?url_ver=Z39.88-2004&rft_id=205701109&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=IMOBILISASI+ENZIM+LIPASE+DARI+BAKTERI+Pseudomonas+sp.+LPG171%0D%0ADENGAN+METODE+ADSORPSI+PADA+MATRIKS+HIDROKSIAPATIT&rft.creator=M.+Fahrezi+Cahaya+%2C+Saputra+&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.description=Kebutuhan+industri+yang+meningkat+terhadap+katalis+tidak+sebanding%0D%0Adengan+ketersediaannya%2C+sehingga+perlu+cara+yang+optimal+dalam+penggunaannya.%0D%0ASalah+satu+katalis+yang+efisien+dan+ekonomis+adalah+enzim+lipase.+Enzim+lipase%0D%0Amemiliki+aktivitas+katalitik+yang+tinggi+namun+tidak+bisa+digunakan+berulang+kali.%0D%0AUntuk+mengatasi+hal+tersebut%2C+solusi+yang+efektif+adalah+dengan+mengimobilisasi%0D%0Aenzim+tersebut%2C+agar+dapat+digunakan+secara+berulang.%0D%0APenelitian+ini+bertujuan+untuk+mengimobilisasi+enzim+lipase+dari+bakteri%0D%0APseudomonas+sp.+LPG171+melalui+metode+adsorpsi+pada+matriks+hidroksiapatit.%0D%0AProsedur+yang+dilakukan+meliputi%3A+peremajaan+bakteri%2C+produksi+enzim%2C+pemurnian%2C%0D%0Aimobilisasi+enzim%2C+dan+karakterisasi+enzim+lipase.+Aktivitas+hidrolisis+enzim+lipase%0D%0Adiukur+dengan+metode+Kwon+and+Rhee%2C+aktivitas+transesterifikasi+dengan+metode%0D%0AFu%2C+serta+kadar+protein+dengan+metode+Lowry.%0D%0AHasil+aktivitas+hidrolisis+pada+tahap+pemurnian+kromatografi+filtrasi+gel%0D%0Amenunjukkan+aktivitas+spesifik+80%2C15+U%2Fmg%2C+meningkat+kemurniannya+16%2C18+kali%0D%0Adibandingkan+dengan+ekstrak+kasar+yang+memiliki+aktivitas+spesifik+4%2C95+U%2Fmg.%0D%0AAktivitas+hidrolisis+enzim+lipase+yang+diimobilisasi+dan+enzim+lipase+bebas+tidak%0D%0Amengubah+kondisi+pH+dan+waktu+inkubasi%2C+tetapi+suhu+optimalnya+meningkat+dari%0D%0A35+%C2%B0C+menjadi+50+%C2%B0C.+Uji+pemakaian+berulang+menunjukkan+bahwa+aktivitas+enzim%0D%0Alipase+pada+reaksi+hidrolisis+menurun+dari+2%2C53+U%2FmL+menjadi+1%2C25+U%2FmL+setelah+5%0D%0Akali+pemakaian%2C+menunjukkan+penurunan+aktivitas+hingga+50%25+dari+aktivitas+awal.%0D%0AKata+kunci%3A+Pseudomonas+sp.+LPG171.%2C+lipase%2C+imobilisasi%2C+hidroksiapatit.%0D%0A%0D%0AThe+increasing+industrial+demand+for+catalysts+is+not+matched+by+their%0D%0Aavailability%2C+necessitating+optimal+methods+for+their+use.+One+efficient+and%0D%0Aeconomical+catalyst+is+lipase+enzyme.+Lipase+has+high+catalytic+activity+but+cannot%0D%0Abe+reused+multiple+times.+To+address+this+issue%2C+an+effective+solution+is+to%0D%0Aimmobilize+the+enzyme%2C+allowing+it+to+be+used+repeatedly.%0D%0AThis+research+aims+to+immobilize+lipase+enzyme+from+Pseudomonas+sp.%0D%0ALPG171+bacteria+using+an+adsorption+method+on+hydroxyapatite+matrix.+The%0D%0Aprocedures+involved+include%3A+bacterial+rejuvenation%2C+enzyme+production%2C%0D%0Apurification%2C+enzyme+immobilization%2C+and+characterization+of+lipase+enzyme.+The%0D%0Ahydrolysis+activity+of+lipase+enzyme+is+measured+using+the+Kwon+and+Rhee+method%2C%0D%0Atransesterification+activity+is+measured+using+the+Fu+method%2C+and+protein%0D%0Aconcentration+is+determined+using+the+Lowry+method.%0D%0ATransesterification+activity+in+gel+filtration+chromatography+showed+unit%0D%0Aactivity+of+31%2C08+U%2FmL.+While+hydrolysis+activity+at+the+purification+stage+using+gel%0D%0Afiltration+chromatography+showed+a+specific+activity+of+80.15+U%2Fmg%2C+increasing+its%0D%0Apurity+by+16.18+times+compared+to+the+crude+extract%2C+which+had+a+specific+activity%0D%0Aof+4.95+U%2Fmg.+The+hydrolysis+activity+of+both+immobilized+lipase+enzyme+and+free%0D%0Alipase+enzyme+did+not+change+the+pH+conditions+and+incubation+time%2C+but+the%0D%0Aoptimal+temperature+increased+from+35+%C2%B0C+to+50+%C2%B0C.+The+repeated+use+test+showed%0D%0Athat+the+activity+of+lipase+enzyme+in+the+hydrolysis+reaction+decreased+from+2.53%0D%0AU%2FmL+to+1.25+U%2FmL+after+5+uses%2C+indicating+a+50%25+reduction+in+activity+from+the%0D%0Ainitial+value.%0D%0AKeywords%3A+Pseudomonas+sp.+LPG171.%2C+lipase%2C+immobilization%2C+hydroxyapatite.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2024-10-18&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F81195%2F1%2FABSTRAK.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F81195%2F3%2FSKRIPSI%2520FULL.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F81195%2F2%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN.pdf&rft.identifier=++M.+Fahrezi+Cahaya+%2C+Saputra+++(2024)+IMOBILISASI+ENZIM+LIPASE+DARI+BAKTERI+Pseudomonas+sp.+LPG171+DENGAN+METODE+ADSORPSI+PADA+MATRIKS+HIDROKSIAPATIT.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.++++(Submitted)++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F81195%2F