?url_ver=Z39.88-2004&rft_id=2017011076&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=BIOTRANSFORMASI+KULIT+UDANG+MENJADI+CHITOOLIGOSACCHARIDE+(COS)+OLEH+FUNGI+LAUT%0D%0ADAN+UJI+BIOAKTIVITAS+SEBAGAI+ANTIMIKROBA&rft.creator=Muhammad+Irfan+i%2C+Hanaf&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.description=Crustasea+merupakan+sumber+kitin+yang+sangat+melimpah.+Melalui+proses+fermentasi%2C+kitin+diubah+menjadi+produk+turunannya%2C+yaitu+Chitooligosaccharide+(COS)%2C+yang+memanfaatkan+bahan+baku+dari+limbah+kulit+udang+dan+mikroorganisme+dekomposer+seperti+fungi.%0D%0APenelitian+ini+bertujuan+untuk+mengisolasi+dan+mengkarakterisasi+produk+biodegradasi+kitin+dari+kulit+udang+melalui+proses+fermentasi+padat+menggunakan+fungi.+Isolasi+COS+dilakukan+menggunakan+isolat+fungi+(18A12RF%2C+19A15RF%2C+20BA0502RF%2C+20CD01RF%2C+21RSM02+dan+22PLP1F1)+yang+diperoleh+dari+deposit+Unit+Pelaksanaan+Teknis+Laboratorium+Terpadu+Sentra+Inovasi+dan+Teknologi+(UPT+LTSIT)+sebagai+agen+pendegradasi+melalui+proses+kultivasi+secara+Solid+State+fermentation+(SSF)+selama+4+hari.+Produk+biodegradasi+oligomer+diekstraksi+menggunakan+air+panas%2C+dan+diisolasi+melalui+beberapa+tahap+kromatografi+serta+dianalisis+menggunakan+FTIR+dan+LC-MS%2FMS.+Produk+biodegradasi+ini+diuji+bioaktivitas+terhadap+bakteri+patogen+resisten+seperti+Staphylococcus+aureus%2C+dan+Pseudomonas+aeruginosa%2C+dan+fungi+Malassezia+globosa.%0D%0AHasil+penelitian+menunjukkan+bahwa+isolat+fungi+efektif+sebagai+agen+pendegradasi+kulit+udang.+Isolat+fungi+18A12RF+terbukti+menjadi+isolat+unggul+dalam+mendegradasi+kulit+udang+menjadi+COS+pada+hari+ke-4%2C+dibuktikan+dengan+uji+KLT.+Analisis+morfologi+menunjukan+isolat+18A12RF+merupakan+fungi+Aspergillus+sp.+COS+yang+dihasilkan+dari+degradasi+oleh+isolat+18A12RF+mampu+menghambat+pertumbuhan+mikroba+patogen+pada+dosis+2+mg%2FmL.+Karakterisasi+produk+hasil+degradasi+menggunakan+FTIR+dan+LC-MS%2FMS+mengindikasikan+bahwa+COS+memiliki+derajat+polimerisasi+(DP)+sebesar+7.%0D%0A%0D%0AKata+kunci%3A+antimikroba%2C+chitooligosaccharide%2C+enzim+kitinase%2C+isolat+fungi%2C+kulit+udang%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0A%0D%0Aabstract%0D%0A%0D%0ACrustaceans+are+a+highly+abundant+source+of+chitin.+Through+the+fermentation+process%2C+chitin+is+converted+into+its+derivative+product%2C+Chitooligosaccharide+(COS)%2C+utilizing+raw+materials+from+shrimp+shell+waste+and+decomposer+microorganisms+such+as+fungi.%0D%0AThis+study+aims+to+isolate+and+characterize+the+biodegradation+products+of+chitin+from+shrimp+shells+through+Solid+State+Fermentation+using+fungi.+COS+isolation+was+carried+out+using+fungal+isolates+(18A12RF%2C+19A15RF%2C+20BA0502RF%2C+20CD01RF%2C+22RSM02+and+22PLP1F1)+obtained+from+the+Integrated+Laboratory+Technical+Implementation+Unit+Center+for+Innovation+and+Technology+(UPT+LTSIT)+as+degrading+agents+through+Solid+State+Fermentation+(SSF)+cultivation+for+4+days.+The+oligomer+biodegradation+products+were+extracted+using+hot+water%2C+isolated+through+several+stages+of+chromatography%2C+and+analyzed+using+FTIR+and+LC-MS%2FMS.+The+biodegradation+products+were+tested+for+bioactivity+against+resistant+pathogenic+bacteria+such+as+Staphylococcus+aureus%2C+Pseudomonas+aeruginosa%2C+and+the+fungus+Malassezia+globosa.%0D%0AThe+results+showed+that+the+fungal+isolates+were+effective+as+shrimp+shell+degrading+agents.+The+fungal+isolate+18A12RF+proved+to+be+the+superior+isolate+in+degrading+shrimp+shells+into+COS+on+the+4+days%2C+as+evidenced+by+the+TLC+test.+Morphological+analysis+indicated+that+the+18A12RF+isolate+is+the+fungus+Aspergillus+sp.+The+COS+produced+from+the+degradation+by+the+18A12RF+isolate+was+able+to+inhibit+the+growth+of+pathogenic+microbes+at+a+dose+of+2+mg%2FmL.+Characterization+of+the+degradation+products+using+FTIR+and+LC-MS%2FMS+indicated+that+COS+has+a+degree+of+polymerization+(DP)+of+7.%0D%0A%0D%0AKeywords%3A+antimicrobial%2C+chitooligosaccharide%2C+chitinase+enzyme%2C+fungal+isolates%2C+shrimp+shells.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2024-08-07&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F81151%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F81151%2F2%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F81151%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++Muhammad+Irfan+i%2C+Hanaf++(2024)+BIOTRANSFORMASI+KULIT+UDANG+MENJADI+CHITOOLIGOSACCHARIDE+(COS)+OLEH+FUNGI+LAUT+DAN+UJI+BIOAKTIVITAS+SEBAGAI+ANTIMIKROBA.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F81151%2F