin pada isolat A. hydrophila sebesar 16  μg/ml.  Kata kunci : Aeromonas hydrophila, antibiotik, oksitetrasiklin, resistansi,  tetrasiklin.