%0 Thesis
%9 Masters
%A DIAN NELI , PRATIWI
%B PROGRAM STUDI MAGISTER ILMU LINGKUNGAN PASCASARJANA 
%D 2023
%F eprints:74205
%I UNIVERSITAS LAMPUNG
%T APLIKASI ANALISIS MOLEKULER DNA MITOKONDRIA  CYTHOCROME OXYDASE SUB-UNIT 1 (COI) UNTUK KONFIRMASI  KERAGAMAN GENETIK GAJAH SUMATERA (Elephas maximus  sumatranus) BERBASIS KOTORAN DI SUGIHAN SIMPANG HERAN
%U http://digilib.unila.ac.id/74205/
%X Gajah sumatera merupakan mamalia besar dengan status kritis. Populasi gajah  sumatera berada dalam 36 kantong habitat, salah satunya Sumatera Selatan  (Sumsel) yang mengalami tekanan seperti kerusakan dan fragmentasi habitat,  perburuan liar dan konflik dengan masyarakat. Perubahan habitat mengakibatkan  masuknya gajah sumatera ke perkebunan kelapa sawit. Upaya konservasi yang  dapat dilakukan adalah pengumpulan informasi keragaman genetik secara non  invasif sampling, melalui kotoran dengan analisis molekuler gen penanda  cytochrome oxidase subunit I (COI). Penelitian ini dilaksanakan pada Desember  2022-Februari 2023 di bawah program kegiatan Tropical Forest Conservasion  Action for Sumatera yang dilakukan oleh Pusat Kajian Sains Terapan Universitas  Sriwijaya. Tahapan penelitian yaitu pengambilan sampel, ekstraksi DNA,  amplifikasi, elektroforesis, sekuensing dan analisis data. Sampel kotoran gajah  sumatera sebanyak 25 sampel ditemukan pada enam blok area perkebunan sawit  plasma nutfah inti PT. Bumi Khatulistiwa Mandiri (BKM). Analisis pohon  filogenetik dari 18 sampel membentuk satu sluster yang sama mengindikasikan  memiliki kekerabatan yang dekat (homologi 83%-98%). Sub populasi gajah  sumatera di PT. BKM, memiliki keragaman genetik cukup baik dengan nilai variasi  genetik berdasarkan keragaman nukleotida dalam kategori sedang (Pi:0,67 dan  Hd:0,9485). Upaya konservasi yang dapat dilakukan yaitu pembuatan koridor,  kajian genetik lanjut serta pemetaan jalur aktif gajah sumatera.    Kata Kunci: analisis molekuler DNA, filogenetik, gajah sumatera, non invasif,  perubahan habitat        abstract    The Sumatran elephant is a large mammal with critical status. The Sumatran  elephant population resides in 36 habitat pockets, one of which is South Sumatra,  which is experiencing pressures such as habitat destruction and fragmentation,  poaching and conflict with the community. Habitat changes resulted in the entry of  Sumatran elephants into oil palm plantations. Conservation efforts that can be done  is to collect information on genetic diversity by non-invasive sampling, through  feces with molecular analysis of cytochrome oxidase subunit I (COI) marker genes.  This research was carried out in December 2022-February 2023 under the Tropical  Forest Conservation Action for Sumatra activity program conducted by the Center  for Applied Science Studies, Sriwijaya University. The stages of the research were  sampling, DNA extraction, amplification, electrophoresis, sequencing and data  analysis. Samples of 25 Sumatran elephant dung samples were found in six blocks  of the nucleus germ plasma oil palm plantation area of PT. Independent Equatorial  Earth (BKM). Phylogenetic tree analysis of the 18 samples forming the same cluster  indicates that they are closely related (homology 83%-98%). Sumatran elephant  sub-population at PT. BKM, has a fairly good genetic diversity with a value of  genetic variation based on nucleotide diversity in the moderate category (Pi: 0.67  and Hd: 0.9485). Conservation efforts that can be carried out include building  corridors, further genetic studies and mapping the active paths of the sumatran  elephant.  Keywords: DNA molecular analysis, habitat change, phylogenetic, sumatran  elephant, non-invasive,