?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=IMOBILISASI+ENZIM+%CE%B1-AMILASE+DARI+Aspergillus+sp.%0D%0A%0D%0AMENGGUNAKAN+BENTONIT&rft.creator=Stephani+%2C+Marisca+Febrianti%09&rft.subject=001+Ilmu+pengetahuan&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.description=Kestabilan+enzim+menjadi+faktor+penting+dalam+proses+industri+karena%0D%0Adibutuhkan+enzim+yang+dapat+bekerja+pada+pH+dan+suhu+ekstrem+serta+memiliki%0D%0Amasa+pakai+yang+lama+agar+lebih+ekonomis+dan+dapat+mengurangi+biaya+produksi.%0D%0ANamun%2C+umumnya%2C+enzim+tidak+dapat+bekerja+secara+optimal+pada+pH+dan+suhu%0D%0Atersebut.+Oleh+karena+itu%2C+perlu+dilakukan+peningkatan+kestabilan+enzim+untuk%0D%0Amengatasi+masalah+ini.%0D%0APenelitian+ini+bertujuan+untuk+meningkatkan+kestabilan+enzim+%CE%B1-amilase%0D%0Adari+Aspergillus+sp.+dengan+imobilisasi+menggunakan+matriks+bentonit.+Tahapan%0D%0Apenelitian+meliputi+produksi+dan+isolasi+enzim%2C+pemurnian+enzim+melalui%0D%0Afraksinasi+menggunakan+amonium+sulfat+dan+dialisis%2C+serta+imobilisasi+enzim+hasil%0D%0Apemurnian+menggunakan+matriks+bentonit.+Selanjutnya%2C+baik+enzim+hasil%0D%0Apemurnian+maupun+enzim+hasil+imobilisasi+dikarakterisasi.%0D%0AHasil+penelitian+menunjukkan+bahwa+enzim+hasil+pemurnian+memiliki%0D%0Apeningkatan+kemurnian+11+kali+dibandingkan+dengan+ekstrak+kasar+enzim.+Uji%0D%0Astabilitas+termal+pada+suhu+60%C2%B0C+selama+80+menit+untuk+enzim+hasil+pemurnian%0D%0Amenunjukkan+aktivitas+sisa+sebesar+23%25+dengan+waktu+paruh+sebesar+39%2C38+menit%2C%0D%0Asedangkan+enzim+hasil+imobilisasi+dengan+matriks+bentonit+menunjukkan+aktivitas%0D%0Asisa+sebesar+47%25+dengan+waktu+paruh+sebesar+76%2C17+menit%2C+pada+kondisi+yang%0D%0Asama.+Kestabilan+enzim+hasil+imobilisasi+memiliki+peningkatan+sebesar+1%2C93+kali%0D%0Adibandingkan+dengan+enzim+hasil+pemurnian.+Aktivitas+sisa+enzim+hasil%0D%0Aimobilisasi+setelah+pemakaian+berulang+sebanyak+enam+kali+menunjukkan+nilai%0D%0Asebesar+48%25.%0D%0AKata+kunci%3A+%CE%B1-amilase%2C+Aspergillus+sp.%2C+imobilisasi%2C+bentonit%0D%0AEnzyme+stability+is+a+crucial+factor+in+industrial+processes+because+enzymes+need%0D%0Ato+function+at+extreme+pH+and+temperature+levels+and+have+a+long+lifespan+to+be%0D%0Amore+economical+and+reduce+production+costs.+However%2C+enzymes+generally%0D%0Acannot+function+optimally+at+these+pH+and+temperature+conditions.+Therefore%2C+it+is%0D%0Anecessary+to+enhance+the+stability+of+enzymes+to+address+this+issue.%0D%0AThis+research+aims+to+enhance+the+stability+of+%CE%B1-amylase+enzyme+from%0D%0AAspergillus+sp.+through+the+immobilization+using+a+bentonite+matrix.+The+research%0D%0Astages+include+enzyme+production+and+isolation%2C+enzyme+purification+through%0D%0Aammonium+sulfate+fractionation+and+dialysis%2C+and+immobilization+of+the+purified%0D%0Aenzyme+using+a+bentonite+matrix.+Subsequently%2C+both+the+purified+enzyme+and+the%0D%0Aimmobilized+enzyme+are+characterized.%0D%0AThe+research+results+show+that+the+purified+enzyme+had+an+11-fold+increase+in%0D%0Apurity+compared+to+the+crude+extract.+Thermal+stability+tests+at+60%C2%B0C+for%0D%0A80+minutes+showed+that+the+purified+enzyme+retained+23%25+residual+activity+with+a%0D%0Ahalf-life+of+39.38+minutes%2C+while+the+immobilized+enzyme+with+bentonite+retained%0D%0A47%25+residual+activity+with+a+half-life+of+76.17+minutes.+The+stability+of+the%0D%0Aimmobilized+enzyme+increased+by+1.93+times+compared+to+the+purified+enzyme.%0D%0AThe+immobilized+enzyme+retained+48%25+residual+activity+after+six+repeated+uses.%0D%0AKeywords%3A+%CE%B1-amylase%2C+Aspergillus+sp.%2C+immobilization%2C+bentonite&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+&rft.date=2024-08-08&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F81904%2F1%2FStephani%2520Marisca%2520Febrianti_Abstrak%2520-%2520Stephani%2520Marisca.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F81904%2F2%2FStephani%2520Marisca%2520Febrianti_full%2520-%2520Stephani%2520Marisca.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F81904%2F3%2FStephani%2520Marisca%2520Febrianti_tanpa%2520bab%2520pembahasan%2520-%2520Stephani%2520Marisca.pdf&rft.identifier=++Stephani+%2C+Marisca+Febrianti+++(2024)+IMOBILISASI+ENZIM+%CE%B1-AMILASE+DARI+Aspergillus+sp.+MENGGUNAKAN+BENTONIT.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM+%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F81904%2F