?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=IMOBILISASI+ENZIM+%CE%B1-AMILASE+DARI+Aspergillus+sp.%0D%0A%0D%0AMENGGUNAKAN+ZEOLIT&rft.creator=Maria+%2C+Agustina+Sidabutar&rft.subject=500+ilmu+pengetahuan+alam+dan+matematika&rft.subject=510+Matematika&rft.description=menghidrolisis+ikatan+%CE%B1-1%2C4-glikosidik.+Enzim+ini+banyak+dimanfaatkan+dalam%0D%0Aindustri+kimia.+Penggunaan+enzim+dalam+industri+harus+memenuhi+beberapa+kriteria%0D%0Akhusus%2C+di+antaranya+memiliki+kestabilan+pada+kondisi+suhu+yang+tinggi+dan+pH%0D%0Ayang+ekstrem%2C+namun+umumnya+enzim+tidak+dapat+bekerja+secara+optimum+pada%0D%0Akondisi+suhu+dan+pH+tersebut.+Sehingga+perlu+dilakukan+peningkatan+kestabilan%0D%0Aenzim+untuk+mengatasi+masalah+ini.%0D%0APenelitian+ini+bertujuan+untuk+meningkatkan+stabilitas+enzim+%CE%B1-amilase+dari%0D%0AAspergillus+sp.+dengan+imobilisasi+menggunakan+matriks+zeolit.+Untuk+mencapai%0D%0Atujuan+tersebut+maka+enzim+%CE%B1-amilase+diisolasi%2C+diproduksi%2C+dan+dimurnikan+dengan%0D%0Ametode+fraksinasi+menggunakan+amonium+sulfat%2C+dilanjutkan+dengan+dialisis.%0D%0AEnzim+hasil+pemurnian+diimobilisasi+menggunakan+zeolit+sebagai+matriks%0D%0Apendukung.+Kemudian%2C+enzim+hasil+pemurnian+dan+imobilisasi+dikarakterisasi.%0D%0AHasil+penelitian+menunjukkan+bahwa+enzim+%CE%B1-amilase+hasil+pemurnian%0D%0Amemiliki+tingkat+kemurnian+sebesar+13+kali+lipat+dibandingkan+dengan+ekstrak%0D%0Akasar+enzim.+Uji+stabilitas+termal+pada+enzim+%CE%B1-amilase+hasil+pemurnian+selama+80%0D%0Amenit+pada+suhu+60%C2%B0C+menunjukkan+aktivitas+sisa+sebesar+23%25+dan+waktu+paruh%0D%0Asebesar+43%2C32+menit%2C+sedangkan+enzim+hasil+imobilisasi+menggunakan+matriks%0D%0Azeolit+menunjukkan+aktivitas+sisa+sebesar+49%25+dan+waktu+paruh+sebesar+78%2C76%0D%0Amenit.+Enzim+hasil+imobilisasi+mengalami+peningkatkan+kestabilan+sebesar+1%2C82%0D%0Akali+dibandingkan+enzim+hasil+pemurnian.+Pada+pemakaian+berulang+enzim+hasil%0D%0Aimobilisasi+masih+menunjukkan+aktivitas+sisa+sebesar+35%25+setelah+penggunaan%0D%0Asebanyak+enam+kali.%0D%0AKata+kunci+%3A+%CE%B1-amilase%2C+Aspergillus+sp.%2C+imobilisasi%2C+zeolit%0D%0A%0D%0A%0D%0A%0D%0AThe+enzyme+%CE%B1-amylase+belongs+to+the+hydrolase+group%2C+which+can%0D%0Ahydrolyze+%CE%B1-1%2C4-glycosidic+bonds.+This+enzyme+is+widely+utilized+in+the+chemical%0D%0Aindustry.+The+use+of+enzymes+in+industry+must+meet+several+specific+criteria%2C%0D%0Aincluding+stability+under+high-temperature+conditions+and+extreme+pH+levels.%0D%0AHowever%2C+enzymes+generally+cannot+function+optimally+under+these+extreme%0D%0Aconditions.+Therefore%2C+it+is+necessary+to+enhance+enzyme+stability+to+overcome+this%0D%0Aissue.+Then%2C+the+purified+and+immobilized+enzymes+were+characterized.%0D%0AThis+research+aims+to+increase+the+stability+of+%CE%B1-amylase+from+Aspergillus%0D%0Asp.+by+immobilization+using+a+zeolite+matrix.+To+achieve+this+goal%2C+the+%CE%B1-amylase%0D%0Aenzyme+was+isolated%2C+produced%2C+and+purified+using+fractionation+with+ammonium%0D%0Asulfate%2C+and+followed+by+dialysis.+The+purified+enzyme+was+immobilized+using%0D%0Azeolite+as+a+supporting+matrix.+Then%2C+the+purified+and+immobilized+enzymes+were%0D%0Acharacterized.%0D%0AThe+research+showed+that+the+purified+%CE%B1-amylase+enzyme+had+a+purity+level%0D%0A13+times+higher+than+the+crude+enzyme+extract.+Thermal+stability+tests+on+the%0D%0Apurified+%CE%B1-amylase+enzyme+for+80+minutes+at+60%C2%B0C+showed+a+residual+activity+of%0D%0A23%25+and+a+half-life+of+43.32+minutes%2C+meanwhile%2C+the+immobilized+enzyme+using+a%0D%0Azeolite+matrix+exhibited+a+residual+activity+of+49%25+and+a+half-life+of+78.76+minutes.%0D%0AThe+immobilized+enzyme+showed+a+1.82-fold+increase+in+stability+compared+to+the%0D%0Apurified+enzyme.+After+six+repeated+uses%2C+the+immobilized+enzyme+still+retained%0D%0A35%25+of+its+residual+activity.%0D%0AKeywords%3A+%CE%B1-amylase%2C+Aspergillus+sp.%2C+immobilization%2C+zeolite&rft.publisher=FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM&rft.date=2024-08-07&rft.type=Skripsi&rft.type=NonPeerReviewed&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F82421%2F1%2FABSTRAK-ABSTRACT%2520-%2520Maria%2520Agustina.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F82421%2F2%2FSKRIPSI%2520FULL%2520-%2520Maria%2520Agustina.pdf&rft.format=text&rft.identifier=http%3A%2F%2Fdigilib.unila.ac.id%2F82421%2F3%2FSKRIPSI%2520TANPA%2520BAB%2520PEMBAHASAN%2520-%2520Maria%2520Agustina.pdf&rft.identifier=++Maria+%2C+Agustina+Sidabutar++(2024)+IMOBILISASI+ENZIM+%CE%B1-AMILASE+DARI+Aspergillus+sp.+MENGGUNAKAN+ZEOLIT.++FAKULTAS+MATEMATIKA+DAN+ILMU+PENGETAHUAN+ALAM%2C+UNIVERSITAS+LAMPUNG.+++++&rft.relation=http%3A%2F%2Fdigilib.unila.ac.id%2F82421%2F