?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=PROFIL+SENYAWA+BIOAKTIF+EKSTRAK+DAUN+PECUT+KUDA%0D%0A(Stachytarpheta+australis)+SEBAGAI+ANTIBAKTERI+TERHADAP%0D%0ABAKTERI+PATOGEN+Staphylococcus+aureus+DAN+Pseudomonas+aeruginosa%0D%0A%0D%0AMENGGUNAKAN+LC-MS%2FMS&rft.creator=Fayza+%2C+Azzahra&rft.subject=502+Aneka+ragam+tentang+ilmu+pengetahuan+alam&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.description=Kasus+resistensi+bakteri+terhadap+antibiotik+semakin+meningkat+di+seluruh+dunia%0D%0Asejak+beberapa+dekade+terakhir.+Hal+ini+mendorong+para+ilmuan+untuk+mengeksplor%0D%0Aalternatif+pengobatan+lain+yang+lebih+aman+dan+efektif%2C+yaitu+dengan+menggunakan%0D%0Atanaman+obat+herbal.+Salah+satu+jenis+tanaman+obat+yang+telah+banyak+diteliti+dan%0D%0Adilaporkan+memiliki+efek+antibakteri+adalah+tanaman+Pecut+Kuda+(Stachytarpheta%0D%0Ajamaicensis).+Spesies+pecut+kuda+sangat+beragam+dan+diantaranya+adalah+Pecut%0D%0AKuda+(Stachytarpheta+australis)+yang+memiliki+bunga+berwarna+putih%2C+hanya+saja%0D%0Ainformasi+mengenai+aktivitas+antibakterinya+masih+sangat+terbatas.+Penelitian+ini%0D%0Adilakukan+dengan+tujuan+untuk+menguji+potensi+ekstrak+daun+Pecut+Kuda%0D%0A(Stachytarpheta+australis)+sebagai+antibakteri+terhadap+Staphylococcus+aureus+dan%0D%0APseudomonas+aeruginosa+dengan+metode+KLT+Bioautografi+dan+metode+Difusi%0D%0AAgar%2C+serta+menganalisis+profil+senyawa+bioaktif+yang+terdapat+di+dalam+ekstrak%0D%0Atanaman+menggunakan+metode+Liquid+chromatography-tandem+mass+spectrometry%0D%0A(LC-MS%2FMS).+Hasil+uji+KLT+Bioautografi+mengindikasikan+ekstrak+kasar%2C+fraksi%0D%0Abutanol+kode+FPKAB2%2C+dan+FPKAB3+mampu+menghambat+pertumbuan+bakteri+S.%0D%0Aaureus.+Hasil+uji+antibakteri+metode+difusi+agar+menunjukkan+bahwa+fraksi%0D%0AFPKAB3+memiliki+aktivitas+antibakteri+yang+tergolong+kuat+yaitu+dengan+diameter%0D%0Azona+hambat+yaitu+12+mm+pada+bakteri+S.+aureus%2C+dan+16+mm+pada+bakteri+P.%0D%0Aaeruginosa.+Hasil+karakterisasi+dengan+LC-MS%2FMS+diperoleh+profil+senyawa%0D%0Abioaktif+dari+ekstrak+air+daun+pecut+kuda+(Stachytarpheta+australis)+yang+berperan%0D%0Adalam+aktivitas+antibakteri+terhadap+Staphylococcus+aureus+dan+Pseudomonas%0D%0Aaeruginosa+yaitu+senyawa+Diethyl+bis+(%7B%5B(4-methyl-1%2C2%2C5-oxadiazol-3-yl)%0D%0Acarbamoyl%5D+oxy%7Dmethyl)+malonate+dengan+berat+molekul+471%2C1476+m%2Fz+terdeteksi%0D%0Apada+waktu+retensi+5%2C44+menit.%0D%0A%0D%0AKata+kunci+%3A+Bakteri+patogen%2C+Senyawa+bioaktif%2C+Antibakteri%2C+LC-MS%2FMS%0D%0ACases+of+bacterial+resistance+to+antibiotics+have+been+increasing+around+the+world%0D%0Asince+the+last+few+decades.+This+encourages+scientists+to+explore+other+safer+and%0D%0Amore+effective+treatment+alternatives%2C+that+was+by+using+herbal+medicinal+plants.%0D%0AOne+type+of+medicinal+plant+that+has+been+widely+researched+and+reported+to+have%0D%0Aantibacterial+effects+is+the+pecut+kuda+plant+(Stachytarpheta+jamaicensis).+Pecut%0D%0Akuda+are+very+diverse+and+among+them+is+the+pecut+kuda+(Stachytarpheta+australis)%0D%0Awhich+has+white+flowers%2C+but+information+about+its+antibacterial+activity+is+still+very%0D%0Alimited.+This+study+was+conducted+with+the+aim+of+testing+the+potential+of+pecut%0D%0Akuda+leaf+extract+(Stachytarpheta+australis)+as+an+antibacterial+against%0D%0AStaphylococcus+aureus+and+Pseudomonas+aeruginosa+by+the+Bioautography+TLC%0D%0Amethod+and+the+Agar+Diffusion+method%2C+as+well+as+analyzing+the+profile+of+bioactive%0D%0Acompounds+contained+in+plant+extracts+using+the+Liquid+chromatography-tandem%0D%0Amass+spectrometry+(LC-MS%2FMS)+method.+The+results+of+the+Bioautography+TLC%0D%0Atest+indicated+that+crude+extracts%2C+butanol+fractions+coded+FPKAB2%2C+and+FPKAB3%0D%0Awere+able+to+inhibit+the+growth+of+S.+aureus+bacteria.+The+results+of+the+Agar%0D%0ADiffusion+method+showed+that+the+FPKAB3+fraction+had+a+relatively+strong%0D%0Aantibacterial+activity%2C+with+an+inhibitory+zone+diameter+of+12+mm+in+S.+aureus%0D%0Abacteria%2C+and+16+mm+in+P.+aeruginosa+bacteria.+The+results+of+characterization+with%0D%0ALC-MS%2FMS+obtained+a+bioactive+compound+profile+of+pecut+kuda+leaf+water+extract%0D%0A(Stachytarpheta+australis)+which+plays+a+role+in+antibacterial+activity+against%0D%0AStaphylococcus+aureus+and+Pseudomonas+aeruginosa%2C+namely+the+compound%0D%0ADiethyl+bis(%7B%5B(4-methyl-1%2C2%2C5-oxadiazol-3-yl)carbamoyl%5Doxy%7Dmethyl)+malonate%0D%0Awith+a+molecular+weight+of+471.1476+m%2Fz+was+detected+at+a+retention+time+of+5.44%0D%0Aminutes.%0D%0A%0D%0AKeyword+%3A+Pathogenic+bacteria%2C+Bioactive+compounds%2C+Antibacterial%2C+LC-MS%2FMS&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2024-08-13&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F81845%2F1%2Fabstrak%2520Fayza%2520-%2520Fayza%2520Azzahra.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F81845%2F2%2FTANPA%2520LAMPIRAN%2520-%2520Fayza%2520Azzahra.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigil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