?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=IMOBILISASI+ENZIM+LIPASE+DARI+BAKTERI+Pseudomonas+sp.+LPG171%0D%0A%0D%0ADENGAN+MATRIKS+ZEOLIT+ALAM+TERAKTIVASI&rft.creator=Bunga+%2C+Mega+Nurlinda&rft.subject=600+Teknologi+(ilmu+terapan)&rft.subject=660+Teknik+kimia&rft.description=Imobilisasi+enzim+dilakukan+untuk+meningkatkan+stabilitas+enzim+dengan%0D%0Amengurangi+kelarutan+enzim+dalam+air+karena+pergerakan+molekul+enzim+yang%0D%0Aditahan+dengan+matriks+yang+tidak+larut+dalam+air+seperti+zeolit.+Dalam+penelitian%0D%0Aini+dilakukan+imobilisasi+enzim+lipase+dari+bakteri+Pseudomonas+sp.+LPG171.%0D%0ATahapan+penelitian+yang+dilakukan+meliputi+produksi+enzim%2C+pemurnian+ekstrak%0D%0Akasar+enzim%2C+imobilisasi%2C+karakterisasi+enzim+terimobilisasi+dan+penggunaan%0D%0Aberulang+enzim+terimobilisasi.+Aktivitas+hidrolisis+ditentukan+dengan+metode+Kwon%0D%0Aand+Rhee+dengan+substrat+minyak+zaitun+sementara+aktivitas+transester+ditentukan%0D%0Adengan+metode+Fuu+dengan+substrat+P-Nitrofenol+Palmitat.%0D%0AHasil+penelitian+menunjukkan+bahwa+enzim+hasil+pemurnian+memiliki%0D%0Aaktivitas+spesifik+22%2C29+kali+lebih+tingi+yaitu+480%2C44+U%2Fmg+dibandingkan+ekstrak%0D%0Akasar+enzim+yang+memiliki+aktivitas+spesifik+sebesar+21%2C25+U%2Fmg.Imobilisasi+enzim%0D%0Alipase+secara+keseluruhan+tidak+mengubah+kondisi+optimum+kecuali+aktivitas%0D%0Aoptimum+lipase+terimobil+pada+suhu+40%C2%B0C%2C+5%C2%B0C+lebih+tinggi+dibandingkan+suhu%0D%0Aoptimum+enzim+bebas.+Enzim+lipase+yang+telah+terimobilisasi+dengan+zeolit+alam%0D%0Ateraktivasi+memiliki+kondisi+optimum+pada+aktivitas+transester+yang+berada+pada%0D%0ApH+7%2C+suhu+40%C2%B0C%2C+dan+waktu+inkubasi+10+menit.+Berdasarkan+hasil+yang+diperoleh%0D%0Aenzim+lipase+terimobil+dengan+zeolit+alam+teraktivasi+memiliki+aktivitas+awal%0D%0Asebesar+132%2C40+U%2F+mL+namun+setelah+pemakaian+yang+ke-5+kali+reaksi%0D%0Atransesterifikasi+aktivitas+enzim+lipase+terimobil+mengalami+penurunan+sebesar+60%0D%0AU%2FmL+yang+mana+hal+ini+menunjukkan+enzim+lipase+terimobil+telah+kehilangan%0D%0Aaktivitasnya+sebesar+43%2C31%25.%0D%0A%0D%0AKata+kunci%3A+Pseudomonas+sp.%2C+lipase%2C+imobilisasi%2C+dan+zeolit+alam.%0D%0A%0D%0AEnzyme+immobilization+is+carried+out+to+improve+enzyme+stability+by+reducing+the%0D%0Asolubility+of+enzymes+in+water+due+to+the+movement+of+enzyme+molecules+that+are%0D%0Aheld+in+water-insoluble+matrices+such+as+zeolites.+In+this+study%2C+the+enzyme+lipase%0D%0Awas+immobilized+from+the+bacterium+Pseudomonas+sp.+LPG171.+The+stages+of%0D%0Aresearch+conducted+included+enzyme+production%2C+purification+of+raw+enzyme%0D%0Aextracts%2C+immobilization%2C+characterization+of+immobilized+enzymes%2C+and+repeated%0D%0Asuspension+of+the+immobilized+enzymes.+The+hydrolysis+activity+is+determined+by%0D%0Athe+Kwon+and+Rhee+method+with+an+olive+oil+substrate%2C+while+the+transester+activity%0D%0Ais+measured+by+the+Fuu+method+with+the+P-Nitrophenol+Palmitat+substrat.+The%0D%0Aresults+showed+that+purification+enzymes+have+a+22.29+times+higher+specific+activity%0D%0Aof+480.44+U%2Fmg+compared+to+raw+enzyme+extracts+that+have+a+21.25+U%2F+mg+specific%0D%0Aactivity.+The+overall+immobilization+of+the+lipase+enzyme+does+not+alter+the+optimal%0D%0Acondition+unless+the+optimum+activity+of+the+movable+lipase+is+at+a+temperature+of%0D%0A40%C2%B0C%2C+5%C2%B0C+higher+than+the+optimal+temperature+of+the+free+enzyme.+The+enzyme%0D%0Alipase+that+has+been+immobilized+with+the+activated+natural+zeolite+has+optimum%0D%0Aconditions+for+transester+activity+at+pH+7%2C+temperature+40%C2%B0C%2C+and+incubation+time+of%0D%0A10+minutes.+Based+on+the+results+obtained%2C+the+enzyme+lipase+mobilized+with%0D%0Aactivated+natural+zeolite+has+an+initial+activity+of+132.40+U%2F+mL+but+after+the+fifth%0D%0Ause+the+transesterification+reaction+activity+of+the+enzyme+lipase+movable+has%0D%0Adecreased+by+60+U%2FmL+which+indicates+that+the+activated+lipase+enzyme+has+lost+its%0D%0Aactivity+by+43.31%25.%0D%0A%0D%0AKey+words%3A+Pseudomonas+sp.%2C+lipase%2C+immobilization%2C+and+natural+zeolite.&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2024-07-30&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F82217%2F1%2F2017011071_Bunga%2520Mega%2520Nurlinda_Abstrak%2520-%2520Bunga%2520Mega.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F82217%2F2%2F2017011071_Bunga%2520Mega%2520Nurlinda_Skripsi%2520Full%2520-%2520Bunga%2520Mega.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F82217%2F3%2F2017011071_Bunga%2520Mega%2520Nurlinda_Skripsi%2520Tanpa%2520Bab%2520Pembahasan%2520-%2520Bunga%2520Mega.pdf&rft.identifier=++Bunga+%2C+Mega+Nurlinda++(2024)+IMOBILISASI+ENZIM+LIPASE+DARI+BAKTERI+Pseudomonas+sp.+LPG171+DENGAN+MATRIKS+ZEOLIT+ALAM+TERAKTIVASI.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F82217%2F