?url_ver=Z39.88-2004&rft_id=1917021045&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=STUDI+MORFOLOGI+DAN+KARAKTER+KRISTAL+PROTEIN+ISOLAT%0D%0ABacillus+thuringiensis&rft.creator=RACHMAT%2C+NUGRAHA+INDRA+&rft.subject=001+Ilmu+pengetahuan&rft.description=Bacillus+merupakan+bakteri+Gram+positif+berbentuk+batang+dan+sering+dimanfaatkan%0D%0Adalam+bidang+bioteknologi.+Bakteri+Bacillus+thuringiensis+(Bt)+memiliki%0D%0Akarakteristik+yang+berbeda+dengan+spesies+Bacillus+lain%2C+yaitu+dapat+menghasilkan%0D%0Akristal+protein+yang+bersifat+toksik+bagi+serangga+pada+fase+sporulasi.+Kristal%0D%0Ainsektisida+tersebut+terdiri+dari+satu+atau+lebih+protein+kristal+(Cry)+dan+sitolitik%0D%0A(Cyt)%2C+yang+dikenal+sebagai+%CE%B4-endotoksin.+Spesifisitas+dan+aktivitas+Bacillus%0D%0Athuringiensis+terhadap+inangnya+dikaitkan+dengan+jenis+dan+bentuk+%CE%B4-endotoksin%0D%0Ayang+dihasilkan.+Penelitian+ini+dilakukan+untuk+mengetahui+awal+terbentuknya%0D%0Akristal+protein+pada+waktu+pertumbuhan+bakteri+serta+karakter+bentuk+kristal+protein%0D%0Adari+enam+isolat+Bacillus+thuringiensis+yang+diisolasi+dari+tanah+Kebun+Raya+Liwa%0D%0A(KRL)+Lampung.+Metode+penelitian+yang+digunakan+adalah+deskriptif+kualitatif%0D%0Ayang+dipresentasikan+dalam+bentuk+gambar+dan+tabel.+Identifikasi+morfologi+kristal%0D%0Aprotein+bakteri+Bacillus+thuringiensis+selama+fase+sporulasi+dilakukan+dengan%0D%0Amenggunakan+Scanning+Electron+Microscope+(SEM).+Sedangkan+pengamatan%0D%0Awaktu+terbentuknya+kristal+dilakukan+dengan+metode+pengecatan+menggunakan%0D%0AComassie+Brilliant+Blue+dan+Crystal+Violet+dengan+interval+waktu+setiap+tiga+jam%0D%0Asekali+selama+48+jam.+Gambar+hasil+SEM+menunjukkan+bahwa+setiap+isolat%0D%0Amemiliki+lebih+dari+satu+bentuk+kristal+protein+dengan+tujuh+variasi+morfologi+kristal%0D%0Aprotein%2C+yaitu+ovoid+(oval)%2C+spherical+(bulat)%2C+bipiramidal+segitiga%2C+rectangle+(empat%0D%0Apersegi+panjang)%2C+elongate+(memanjang)%2C+cuboid+(kotak)%2C+serta+geometrical.%0D%0ATerbentuknya+kristal+protein+pada+keenam+isolat+menunjukkan+waktu+yang+berbeda.%0D%0ASecara+keseluruhan+kristal+protein+sudah+terlihat+pada+jam+pertumbuhan+ke-3+hingga%0D%0Ake-18.+Waktu+terbentuknya+kristal+tersebut+dapat+dijadikan+sebagai+referensi+untuk%0D%0Apengujian+aktivitas+toksisitas+isolat.%0D%0AKata+kunci+%3A+Bacillus+thuringiensis%2C+kristal+protein%2C+morfologi%2C+Scanning+Electron%0D%0AMicroscope%2C+waktu+terbentuknya+kristal%0D%0A%0D%0ABacillus+is+a+Gram-positive+rod-shaped+bacterium+and+is+often+used+in+the+field+of%0D%0Abiotechnology.+Bacillus+thuringiensis+(Bt)+bacteria+can+produce+protein+crystals+that%0D%0Aare+toxic+to+insects+during+the+sporulation+phase.+The+insecticide+crystals+consist+of%0D%0Aone+or+more+crystalline+(Cry)+and+cytolytic+(Cyt)+proteins%2C+which+are+known+as+%CE%B4-%0D%0Aendotoxins.+The+specificity+and+activity+of+Bacillus+thuringiensis+on+the+host+are%0D%0Arelated+to+the+type+and+form+of+the+%CE%B4-endotoxin+produced.+This+research+was%0D%0Aconducted+to+determine+the+initial+formation+of+protein+crystals+during+bacterial%0D%0Agrowth+and+the+character+of+the+protein+crystal+shape+of+six+isolates+of+Bacillus%0D%0Athuringiensis+isolated+from+the+soil+of+the+Liwa+Botanical+Garden+Lampung.+The%0D%0Aresearch+method+used+is+descriptive+qualitative+which+is+presented+in+the+form+of%0D%0Apictures+and+tables.+Identification+of+the+protein+crystal+morphology+of+Bacillus%0D%0Athuringiensis+was+carried+out+using+a+Scanning+Electron+Microscope+(SEM).+While%0D%0Aobserving+the+time+of+crystal+formation+was+carried+out+by+staining+method+using%0D%0AComassie+Brilliant+Blue+and+Crystal+Violet+with+intervals+every+three+hours+for+48%0D%0Ahours.+SEM+results+show+that+each+isolate+has+more+than+one+protein+crystal+shape%0D%0Awith+seven+variations+of+protein+crystal+morphology%2C+namely+ovoid%2C+spherical%2C%0D%0Atriangular+bipyramidal%2C+rectangular%2C+elongate%2C+cuboid%2C+as+well+as+geometrical.+The%0D%0Aformation+of+protein+crystals+in+the+six+isolates+showed+different+times.+The+time+of%0D%0Aprotein+crystals+formation+were+seen+at+the+3rd+to+18th+hour+of+growth.+These+results%0D%0Acan+be+used+as+a+reference+for+testing+the+toxicity+activity+of+isolates.%0D%0AKeywords+%3A+Bacillus+thuringiensis%2C+morphology%2C+protein+crystal%2C+Scanning+Electron%0D%0AMicroscope%2C+time+of+crystal+formation%0D%0A%0D%0A&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2023-07-24&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F75570%2F1%2F1.%2520ABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F75570%2F2%2F2.%2520SKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F75570%2F3%2F3.%2520SKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++RACHMAT%2C+NUGRAHA+INDRA+++(2023)+STUDI+MORFOLOGI+DAN+KARAKTER+KRISTAL+PROTEIN+ISOLAT+Bacillus+thuringiensis.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F75570%2F