?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=RESISTENSI+BAKTERI+Vibrio+spp.+TERHADAP+ANTIBIOTIK+OKSITETRASIKLIN%2C+ERITROMISIN%2C+DAN+CIPROFLOKSASIN&rft.creator=BERNIKA%2C+VINA+AUDIA&rft.subject=630+Pertanian+dan+teknologi+yang+berkaitan&rft.description=Vibrio+merupakan+bakteri+penyebab+penyakit+vibriosis+pada+ikan+maupun+udang.+Penggunaan+antibiotik+yang+kurang+bijak+dalam+menanggulangi+vibriosis+dapat+menyebabkan+bakteri+justru+mengalami+resistensi.+Tujuan+dari+penelitian+ini+yaitu+menganalisis+dan+mengkaji+sifat+resistensi+bakteri+Vibrio+spp.+terhadap+antibiotik+yang+sering+digunakan+dalam+kegiatan+budi+daya+perikanan.+Penelitian+ini+dilaksanakan+pada+bulan+Desember+2022+%E2%80%93+Januari+2023+yang+bertempat+di+Laboratorium+Budidaya+Perikanan%2C+Jurusan+Perikanan+dan+Kelautan%2C+Universitas+Lampung.+Dua+isolat+bakteri+Vibrio+yaitu+V.+parahaemolyticus+yang+didapatkan+dari+Balai+Veteriner+Lampung+dan+Vibrio+sp.+yang+isolasi+dari+perairan+sekitar+tambak+di+Pantai+Sebalang%2C+Lampung+Selatan.+Kedua+isolat+bakteri+Vibrio+ditumbuhkan+dalam+medium+NA+dan+diuji+sensitivitasnya+terhadap+tiga+antibiotik+oksitetrasiklin%2C+eritromisin%2C+dan+ciprofloksasin+menggunakan+kertas+cakram+dan+dilanjutkan+dengan+uji+MIC+dan+MBC+dari+ketiga+antibiotik+terhadap+kedua+isolat+Vibrio.+Uji+resistensi+bakteri+dilakukan+selama+30+hari+dengan+menumbuhkan+bakteri+pada+media+NB+yang+sudah+ditambahkan+masing-masing+antibiotik+dengan+dosis+mengacu+pada+hasil+MIC.+Sebagai+kontrol+adalah+bakteri+yang+ditumbuhkan+pada+media+NB+tanpa+penambahan+antibiotik.+Pengambilan+sampel+untuk+menghitung+kepadatan+bakteri+dilakukan+sebanyak+4+kali%2C+yaitu+pada+hari+ke-1%2C+ke-7%2C+ke-14%2C+dan+ke-30.+Kepadatan+bakteri+dihitung+menggunakan+UV+spektrofotometer+pada+panjang+gelombang+625+nm+dengan+mengacu+pada+standar+0%2C5-8+McFarland%2C+masing-masing+dengan+2+kali+ulangan.+Hasil+penelitian+yang+didapat+menunjukkan+bahwa+adanya+resistensi+bakteri+uji+terhadap+antibiotik+jenis+oksitetrasiklin%2C+eritromisin%2C+dan+ciprofloksasin+dilihat+dari+nilai+total+kepadatan+bakteri+yang+meningkat.+Bak-teri+V.+parahaemolyticus+memiliki+sifat+resistensi+tertinggi+karena+nilai+total+kepadatan+bakteri+terus+meningkat.%0D%0A%0D%0AKata+kunci%3A+V.+parahaemolyticus%2C+Vibrio+sp.%2C+resistensi%2C+antibiotik%2C+uji+in+vitro.%0D%0A%0D%0AVibrio+is+a+bacterium+that+causes+vibriosis+in+fish+and+shrimp.+The+use+of+antibiotics+that+are+not+wise+in+tackling+vibriosis+can+cause+bacteria+become+resistant.+The%0D%0Apurpose+of+this+study+was+to+analyze+and+examine+the+resistance+of+Vibrio+spp.+to%0D%0Aantibiotics+that+were+often+used+in+aquaculture.+This+research+was+carried+out+in%0D%0ADecember+2022+%E2%80%93+January+2023+at+the+Aquaculture+Laboratory%2C+Department+of%0D%0AFisheries+and+Marine+Affairs%2C+University+of+Lampung.+Two+isolates+of+Vibrio+bacteria+were+V.+parahaemolyticus+obtained+from+veterinary+center+of+Lampung+and%0D%0AVibrio+sp.+the+isolation+of+the+waters+around+the+pond+at+Sebalang+Beach%2C+South%0D%0ALampung.+The+two+isolates+of+Vibrio+bacteria+were+grown+in+NA+medium+and%0D%0Atested+for+sensitivity+to+three+antibiotics+oxytetracyclin%2C+erythromycin%2C+and+ciprofloxacin+using+paper+discs+and+followed+by+MIC+and+MBC+tests+of+the+three+antibiotics+against+both+isolates+Vibrio.+The+bacterial+resistance+test+was+conducted+for%0D%0A30+days+by+growing+bacteria+on+NB+media+that+had+been+added+to+each+antibiotic%0D%0Awith+a+dose+referring+to+the+MIC+results.+As+a+control+was+bacteria+grown+on+NB%0D%0Amedia+without+the+addition+of+antibiotics.+Sampling+to+calculate+the+density+of%0D%0Abacteria+was+carried+out+4+times+on+the+1st%2C+7th%2C+14th%2C+and+30th+days.+Bacterial+density+was+calculated+using+a+UV+spectrophotometer+at+a+wavelength+of+625+nm+with%0D%0Areference+to+the+standard+0%2C5-8+McFarland%2C+each+with+2+repetitions.+The+results%0D%0Ashowed+that+the+resistance+of+test+bacteria+to+antibiotics+such+as+oxytetracycline%2C%0D%0Aerythromycin%2C+and+ciprofloxacin+seen+from+the+value+of+the+total+bacterial+density%0D%0Aincreased.+Bacteria+V.+parahaemolyticus+had+the+highest+resistance+because+the%0D%0Avalue+of+the+total+bacterial+density+is+constantly+increasing.%0D%0AKeywords%3A+V.+parahaemolyticus%2C+Vibrio+sp.%2C+resistance%2C+antibiotics%2C+in+vitro%0D%0Aassays&rft.publisher=FAKULTAS+PERTANIAN&rft.date=2023-06-26&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F75427%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F75427%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F75427%2F3%2FSKRIPSI%2520TANPA%2520PEMBAHASAN.pdf&rft.identifier=++BERNIKA%2C+VINA+AUDIA++(2023)+RESISTENSI+BAKTERI+Vibrio+spp.+TERHADAP+ANTIBIOTIK+OKSITETRASIKLIN%2C+ERITROMISIN%2C+DAN+CIPROFLOKSASIN.++FAKULTAS+PERTANIAN%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F75427%2F