?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=PENGARUH+PENAMBAHAN+GLISEROL+TERHADAP+AKTIVITAS%0D%0AENZIM+PROTEASE+DARI+BAKTERI+Klebsiella+sp.+LPG172&rft.creator=PUTRI+%2C+AYU+SAFITRI&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=540+Kimia+dan+ilmu+yang+berhubungan&rft.description=Penelitian+ini+bertujuan+untuk+mengetahui+pengaruh+gliserol+terhadap+aktivitas%0D%0Aenzim+protease+dari+bakteri+Klebsiella+sp.+LPG172.+Produksi+enzim+protease%0D%0Adilakukan+dengan+menumbuhkan+Klebsiella+sp.+LPG172+pada+media+dengan+1%25%0D%0Asusu+skim+yang+dilarutkan+dalam+larutan+buffer+fosfat+0%2C05+M+pada+pH+7.+Enzim%0D%0Adipanen+setelah+fermentasi+selama+42+jam+dan+dimurnikan+melalui+fraksinasi%0D%0Aammonium+sulfat+serta+dialisis.+Aktivitas+enzim+dihitung+dengan+metode+Kunitz%0D%0Adan+kadar+protein+ditentukan+dengan+metode+Lowry.+Karakterisasi+enzim+mnencakup%0D%0Apengujian+pH%2C+suhu%2C+dan+waktu+inkubasi+optimum.+Aktivitas+optimum+protease%0D%0Atercapai+pada+pH+7%2C+suhu+50%C2%B0C%2C+dan+waktu+inkubasi+40+menit.+Enzim+hasil+dialisis%0D%0Adiuji+tanpa+dan+dengan+penambahan+gliserol+pada+konsentrasi+0%2C5+M%2C+1%2C0+M%2C+dan+1%2C5%0D%0AM.+Pada+pH+7%2C+penambahan+gliserol+0%2C5+M%2C+1%2C0+M%2C+dan+1%2C5+M+meningkatkan%0D%0Aaktivitas+enzim+masing-masing+sebesar+5%2C00%25%2C+18%2C18%25%2C+dan+8%2C36%25.+Pada+suhu%0D%0A50%C2%B0C%2C+penambahan+gliserol+0%2C5+M%2C+1%2C0+M%2C+dan+1%2C5+M+meningkatkan+aktivitas+enzim%0D%0Asebesar+2%2C46%25%2C+14%2C26%25%2C+dan+8%2C11%25.+Pada+waktu+inkubasi+40+menit%2C+peningkatan%0D%0Aaktivitas+enzim+dengan+penambahan+gliserol+0%2C5+M%2C+1%2C0+M%2C+dan+1%2C5+M+adalah+5%2C40%25%2C%0D%0A8%2C88%25%2C+dan+18%2C79%25.+Hasil+menunjukkan+bahwa+penambahan+gliserol+mampu%0D%0Ameningkatkan+aktivitas+enzim+protease+dari+Klebsiella+sp.+LPG172+tanpa+mengubah%0D%0Akondisi+kerja+optimum+enzim%2C+yaitu+pada+pH+7%2C+suhu+50%C2%B0C%2C+dan+waktu+inkubasi+40%0D%0Amenit.+Konsentrasi+gliserol+1%2C0+M+memberikan+peningkatan+aktivitas+yang%0D%0Akonsisten+dan+signifikan.%0D%0AKata+kunci%3A+protease%2C+Klebsiella+sp%2C+gliserol.%0D%0A%0D%0A%0D%0AThis+study+aims+to+investigate+the+effect+of+glycerol+on+the+activity+of+the+protease%0D%0Aenzyme+from+Klebsiella+sp.+LPG172.+Protease+enzyme+production+was+conducted%0D%0Aby+cultivating+Klebsiella+sp.+LPG172+ina+medium+containing+1%25+skim+milk%0D%0Adissolved+in+0.05+M+phosphate+buffer+solution+at+pH+7.+The+enzyme+was+harvested%0D%0Aafter+42+hours+of+fermentation+and+purified+through+ammonium+sulfate%0D%0Afractionation+and+dialysis.+Enzyme+activity+was+measured+using+the+Kunitz%0D%0Amethod%2C+and+protein+content+was+determined+using+the+Lowry+method.+Enzyme%0D%0Acharacterization+included+determining+the+optimal+conditions+for+enzyme+activity%2C%0D%0Aincluding+pH%2C+temperature%2C+and+incubation+time.+The+optimal+activity+of+the%0D%0Aprotease+was+achieved+at+pH7%2C+50%C2%B0C%2C+and+40+minutes+of+incubation+time.+The%0D%0Adialyzed+enzyme+was+tested+without+and+with+the+addition+of+glycerol+at%0D%0Aconcentrations+of+0.5+M%2C+1.0+M%2C+and+1.5+M.+At+pH+7%2C+the+addition+of+0.5+M%2C+1.0+M%2C%0D%0Aand+1.5+M+glycerol+increased+enzyme+activity+by+5.00%25%2C+18.18%25%2C+and+8.36%25%2C%0D%0Arespectively.+At+50%C2%B0C%2C+the+addition+of+0.5+M%2C+1.0+M%2C+and+1.5+M+glycerol+increased%0D%0Aenzyme+activity+by+2.46%25%2C+14.26%25%2C+and+8.11%25%2C+respectively.+At+40+minutes+of%0D%0Aincubation%2C+the+addition+of+0.5+M%2C+1.0+M%2C+and+1.5+M+glycerol+increased+enzyme%0D%0Aactivity+by+5.40%25%2C+8%2C88%25%2C+and+18.79%25%2C+respectively.+The+results+show+that+the%0D%0Aaddition+of+glycerol+can+enhance+the+activity+of+the+protease+enzyme+from%0D%0AKlebsiella+sp.+LPG172+without+altering+the+enzyme's+optimal+working+conditions%2C%0D%0Awhich+are+pH+7%2C+50%C2%B0C%2C+and+40+minutes+of+incubation+time.+The+1.0+M+glycerol%0D%0Aconcentration+consistently+and+significantly+increased+enzyme+activity.%0D%0AKeyword%3A+protease%2C+Klebsiella+sp%2C+glycerol.%0D%0A&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2024-07-24&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F82072%2F1%2F1917011092%2520-%2520Putri%2520Ayu%2520Safitri%2520-%2520Abstrak%2520-%2520Putri%2520Ayuu.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F82072%2F2%2FPutri%2520ayu%2520safitri%2520-%25201917011092%2520-%2520skripsi%2520tanpa%2520lampiran%2520-%2520Putri%2520Ayuu.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F82072%2F3%2FPutri%2520ayu%2520safitri%2520-%25201917011092%2520-%2520skripsi%2520tanpa%2520pembahasan%2520dan%2520lampiran%2520-%2520Putri%2520Ayuu.pdf&rft.identifier=++PUTRI+%2C+AYU+SAFITRI++(2024)+PENGARUH+PENAMBAHAN+GLISEROL+TERHADAP+AKTIVITAS+ENZIM+PROTEASE+DARI+BAKTERI+Klebsiella+sp.+LPG172.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F82072%2F