?url_ver=Z39.88-2004&rft_id=1717011055&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=KARAKTERISASI+SENYAWA+EKSTRAK+ETANOL+BIJI+KOPI+ROBUSTA+(Coffea+canephora)&rft.creator=Muhlis+%2C+Setiawan&rft.subject=000+Ilmu+komputer%2C+informasi+dan+pekerjaan+umum&rft.description=Kopi+robusta+mengandung+senyawa+polifenol+yang+tinggi+dan+berfungsi+sebagai+antioksidan.+Menurut+Almoosawi+et+al.%2C+2016.+Ekstrak+etanol+senyawa+biji+kopi+robusta+mampu+menangkap+radikal+bebas%2C+namun+kandungan+senyawa+di+dalam+biji+kopi+berbeda+beda+tergantung+daerah+tempat+tumbuhnya.+Sehubungan+dengan+hal+tersebut+maka+dilakukan+penelitian+yang+bertujuan+untuk+mengkarakterisasi+ekstrak+etanol+kopi+robusta+menggunakan+metode+LC-MS%2FMS+(Liquid+Chromatograph-mass+spectrometry)+dan+menguji+aktivitas+antioksidan+pada+ekstrak+etanol+kopi+robusta+menggunakan+pereaksi+DPPH+(2%2C2-difenil-1-pikrilhidrazil).+Prosedur+penelitian+meliputi+preprasi+sampel+menggunakan+biji+kopi+robusta+giling+yang+diekstrasi+menggunakan+metode+maserasi+dengan+pelarut+etanol+200+mL+selama+2%C3%9724+jam+didapatkan+ekstrak+berwarna+hijau+kekuningan+(MS-01).+MS-01+kemudian+dipekatkan+menggunakan+rotary+evaporator.+Uji+KLT+pada+variasi+eluen+n-heksan%3A+isopropanol+(IPA)+3%3A7+dibandingkan+dengan+standar+asam+klorogenat%2C+Hasil+MS-01+menunjukkan+spot+noda+yang+sama+dengan+standar+asam+klorogenat.+MS-01+kemudian+dilakukan+pemisahan+menggunakan+MPLC+dengan+hasil+fraksi+MS-23%2C+MS-34A%2C+dan+MS-34B.+Pada+fraksi+MS-34A+diketahui+sudah+mendekati+spot+tunggal+sehingga+dilakukan+uji+LC-MS%2FMS.+Berdasarkan+hasil+interpretasi+data+LC-MS%2FMS+pada+fraksi+MS-34A+memiliki+struktur+dominan+asam+klorogenat.+MS-34A+dilakukan+Uji+lanjutan+yaitu+uji+aktivitas+antioksidan+menggunakan+pereaksi+DPPH+(2%2C2-difenil-1-pikrilhidrazil).+Hasil+menunjukkan+perubahan+warna+pada+pereaksi+DPPH+dari+ungu+menjadi+kuning+pudar+sehingga+MS-34A+memiliki+aktivitas+antioksidan.%0D%0A%0D%0AKata+kunci%3A+Kopi+Robusta+(Coffea+canephora)%2C+LC-MS%2FMS%2C+DPPH%2C+MS%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0AABSTRACT%0D%0A%0D%0ARobusta+coffee+contains+high+polyphenolic+compounds+which+function+as+antioxidants.+According+to+Almoosawi+et+al.%2C+2016.+The+ethanol+extract+of+robusta+coffee+bean+compounds+is+able+to+scavenge+free+radicals%2C+but+the+compound+content+in+coffee+beans+varies+depending+on+the+region+where+it+grows.+In+this+regard%2C+a+study+was+conducted+which+aimed+to+characterize+the+ethanol+extract+of+robusta+coffee+using+the+LC-MS%2FMS+(Liquid+Chromatograph-mass+spectrometry)+method+and+to+test+the+antioxidant+activity+of+the+ethanol+extract+of+robusta+coffee+using+DPPH+(2%2C2-diphenyl-1-picrylhydrazyl).+The+research+procedure+included+sample+preparation+using+ground+robusta+coffee+beans+which+were+extracted+by+maceration+method+with+200+mL+ethanol+solvent+for+2%C3%9724+hours+to+obtain+a+yellowish+green+extract+(MS-01).+MS-01+was+then+concentrated+using+a+rotary+evaporator.+The+TLC+test+on+the+eluent+variation+n-hexane%3Aisopropanol+(IPA)+3%3A7+compared+to+the+chlorogenic+acid+standard%2C+the+MS-01+results+showed+the+same+stain+spots+as+the+chlorogenic+acid+standard.+MS-01+was+then+separated+using+MPLC+with+the+results+of+fractions+MS-23%2C+MS-34A%2C+and+MS-34B.+In+the+MS-34A+fraction+it+is+known+that+it+is+close+to+a+single+point%2C+so+the+LC-MS%2FMS+test+is+carried+out.+Based+on+the+interpretation+of+LC-MS%2FMS+data%2C+the+MS-34A+fraction+has+a+dominant+structure+of+chlorogenic+acid.+MS-34A+was+carried+out+as+a+follow-up+test%2C+namely+antioxidant+activity+test+using+DPPH+reagent+(2%2C2-diphenyl-1-picrylhidrazyl).+The+results+showed+that+there+was+a+color+change+in+the+DPPH+reagent+from+purple+to+faded+yellow+so+that+MS-34A+had+antioxidant+activity.%0D%0A%0D%0AKeywords%3A+Robusta+Coffee+(Coffea+canephora)%2C+LC-MS%2FMS%2C+DPPH%2C+MS&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2023-06-20&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F73897%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F73897%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F73897%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++Muhlis+%2C+Setiawan++(2023)+KARAKTERISASI+SENYAWA+EKSTRAK+ETANOL+BIJI+KOPI+ROBUSTA+(Coffea+canephora).++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F73897%2F